Sharpea azabuensis : a ruminal bacterium that produces trans-11 intermediates from linoleic and linolenic acid
- Author
- Lore Dewanckele (UGent) , Bruno Vlaeminck (UGent) and Veerle Fievez (UGent)
- Organization
- Abstract
- To investigate the metabolism of 18: 2n-6 and 18: 3n-3 by pure cultures of Sharpea azabuensis, two different strains ( RL 1 and ST18) were each incubated in the presence of 40 mu g ml(-1) 18: 2n-6 or 18: 3n-3. Pure cultures of Butyrivibrio fibrisolvens D1 and Butyrivibrio proteoclasticus P18 were included as control treatments. Similar to the metabolism of B. fibrisolvens, both S. azabuensis strains converted 18: 2n-6 or 18: 3n-3 to cis-9, trans-11 CLA or cis-9, trans-11, cis-15 CLnA, after which it was further reduced to trans-11 18: 1 or trans-11, cis-15 18: 2, respectively. B. proteoclasticus additionally reduced trans-11 18: 1 to 18: 0. Trans-11, cis-15 18: 2 was also further metabolized by B. proteoclasticus, although trans-11 18: 1 did not accumulate, and only minor amounts of 18: 0 were formed. The time frame of 18: 2n-6 and 18: 3n-3 biohydrogenation by S. azabuensis was comparable with B. fibrisolvens, indicating that S. azabuensis and B. fibrisolvens might be alternative biohydrogenators of 18: 2n-6 and 18: 3n-3 in the rumen.
- Keywords
- biohydrogenation, linoleic acid, linolenic acid, Sharpea azabuensis, FATTY-ACIDS, COMMUNITY COMPOSITION, BIOHYDROGENATION, METABOLISM, ISOMERS, RUMEN
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8627614
- MLA
- Dewanckele, Lore, et al. “Sharpea Azabuensis : A Ruminal Bacterium That Produces Trans-11 Intermediates from Linoleic and Linolenic Acid.” MICROBIOLOGY-SGM, vol. 165, no. 7, 2019, pp. 772–78.
- APA
- Dewanckele, L., Vlaeminck, B., & Fievez, V. (2019). Sharpea azabuensis : a ruminal bacterium that produces trans-11 intermediates from linoleic and linolenic acid. MICROBIOLOGY-SGM, 165(7), 772–778.
- Chicago author-date
- Dewanckele, Lore, Bruno Vlaeminck, and Veerle Fievez. 2019. “Sharpea Azabuensis : A Ruminal Bacterium That Produces Trans-11 Intermediates from Linoleic and Linolenic Acid.” MICROBIOLOGY-SGM 165 (7): 772–78.
- Chicago author-date (all authors)
- Dewanckele, Lore, Bruno Vlaeminck, and Veerle Fievez. 2019. “Sharpea Azabuensis : A Ruminal Bacterium That Produces Trans-11 Intermediates from Linoleic and Linolenic Acid.” MICROBIOLOGY-SGM 165 (7): 772–778.
- Vancouver
- 1.Dewanckele L, Vlaeminck B, Fievez V. Sharpea azabuensis : a ruminal bacterium that produces trans-11 intermediates from linoleic and linolenic acid. MICROBIOLOGY-SGM. 2019;165(7):772–8.
- IEEE
- [1]L. Dewanckele, B. Vlaeminck, and V. Fievez, “Sharpea azabuensis : a ruminal bacterium that produces trans-11 intermediates from linoleic and linolenic acid,” MICROBIOLOGY-SGM, vol. 165, no. 7, pp. 772–778, 2019.
@article{8627614,
abstract = {To investigate the metabolism of 18: 2n-6 and 18: 3n-3 by pure cultures of Sharpea azabuensis, two different strains ( RL 1 and ST18) were each incubated in the presence of 40 mu g ml(-1) 18: 2n-6 or 18: 3n-3. Pure cultures of Butyrivibrio fibrisolvens D1 and Butyrivibrio proteoclasticus P18 were included as control treatments. Similar to the metabolism of B. fibrisolvens, both S. azabuensis strains converted 18: 2n-6 or 18: 3n-3 to cis-9, trans-11 CLA or cis-9, trans-11, cis-15 CLnA, after which it was further reduced to trans-11 18: 1 or trans-11, cis-15 18: 2, respectively. B. proteoclasticus additionally reduced trans-11 18: 1 to 18: 0. Trans-11, cis-15 18: 2 was also further metabolized by B. proteoclasticus, although trans-11 18: 1 did not accumulate, and only minor amounts of 18: 0 were formed. The time frame of 18: 2n-6 and 18: 3n-3 biohydrogenation by S. azabuensis was comparable with B. fibrisolvens, indicating that S. azabuensis and B. fibrisolvens might be alternative biohydrogenators of 18: 2n-6 and 18: 3n-3 in the rumen.},
author = {Dewanckele, Lore and Vlaeminck, Bruno and Fievez, Veerle},
issn = {1350-0872},
journal = {MICROBIOLOGY-SGM},
keywords = {biohydrogenation,linoleic acid,linolenic acid,Sharpea azabuensis,FATTY-ACIDS,COMMUNITY COMPOSITION,BIOHYDROGENATION,METABOLISM,ISOMERS,RUMEN},
language = {eng},
number = {7},
pages = {772--778},
title = {Sharpea azabuensis : a ruminal bacterium that produces trans-11 intermediates from linoleic and linolenic acid},
url = {http://dx.doi.org/10.1099/mic.0.000811},
volume = {165},
year = {2019},
}
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