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Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone

Natalia Beloglazova (UGent) , Pieterjan Lenain (UGent) , Mickaël Tessier (UGent) , Irina Goryacheva, Zeger Hens (UGent) and Sarah De Saeger (UGent)
(2019) TALANTA. 192. p.169-174
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Abstract
A sensitive tool for simultaneous quantitative determination of two analytes in a single spot with the use of a bioimprinted protein is presented for the first time. BSA is chosen as a scaffold for generation of binding sites specific towards two compounds. A multiplex immunosorbent assay for screening of two cereal-born mycotoxins, deoxynivalenol and zearalenone, in wheat and maize is realized with the use of fluorescent silica coated quantum dots as labels. Water-soluble fluorescent nanostructures consist of core/shell Cd-QDs enrobed in silica shells to ensure their solubility. The mycotoxins are simultaneously detected by scanning the assay outcome at two different wavelengths, since two QD@SiO2 labelled conjugates fluorescent in different parts of the spectrum. The assay is combined with a rapid extraction technique. The limits of detection for the simultaneous determination were 100 and 700 mu g kg(-1) in both matrices for zearalenone and deoxynivalenol, respectively. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the obtained results.
Keywords
Bioimprinting, Quantum dots, Silica coated quantum dot, Immunoassay, Zearalenone, Deoxynivalenol, MOLECULARLY IMPRINTED POLYMERS, MULTICOLOR QUANTUM DOTS, SOLID-PHASE EXTRACTION, AFLATOXIN B-1, OCHRATOXIN-A, IMMUNOASSAY, SAMPLES, LABELS, COOCCURRENCE, ANTIBIOTICS

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Citation

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MLA
Beloglazova, Natalia et al. “Bioimprinting for Multiplex Luminescent Detection of Deoxynivalenol and Zearalenone.” TALANTA 192 (2019): 169–174. Print.
APA
Beloglazova, Natalia, Lenain, P., Tessier, M., Goryacheva, I., Hens, Z., & De Saeger, S. (2019). Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone. TALANTA, 192, 169–174.
Chicago author-date
Beloglazova, Natalia, Pieterjan Lenain, Mickaël Tessier, Irina Goryacheva, Zeger Hens, and Sarah De Saeger. 2019. “Bioimprinting for Multiplex Luminescent Detection of Deoxynivalenol and Zearalenone.” Talanta 192: 169–174.
Chicago author-date (all authors)
Beloglazova, Natalia, Pieterjan Lenain, Mickaël Tessier, Irina Goryacheva, Zeger Hens, and Sarah De Saeger. 2019. “Bioimprinting for Multiplex Luminescent Detection of Deoxynivalenol and Zearalenone.” Talanta 192: 169–174.
Vancouver
1.
Beloglazova N, Lenain P, Tessier M, Goryacheva I, Hens Z, De Saeger S. Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone. TALANTA. 2019;192:169–74.
IEEE
[1]
N. Beloglazova, P. Lenain, M. Tessier, I. Goryacheva, Z. Hens, and S. De Saeger, “Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone,” TALANTA, vol. 192, pp. 169–174, 2019.
@article{8627286,
  abstract     = {A sensitive tool for simultaneous quantitative determination of two analytes in a single spot with the use of a bioimprinted protein is presented for the first time. BSA is chosen as a scaffold for generation of binding sites specific towards two compounds. A multiplex immunosorbent assay for screening of two cereal-born mycotoxins, deoxynivalenol and zearalenone, in wheat and maize is realized with the use of fluorescent silica coated quantum dots as labels. Water-soluble fluorescent nanostructures consist of core/shell Cd-QDs enrobed in silica shells to ensure their solubility. The mycotoxins are simultaneously detected by scanning the assay outcome at two different wavelengths, since two QD@SiO2 labelled conjugates fluorescent in different parts of the spectrum. The assay is combined with a rapid extraction technique. The limits of detection for the simultaneous determination were 100 and 700 mu g kg(-1) in both matrices for zearalenone and deoxynivalenol, respectively. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the obtained results.},
  author       = {Beloglazova, Natalia and Lenain, Pieterjan and Tessier, Mickaël and Goryacheva, Irina and Hens, Zeger and De Saeger, Sarah},
  issn         = {0039-9140},
  journal      = {TALANTA},
  keywords     = {Bioimprinting,Quantum dots,Silica coated quantum dot,Immunoassay,Zearalenone,Deoxynivalenol,MOLECULARLY IMPRINTED POLYMERS,MULTICOLOR QUANTUM DOTS,SOLID-PHASE EXTRACTION,AFLATOXIN B-1,OCHRATOXIN-A,IMMUNOASSAY,SAMPLES,LABELS,COOCCURRENCE,ANTIBIOTICS},
  language     = {eng},
  pages        = {169--174},
  title        = {Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone},
  url          = {http://dx.doi.org/10.1016/j.talanta.2018.09.042},
  volume       = {192},
  year         = {2019},
}
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