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Exploring light-sensitive nanocarriers for simultaneous triggered antibiotic release and disruption of biofilms upon generation of laser-induced vapor nanobubbles

(2019) PHARMACEUTICS. 11(5).
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Abstract
Impaired penetration of antibiotics through bacterial biofilms is one of the reasons for failure of antimicrobial therapy. Hindered drug diffusion is caused on the one hand by interactions with the sticky biofilm matrix and on the other hand by the fact that bacterial cells are organized in densely packed clusters of cells. Binding interactions with the biofilm matrix can be avoided by encapsulating the antibiotics into nanocarriers, while interfering with the integrity of the dense cell clusters can enhance drug transport deep into the biofilm. Vapor nanobubbles (VNB), generated from laser irradiated nanoparticles, are a recently reported effective way to loosen up the biofilm structure in order to enhance drug transport and efficacy. In the present study, we explored if the disruptive force of VNB can be used simultaneously to interfere with the biofilm structure and trigger antibiotic release from light-responsive nanocarriers. The antibiotic tobramycin was incorporated in two types of light-responsive nanocarriersliposomes functionalized with gold nanoparticles (Lip-AuNP) and graphene quantum dots (GQD)and their efficacy was evaluated on Pseudomonas aeruginosa biofilms. Even though the anti-biofilm efficacy of tobramycin was improved by liposomal encapsulation, electrostatic functionalization with 70 nm AuNP unfortunately resulted in premature leakage of tobramycin in a matter of hours. Laser-irradiation consequently did not further improve P. aeruginosa biofilm eradication. Adsorption of tobramycin to GQD, on the other hand, did result in a stable formulation with high encapsulation efficiency, without burst release of tobramycin from the nanocarriers. However, even though laser-induced VNB formation from GQD resulted in biofilm disruption, an enhanced anti-biofilm effect was not achieved due to tobramycin not being efficiently released from GQD. Even though this study was unsuccessful in designing suitable nanocarriers for simultaneous biofilm disruption and light-triggered release of tobramycin, it provides insights into the difficulties and challenges that need to be considered for future developments in this regard.
Keywords
DEHYDRATION-REHYDRATION VESICLES, REDUCED GRAPHENE OXIDE, DRUG-DELIVERY, BACTERIAL, NANOPARTICLES, PENETRATION, INSULIN, vapor nanobubbles, laser treatment, triggered release, liposomes, gold, nanoparticles, graphene quantum dots, biofilms, diffusion barrier

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MLA
Teirlinck, Eline et al. “Exploring Light-sensitive Nanocarriers for Simultaneous Triggered Antibiotic Release and Disruption of Biofilms Upon Generation of Laser-induced Vapor Nanobubbles.” PHARMACEUTICS 11.5 (2019): n. pag. Print.
APA
Teirlinck, E., Barras, A., Liu, J., Fraire, J., Lajunen, T., Xiong, R., Forier, K., et al. (2019). Exploring light-sensitive nanocarriers for simultaneous triggered antibiotic release and disruption of biofilms upon generation of laser-induced vapor nanobubbles. PHARMACEUTICS, 11(5).
Chicago author-date
Teirlinck, Eline, Alexandre Barras, Jing Liu, Juan Fraire, Tatu Lajunen, Ranhua Xiong, Katrien Forier, et al. 2019. “Exploring Light-sensitive Nanocarriers for Simultaneous Triggered Antibiotic Release and Disruption of Biofilms Upon Generation of Laser-induced Vapor Nanobubbles.” Pharmaceutics 11 (5).
Chicago author-date (all authors)
Teirlinck, Eline, Alexandre Barras, Jing Liu, Juan Fraire, Tatu Lajunen, Ranhua Xiong, Katrien Forier, Chengnan Li, Arto Urtti, Rabah Boukherroub, Sabine Szunerits, Stefaan De Smedt, Tom Coenye, and Kevin Braeckmans. 2019. “Exploring Light-sensitive Nanocarriers for Simultaneous Triggered Antibiotic Release and Disruption of Biofilms Upon Generation of Laser-induced Vapor Nanobubbles.” Pharmaceutics 11 (5).
Vancouver
1.
Teirlinck E, Barras A, Liu J, Fraire J, Lajunen T, Xiong R, et al. Exploring light-sensitive nanocarriers for simultaneous triggered antibiotic release and disruption of biofilms upon generation of laser-induced vapor nanobubbles. PHARMACEUTICS. 2019;11(5).
IEEE
[1]
E. Teirlinck et al., “Exploring light-sensitive nanocarriers for simultaneous triggered antibiotic release and disruption of biofilms upon generation of laser-induced vapor nanobubbles,” PHARMACEUTICS, vol. 11, no. 5, 2019.
@article{8626676,
  abstract     = {Impaired penetration of antibiotics through bacterial biofilms is one of the reasons for failure of antimicrobial therapy. Hindered drug diffusion is caused on the one hand by interactions with the sticky biofilm matrix and on the other hand by the fact that bacterial cells are organized in densely packed clusters of cells. Binding interactions with the biofilm matrix can be avoided by encapsulating the antibiotics into nanocarriers, while interfering with the integrity of the dense cell clusters can enhance drug transport deep into the biofilm. Vapor nanobubbles (VNB), generated from laser irradiated nanoparticles, are a recently reported effective way to loosen up the biofilm structure in order to enhance drug transport and efficacy. In the present study, we explored if the disruptive force of VNB can be used simultaneously to interfere with the biofilm structure and trigger antibiotic release from light-responsive nanocarriers. The antibiotic tobramycin was incorporated in two types of light-responsive nanocarriersliposomes functionalized with gold nanoparticles (Lip-AuNP) and graphene quantum dots (GQD)and their efficacy was evaluated on Pseudomonas aeruginosa biofilms. Even though the anti-biofilm efficacy of tobramycin was improved by liposomal encapsulation, electrostatic functionalization with 70 nm AuNP unfortunately resulted in premature leakage of tobramycin in a matter of hours. Laser-irradiation consequently did not further improve P. aeruginosa biofilm eradication. Adsorption of tobramycin to GQD, on the other hand, did result in a stable formulation with high encapsulation efficiency, without burst release of tobramycin from the nanocarriers. However, even though laser-induced VNB formation from GQD resulted in biofilm disruption, an enhanced anti-biofilm effect was not achieved due to tobramycin not being efficiently released from GQD. Even though this study was unsuccessful in designing suitable nanocarriers for simultaneous biofilm disruption and light-triggered release of tobramycin, it provides insights into the difficulties and challenges that need to be considered for future developments in this regard.},
  articleno    = {201},
  author       = {Teirlinck, Eline and Barras, Alexandre and Liu, Jing and Fraire, Juan and Lajunen, Tatu and Xiong, Ranhua and Forier, Katrien and Li, Chengnan and Urtti, Arto and Boukherroub, Rabah and Szunerits, Sabine and De Smedt, Stefaan and Coenye, Tom and Braeckmans, Kevin},
  issn         = {1999-4923},
  journal      = {PHARMACEUTICS},
  keywords     = {DEHYDRATION-REHYDRATION VESICLES,REDUCED GRAPHENE OXIDE,DRUG-DELIVERY,BACTERIAL,NANOPARTICLES,PENETRATION,INSULIN,vapor nanobubbles,laser treatment,triggered release,liposomes,gold,nanoparticles,graphene quantum dots,biofilms,diffusion barrier},
  language     = {eng},
  number       = {5},
  pages        = {15},
  title        = {Exploring light-sensitive nanocarriers for simultaneous triggered antibiotic release and disruption of biofilms upon generation of laser-induced vapor nanobubbles},
  url          = {http://dx.doi.org/10.3390/pharmaceutics11050201},
  volume       = {11},
  year         = {2019},
}

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