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Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells

(2019) IMMUNITY. 51(1). p.169-184
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Abstract
Naive CD4(+) T cells differentiate into functionally diverse T helper (Th) cell subsets. Th2 cells play a pathogenic role in asthma, yet a clear picture of their transcriptional profile is lacking. We performed single-cell RNA sequencing (scRNA-seq) of T helper cells from lymph node, lung, and airways in the house dust mite (HDM) model of allergic airway disease. scRNA-seq resolved transcriptional profiles of naive CD4(+) T, Th1, Th2, regulatory T (Treg) cells, and a CD4(+) T cell population responsive to type I interferons. Th2 cells in the airways were enriched for transcription of many genes, including Cd200r1, Il6, Plac8, and Igfbp7, and their mRNA profile was supported by analysis of chromatin accessibility and flow cytometry. Pathways associated with lipid metabolism were enriched in Th2 cells, and experiments with inhibitors of key metabolic pathways supported roles for glucose and lipid metabolism. These findings provide insight into the differentiation of pathogenic Th2 cells in the context of allergy.
Keywords
INNATE LYMPHOID-CELLS, CO-STIMULATION, PPAR-GAMMA, DIFFERENTIATION, ASTHMA, MECHANISMS, DEFICIENCY, ACTIVATION, IMMUNITY

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Citation

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Chicago
Tibbitt, Christopher Andrew, Julian Mario Stark, Liesbet Martens, Junjie Ma, Jeff Eron Mold, Kim Deswarte, Ganna Oliynyk, et al. 2019. “Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells.” Immunity 51 (1): 169–184.
APA
Tibbitt, C. A., Stark, J. M., Martens, L., Ma, J., Mold, J. E., Deswarte, K., Oliynyk, G., et al. (2019). Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells. IMMUNITY, 51(1), 169–184.
Vancouver
1.
Tibbitt CA, Stark JM, Martens L, Ma J, Mold JE, Deswarte K, et al. Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells. IMMUNITY. Cambridge: Cell Press; 2019;51(1):169–84.
MLA
Tibbitt, Christopher Andrew et al. “Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells.” IMMUNITY 51.1 (2019): 169–184. Print.
@article{8625734,
  abstract     = {Naive CD4(+) T cells differentiate into functionally diverse T helper (Th) cell subsets. Th2 cells play a pathogenic role in asthma, yet a clear picture of their transcriptional profile is lacking. We performed single-cell RNA sequencing (scRNA-seq) of T helper cells from lymph node, lung, and airways in the house dust mite (HDM) model of allergic airway disease. scRNA-seq resolved transcriptional profiles of naive CD4(+) T, Th1, Th2, regulatory T (Treg) cells, and a CD4(+) T cell population responsive to type I interferons. Th2 cells in the airways were enriched for transcription of many genes, including Cd200r1, Il6, Plac8, and Igfbp7, and their mRNA profile was supported by analysis of chromatin accessibility and flow cytometry. Pathways associated with lipid metabolism were enriched in Th2 cells, and experiments with inhibitors of key metabolic pathways supported roles for glucose and lipid metabolism. These findings provide insight into the differentiation of pathogenic Th2 cells in the context of allergy.},
  author       = {Tibbitt, Christopher Andrew and Stark, Julian Mario and Martens, Liesbet and Ma, Junjie and Mold, Jeff Eron and Deswarte, Kim and Oliynyk, Ganna and Feng, Xiaogang and Lambrecht, Bart and De Bleser, Pieter and Nylen, Susanne and Hammad, Hamida and Henriksson, Marie Arsenian and Saeys, Yvan and Coquet, Jonathan Marie},
  issn         = {1074-7613},
  journal      = {IMMUNITY},
  keywords     = {INNATE LYMPHOID-CELLS,CO-STIMULATION,PPAR-GAMMA,DIFFERENTIATION,ASTHMA,MECHANISMS,DEFICIENCY,ACTIVATION,IMMUNITY},
  language     = {eng},
  number       = {1},
  pages        = {169--184},
  publisher    = {Cell Press},
  title        = {Single-Cell RNA Sequencing of the T Helper Cell Response to House Dust Mites Defines a Distinct Gene Expression Signature in Airway Th2 Cells},
  url          = {http://dx.doi.org/10.1016/j.immuni.2019.05.014},
  volume       = {51},
  year         = {2019},
}

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