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Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone

(2019) TALANTA. 191. p.202-208
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Abstract
A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCI, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent to 1.9 pg mL(-1). It also demonstrated a good inter-assay precision (RSD < 10%). However, the tyramine-based capacitive immunosensor showed a bad repeatability (only 4 regeneration-interaction cycles were possible) and inter-assay precision (RSD > 15%) which did not allow sensitive and precise measurements. The LA-based method was compared with a direct ELISA. These results demonstrated that the label-free developed capacitive immunosensor had a better sensitivity and shorter analysis time in comparison with the direct microwell-plate format.
Keywords
Capacitive immunosensor, Folytyrarnine, Self-assembled monolayers, Zearalenone, Mycotoxin, LABEL-FREE IMMUNOSENSOR, SENSITIVE DETECTION, ELECTROCHEMICAL IMMUNOSENSOR, AFLATOXIN B-1, QUANTUM-DOTS, ULTRASENSITIVE DETECTION, GOLD ELECTRODE, IMPEDIMETRIC APTASENSOR, MYCOTOXIN ZEARALENONE, MASS-SPECTROMETRY

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Citation

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MLA
Foubert, Astrid et al. “Antibody Immobilization Strategy for the Development of a Capacitive Immunosensor Detecting Zearalenone.” TALANTA 191 (2019): 202–208. Print.
APA
Foubert, Astrid, Beloglazova, N., Hedstrom, M., & De Saeger, S. (2019). Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone. TALANTA, 191, 202–208.
Chicago author-date
Foubert, Astrid, Natalia Beloglazova, Martin Hedstrom, and Sarah De Saeger. 2019. “Antibody Immobilization Strategy for the Development of a Capacitive Immunosensor Detecting Zearalenone.” Talanta 191: 202–208.
Chicago author-date (all authors)
Foubert, Astrid, Natalia Beloglazova, Martin Hedstrom, and Sarah De Saeger. 2019. “Antibody Immobilization Strategy for the Development of a Capacitive Immunosensor Detecting Zearalenone.” Talanta 191: 202–208.
Vancouver
1.
Foubert A, Beloglazova N, Hedstrom M, De Saeger S. Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone. TALANTA. 2019;191:202–8.
IEEE
[1]
A. Foubert, N. Beloglazova, M. Hedstrom, and S. De Saeger, “Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone,” TALANTA, vol. 191, pp. 202–208, 2019.
@article{8627288,
  abstract     = {A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCI, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent to 1.9 pg mL(-1). It also demonstrated a good inter-assay precision (RSD < 10%). However, the tyramine-based capacitive immunosensor showed a bad repeatability (only 4 regeneration-interaction cycles were possible) and inter-assay precision (RSD > 15%) which did not allow sensitive and precise measurements. The LA-based method was compared with a direct ELISA. These results demonstrated that the label-free developed capacitive immunosensor had a better sensitivity and shorter analysis time in comparison with the direct microwell-plate format.},
  author       = {Foubert, Astrid and Beloglazova, Natalia and Hedstrom, Martin and De Saeger, Sarah},
  issn         = {0039-9140},
  journal      = {TALANTA},
  keywords     = {Capacitive immunosensor,Folytyrarnine,Self-assembled monolayers,Zearalenone,Mycotoxin,LABEL-FREE IMMUNOSENSOR,SENSITIVE DETECTION,ELECTROCHEMICAL IMMUNOSENSOR,AFLATOXIN B-1,QUANTUM-DOTS,ULTRASENSITIVE DETECTION,GOLD ELECTRODE,IMPEDIMETRIC APTASENSOR,MYCOTOXIN ZEARALENONE,MASS-SPECTROMETRY},
  language     = {eng},
  pages        = {202--208},
  title        = {Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone},
  url          = {http://dx.doi.org/10.1016/j.talanta.2018.08.062},
  volume       = {191},
  year         = {2019},
}

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