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A specialized metabolic network selectively modulates Arabidopsis root microbiota

(2019) SCIENCE. 364(6440).
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Abstract
Plant specialized metabolites have ecological functions, yet the presence of numerous uncharacterized biosynthetic genes in plant genomes suggests that many molecules remain unknown. We discovered a triterpene biosynthetic network in the roots of the small mustard plant Arabidopsis thaliana. Collectively, we have elucidated and reconstituted three divergent pathways for the biosynthesis of root triterpenes, namely thalianin (seven steps), thalianyl medium-chain fatty acid esters (three steps), and arabidin (five steps). A. thaliana mutants disrupted in the biosynthesis of these compounds have altered root microbiota. In vitro bioassays with purified compounds reveal selective growth modulation activities of pathway metabolites toward root microbiota members and their biochemical transformation and utilization by bacteria, supporting a role for this biosynthetic network in shaping an Arabidopsis-specific root microbial community.
Keywords
OXIDOSQUALENE CYCLASE, GENE CLUSTERS, BIOSYNTHESIS, TRITERPENE, DIVERSIFICATION, MECHANISMS, EVOLUTION, PLANTS

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Citation

Please use this url to cite or link to this publication:

MLA
Huang, Ancheng C., et al. “A Specialized Metabolic Network Selectively Modulates Arabidopsis Root Microbiota.” SCIENCE, vol. 364, no. 6440, 2019.
APA
Huang, A. C., Jiang, T., Liu, Y.-X., Bai, Y., Reed, J., Qu, B., … Osbourn, A. (2019). A specialized metabolic network selectively modulates Arabidopsis root microbiota. SCIENCE, 364(6440).
Chicago author-date
Huang, Ancheng C, Ting Jiang, Yong-Xin Liu, Yuechen Bai, James Reed, Baoyuan Qu, Alain Goossens, Hans-Wilhelm Nützmann, Yang Bai, and Anne Osbourn. 2019. “A Specialized Metabolic Network Selectively Modulates Arabidopsis Root Microbiota.” SCIENCE 364 (6440).
Chicago author-date (all authors)
Huang, Ancheng C, Ting Jiang, Yong-Xin Liu, Yuechen Bai, James Reed, Baoyuan Qu, Alain Goossens, Hans-Wilhelm Nützmann, Yang Bai, and Anne Osbourn. 2019. “A Specialized Metabolic Network Selectively Modulates Arabidopsis Root Microbiota.” SCIENCE 364 (6440).
Vancouver
1.
Huang AC, Jiang T, Liu Y-X, Bai Y, Reed J, Qu B, et al. A specialized metabolic network selectively modulates Arabidopsis root microbiota. SCIENCE. 2019;364(6440).
IEEE
[1]
A. C. Huang et al., “A specialized metabolic network selectively modulates Arabidopsis root microbiota,” SCIENCE, vol. 364, no. 6440, 2019.
@article{8627401,
  abstract     = {Plant specialized metabolites have ecological functions, yet the presence of numerous uncharacterized biosynthetic genes in plant genomes suggests that many molecules remain unknown. We discovered a triterpene biosynthetic network in the roots of the small mustard plant Arabidopsis thaliana. Collectively, we have elucidated and reconstituted three divergent pathways for the biosynthesis of root triterpenes, namely thalianin (seven steps), thalianyl medium-chain fatty acid esters (three steps), and arabidin (five steps). A. thaliana mutants disrupted in the biosynthesis of these compounds have altered root microbiota. In vitro bioassays with purified compounds reveal selective growth modulation activities of pathway metabolites toward root microbiota members and their biochemical transformation and utilization by bacteria, supporting a role for this biosynthetic network in shaping an Arabidopsis-specific root microbial community.},
  articleno    = {eaau6389},
  author       = {Huang, Ancheng C and Jiang, Ting and Liu, Yong-Xin and Bai, Yuechen and Reed, James and Qu, Baoyuan and Goossens, Alain and Nützmann, Hans-Wilhelm and Bai, Yang and Osbourn, Anne},
  issn         = {0036-8075},
  journal      = {SCIENCE},
  keywords     = {OXIDOSQUALENE CYCLASE,GENE CLUSTERS,BIOSYNTHESIS,TRITERPENE,DIVERSIFICATION,MECHANISMS,EVOLUTION,PLANTS},
  language     = {eng},
  number       = {6440},
  pages        = {9},
  title        = {A specialized metabolic network selectively modulates Arabidopsis root microbiota},
  url          = {http://dx.doi.org/10.1126/science.aau6389},
  volume       = {364},
  year         = {2019},
}

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