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Portable multiplex immunochromatographic assay for quantitation of two typical algae toxins based on dual-color fluorescence microspheres

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Abstract
A multiplex immunochromatographic assay (ICA) based on dual-color fluorescent microspheres (FMs) as a sensitive label was developed for the first time. Two typical algae toxins, microcystin-LR (MC-LR) and okadaic acid (OA), were chosen as proof-of concept targets to evaluate the feasibility of this ICA format. Commercial red- and green-colored FMs were selected to couple with monoclonal antibodies as fluorescent probes. The use of dual-wavelength FMs as labels guaranteed a lower consumption of material strips, lower sample volume, and shorter reaction time without increasing the length of ICA strips. Under optimal conditions, the multiplex FM-ICA could be completed in 20 min and reached limits of detection for the simultaneous determination of MC-LR and OA in fish samples, which were 0.074 and 2.42 mu g/kg, respectively. The developed technique was validated using artificially spiked and naturally contaminated fish samples. Ultra-high-performance liquid chromatography-tandem mass spectrometry was used as confirmatory technique. In summary, this portable ICAs detection mode based on dual-wavelength FMs provided a reliable and sensitive on-site detection of multiple contaminants in food samples, which opens a new field for application of FMs in food safety.
Keywords
dual-color fluorescent microspheres, immunochromatographic assay, multiplex detection, portable, algae toxins, OKADAIC ACID, GOLD NANOPARTICLES, QUANTUM DOTS, IMMUNOASSAY, SPECIFICITY, ANTIBODIES, MICROCYSTINS, NODULARIN, ANALOGS, WATER

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Chicago
Zhang, Huiyan, Jiaxun Luo, Natalia Beloglazova, Shupeng Yang, Sarah De Saeger, Ghulam Mujtaba Mari, Suxia Zhang, Jianzhong Shen, Zhanhui Wang, and Xuezhi Yu. 2019. “Portable Multiplex Immunochromatographic Assay for Quantitation of Two Typical Algae Toxins Based on Dual-color Fluorescence Microspheres.” Journal of Agricultural and Food Chemistry 67 (21): 6041–6047.
APA
Zhang, Huiyan, Luo, J., Beloglazova, N., Yang, S., De Saeger, S., Mari, G. M., Zhang, S., et al. (2019). Portable multiplex immunochromatographic assay for quantitation of two typical algae toxins based on dual-color fluorescence microspheres. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 67(21), 6041–6047.
Vancouver
1.
Zhang H, Luo J, Beloglazova N, Yang S, De Saeger S, Mari GM, et al. Portable multiplex immunochromatographic assay for quantitation of two typical algae toxins based on dual-color fluorescence microspheres. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2019;67(21):6041–7.
MLA
Zhang, Huiyan et al. “Portable Multiplex Immunochromatographic Assay for Quantitation of Two Typical Algae Toxins Based on Dual-color Fluorescence Microspheres.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.21 (2019): 6041–6047. Print.
@article{8627291,
  abstract     = {A multiplex immunochromatographic assay (ICA) based on dual-color fluorescent microspheres (FMs) as a sensitive label was developed for the first time. Two typical algae toxins, microcystin-LR (MC-LR) and okadaic acid (OA), were chosen as proof-of concept targets to evaluate the feasibility of this ICA format. Commercial red- and green-colored FMs were selected to couple with monoclonal antibodies as fluorescent probes. The use of dual-wavelength FMs as labels guaranteed a lower consumption of material strips, lower sample volume, and shorter reaction time without increasing the length of ICA strips. Under optimal conditions, the multiplex FM-ICA could be completed in 20 min and reached limits of detection for the simultaneous determination of MC-LR and OA in fish samples, which were 0.074 and 2.42 mu g/kg, respectively. The developed technique was validated using artificially spiked and naturally contaminated fish samples. Ultra-high-performance liquid chromatography-tandem mass spectrometry was used as confirmatory technique. In summary, this portable ICAs detection mode based on dual-wavelength FMs provided a reliable and sensitive on-site detection of multiple contaminants in food samples, which opens a new field for application of FMs in food safety.},
  author       = {Zhang, Huiyan and Luo, Jiaxun and Beloglazova, Natalia and Yang, Shupeng and De Saeger, Sarah and Mari, Ghulam Mujtaba and Zhang, Suxia and Shen, Jianzhong and Wang, Zhanhui and Yu, Xuezhi},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  keywords     = {dual-color fluorescent microspheres,immunochromatographic assay,multiplex detection,portable,algae toxins,OKADAIC ACID,GOLD NANOPARTICLES,QUANTUM DOTS,IMMUNOASSAY,SPECIFICITY,ANTIBODIES,MICROCYSTINS,NODULARIN,ANALOGS,WATER},
  language     = {eng},
  number       = {21},
  pages        = {6041--6047},
  title        = {Portable multiplex immunochromatographic assay for quantitation of two typical algae toxins based on dual-color fluorescence microspheres},
  url          = {http://dx.doi.org/10.1021/acs.jafc.9b00011},
  volume       = {67},
  year         = {2019},
}

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