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Cichorium intybus L. × Cicerbita alpina Walbr. : doubled haploid chicory induction and CENH3 characterization

Jeroen Van der Veken, Tom Eeckhaut, Joost Baert, Tom Ruttink (UGent) , Olivier Maudoux, Stefaan Werbrouck (UGent) and Johan Van Huylenbroeck
(2019) EUPHYTICA. 215(7).
Author
Organization
Abstract
Intergeneric hybridization between industrial chicory (Cichorium intybus L.) and Cicerbita alpina Walbr. induces interspecific hybrids and haploid chicory plants after in vitro embryo rescue. The protocol yielded haploids in 5 out of 12 cultivars pollinated; altogether 18 haploids were regenerated from 2836 embryos, with a maximum efficiency of 1.96% haploids per cross. Obtained haploids were chromosome doubled with mitosis inhibitors trifluralin and oryzalin; exposure to 0.05gL(-1) oryzalin during one week was the most efficient treatment to regenerate doubled haploids. Inbreeding effects in vitro were limited, but the ploidy level affects morphology. Transcriptome sequencing revealed two unique copies of CENH3 in Cicerbita alpina Walbr. Comparison of CENH3.1 protein sequences of Cicerbita and Cichorium obtained through transcriptome and whole shotgun genome sequencing revealed two amino-acid substitutions at critical residues of the histone fold domain. These particular changes cause chromosome elimination and reduced centromere loading in several other species and might indicate a CENH3-dependent mechanism causing chromosome elimination of parental chromosomes during CichoriumxCicerbita intergeneric hybridization. Our results provide insights in chromosome elimination and might increase the efficiency of haploid induction in Cichorium.
Keywords
Chromosome elimination, Intergeneric hybridization, Chromosome doubling, Industrial chicory, CENH3, CHROMOSOME ELIMINATION, IMMATURE EMBRYOS, PLANTS, INCOMPATIBILITY, TEMPERATURE, LINES, L.

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MLA
Van der Veken, Jeroen, et al. “Cichorium Intybus L. × Cicerbita Alpina Walbr. : Doubled Haploid Chicory Induction and CENH3 Characterization.” EUPHYTICA, vol. 215, no. 7, 2019, doi:10.1007/s10681-019-2435-0.
APA
Van der Veken, J., Eeckhaut, T., Baert, J., Ruttink, T., Maudoux, O., Werbrouck, S., & Van Huylenbroeck, J. (2019). Cichorium intybus L. × Cicerbita alpina Walbr. : doubled haploid chicory induction and CENH3 characterization. EUPHYTICA, 215(7). https://doi.org/10.1007/s10681-019-2435-0
Chicago author-date
Van der Veken, Jeroen, Tom Eeckhaut, Joost Baert, Tom Ruttink, Olivier Maudoux, Stefaan Werbrouck, and Johan Van Huylenbroeck. 2019. “Cichorium Intybus L. × Cicerbita Alpina Walbr. : Doubled Haploid Chicory Induction and CENH3 Characterization.” EUPHYTICA 215 (7). https://doi.org/10.1007/s10681-019-2435-0.
Chicago author-date (all authors)
Van der Veken, Jeroen, Tom Eeckhaut, Joost Baert, Tom Ruttink, Olivier Maudoux, Stefaan Werbrouck, and Johan Van Huylenbroeck. 2019. “Cichorium Intybus L. × Cicerbita Alpina Walbr. : Doubled Haploid Chicory Induction and CENH3 Characterization.” EUPHYTICA 215 (7). doi:10.1007/s10681-019-2435-0.
Vancouver
1.
Van der Veken J, Eeckhaut T, Baert J, Ruttink T, Maudoux O, Werbrouck S, et al. Cichorium intybus L. × Cicerbita alpina Walbr. : doubled haploid chicory induction and CENH3 characterization. EUPHYTICA. 2019;215(7).
IEEE
[1]
J. Van der Veken et al., “Cichorium intybus L. × Cicerbita alpina Walbr. : doubled haploid chicory induction and CENH3 characterization,” EUPHYTICA, vol. 215, no. 7, 2019.
@article{8626061,
  abstract     = {{Intergeneric hybridization between industrial chicory (Cichorium intybus L.) and Cicerbita alpina Walbr. induces interspecific hybrids and haploid chicory plants after in vitro embryo rescue. The protocol yielded haploids in 5 out of 12 cultivars pollinated; altogether 18 haploids were regenerated from 2836 embryos, with a maximum efficiency of 1.96% haploids per cross. Obtained haploids were chromosome doubled with mitosis inhibitors trifluralin and oryzalin; exposure to 0.05gL(-1) oryzalin during one week was the most efficient treatment to regenerate doubled haploids. Inbreeding effects in vitro were limited, but the ploidy level affects morphology. Transcriptome sequencing revealed two unique copies of CENH3 in Cicerbita alpina Walbr. Comparison of CENH3.1 protein sequences of Cicerbita and Cichorium obtained through transcriptome and whole shotgun genome sequencing revealed two amino-acid substitutions at critical residues of the histone fold domain. These particular changes cause chromosome elimination and reduced centromere loading in several other species and might indicate a CENH3-dependent mechanism causing chromosome elimination of parental chromosomes during CichoriumxCicerbita intergeneric hybridization. Our results provide insights in chromosome elimination and might increase the efficiency of haploid induction in Cichorium.}},
  articleno    = {{134}},
  author       = {{Van der Veken, Jeroen and Eeckhaut, Tom and Baert, Joost and Ruttink, Tom and Maudoux, Olivier and Werbrouck, Stefaan and Van Huylenbroeck, Johan}},
  issn         = {{0014-2336}},
  journal      = {{EUPHYTICA}},
  keywords     = {{Chromosome elimination,Intergeneric hybridization,Chromosome doubling,Industrial chicory,CENH3,CHROMOSOME ELIMINATION,IMMATURE EMBRYOS,PLANTS,INCOMPATIBILITY,TEMPERATURE,LINES,L.}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{13}},
  title        = {{Cichorium intybus L. × Cicerbita alpina Walbr. : doubled haploid chicory induction and CENH3 characterization}},
  url          = {{http://doi.org/10.1007/s10681-019-2435-0}},
  volume       = {{215}},
  year         = {{2019}},
}
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