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Protein N-glycosylation in the hemipteran pest insect Nilaparvata lugens

(2019)
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Abstract
N-glycosylation is one of the most abundant and conserved protein modifications in eukaryotes. In insects, protein N-glycosylation plays a vital role in growth, development and survival, which makes it an interesting target for future pest control strategies. So far, all research on protein N-glycosylation has been conducted in holometabolous (pest) insects, while there is no information available for hemimetabolous insects. To fill this gap in our knowledge, this thesis focuses on protein N-glycosylation in the hemimetabolous model insect Nilaparvata lugens. N. lugens, or the brown planthopper, is one of the most notorious pest insects of cultured rice, and outbreaks of this insect lead to enormous economic losses each year. When studying protein N-glycosylation in N. lugens, no dynamics were observed during post-embryonic development. In contrast, discrepancies in the N-glycomes of adult morphs were observed between adult sexes, rather than between wing types. The female adult develops a unique N-glycan fingerprint, with a distinct increase in high mannose N-glycans. In addition, enrichment of glycopeptides revealed both differential glycosylation between males and females of proteins occurring in both sexes, as well as sex-specific glycoproteins. All together these data provide novel insights into planthopper’s biology, confirm that protein N-glycosylation is sex-related in this insect, and offer novel targets for the control of N. lugens.
N-glycosylatie is één van de meest voorkomende en geconserveerde modificaties van eiwitten in eukaryoten. In insecten speelt dit proces een cruciale rol in hun groei, ontwikkeling en overleving, wat inhoudt dat dit proces een interessant doelwit kan zijn voor toekomstige strategieën voor het bestrijden van insecten. Onderzoek naar eiwit N-glycosylatie in insecten werd tot dusver enkel uitgevoerd in (plaag)insecten die een complete metamorfose ondergaan tijdens hun ontwikkeling, terwijl er geen informatie beschikbaar is voor hemimetabole insecten ofwel insecten die een incomplete gedaanteverwisseling ondergaan. Om dit gebrek aan informatie aan te vullen, focust deze thesis zich op eiwit N-glycosylatie in het hemimetabool plaaginsect Nilaparvata lugens. N. lugens, of de bruine dwergcicade, is één van de meest schadelijke plaaginsecten van rijstplanten, en een uitbraak van dit insect kan leiden tot enorme economische verliezen. Tijdens het bestuderen van eiwit N-glycosylatie in N. lugens werd duidelijk dat er geen verschillen waarneembaar zijn tijdens de post-embryonale ontwikkeling. Echter, verschillen in N-glycomen werden geobserveerd voor adulte insecten, en traden op tussen mannelijke en vrouwelijke adulten, en niet tussen de vleugelvormen. Het vrouwelijke volwassen insect ontwikkelt een uniek N-glycaan profiel met een duidelijke toename in hoog mannose N-glycanen. Verder bleek ook na verrijking van glycopeptiden dat zowel verschillen in glycosylatie tussen mannelijke en vrouwelijke insecten voorkomen voor eiwitten aanwezig in beide geslachten, alsook dat geslachtsspecifieke glyco-eiwitten voorkomen. Samengevat, deze resultaten bieden nieuwe inzichten in de biologie van cicaden, bevestigen dat eiwit N-glycosylatie geslachtsafhankelijk is in dit insect, en bieden nieuwe doelwitten voor toekomstige bestrijding van N. lugens.
Keywords
Glycosylation, insect, N-glycosylation, pest insect, Nilaparvata lugens

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Citation

Please use this url to cite or link to this publication:

MLA
Scheys, Freja. Protein N-Glycosylation  in the Hemipteran Pest Insect Nilaparvata Lugens. Ghent University. Faculty of Bioscience Engineering, 2019.
APA
Scheys, F. (2019). Protein N-glycosylation  in the hemipteran pest insect Nilaparvata lugens. Ghent University. Faculty of Bioscience Engineering, Ghent, Belgium.
Chicago author-date
Scheys, Freja. 2019. “Protein N-Glycosylation  in the Hemipteran Pest Insect Nilaparvata Lugens.” Ghent, Belgium: Ghent University. Faculty of Bioscience Engineering.
Chicago author-date (all authors)
Scheys, Freja. 2019. “Protein N-Glycosylation  in the Hemipteran Pest Insect Nilaparvata Lugens.” Ghent, Belgium: Ghent University. Faculty of Bioscience Engineering.
Vancouver
1.
Scheys F. Protein N-glycosylation  in the hemipteran pest insect Nilaparvata lugens. [Ghent, Belgium]: Ghent University. Faculty of Bioscience Engineering; 2019.
IEEE
[1]
F. Scheys, “Protein N-glycosylation  in the hemipteran pest insect Nilaparvata lugens,” Ghent University. Faculty of Bioscience Engineering, Ghent, Belgium, 2019.
@phdthesis{8625658,
  abstract     = {{N-glycosylation is one of the most abundant and conserved protein modifications in eukaryotes. In insects, protein N-glycosylation plays a vital role in growth, development and survival, which makes it an interesting target for future pest control strategies. So far, all research on protein N-glycosylation has been conducted in holometabolous (pest) insects, while there is no information available for hemimetabolous insects. To fill this gap in our knowledge, this thesis focuses on protein N-glycosylation in the hemimetabolous model insect Nilaparvata lugens.
N. lugens, or the brown planthopper, is one of the most notorious pest insects of cultured rice, and outbreaks of this insect lead to enormous economic losses each year. When studying protein N-glycosylation in N. lugens, no dynamics were observed during post-embryonic development. In contrast, discrepancies in the N-glycomes of adult morphs were observed between adult sexes, rather than between wing types. The female adult develops a unique N-glycan fingerprint, with a distinct increase in high mannose N-glycans. In addition, enrichment of glycopeptides revealed both differential glycosylation between males and females of proteins occurring in both sexes, as well as sex-specific glycoproteins. 
All together these data provide novel insights into planthopper’s biology, confirm that protein N-glycosylation is sex-related in this insect, and offer novel targets for the control of N. lugens.}},
  author       = {{Scheys, Freja}},
  isbn         = {{9789463572316}},
  keywords     = {{Glycosylation,insect,N-glycosylation,pest insect,Nilaparvata lugens}},
  language     = {{eng}},
  pages        = {{VIII, 176}},
  publisher    = {{Ghent University. Faculty of Bioscience Engineering}},
  school       = {{Ghent University}},
  title        = {{Protein N-glycosylation  in the hemipteran pest insect Nilaparvata lugens}},
  year         = {{2019}},
}