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Miniaturization of Starmerella bombicola fermentation for evaluation and increasing (novel) glycolipid production

Author
Organization
Abstract
Both strain engineering and process optimization are intensively studied in microbial biosurfactant literature. However, screening of multiple strains and/or medium components in parallel is a very labor-intensive and timely process, considering the only applied technique nowadays is evaluation through shake flask and/or bioreactor experiments. Therefore, in this work, the development, optimization, and application of a more throughput techniquebased on 24-deep well platesare described for a new Starmerella bombicola strain producing bolaform sophorolipids. To develop an optimal setup, the influence of plate position and culture volume and the type of sandwich cover was investigated. Optimal parameters, which did not result in significant differences compared with shake flask experiments concerning growth, glucose consumption, and production of novel sophorolipids, were defined and validated. Next, the new method was applied to evaluate the influence of the use of alternative (commercial) nitrogen sources in comparison with the yeast extract currently applied in the production medium, aiming to increase production efficiency. Self-made yeast extracts from S. bombicola cells were also included to evaluate possible recycling of cells after fermentation. In conclusion, the designed method enabled the efficient and successful comparison of ten different nitrogen sources in varying concentrations (1, 4, and 10g/L) on bola sophorolipid production, which can now also be performed for other parameters important for growth and/or glycolipid production.
Keywords
Starmerella bombicola, Biosurfactant, Glycolipid, Throughput screening, Medium optimization, Nitrogen source, SOPHOROSE LIPID PRODUCTION, SPENT BREWERS-YEAST, CANDIDA-BOMBICOLA, MICROBIAL-PRODUCTION, MICROTITER PLATES, OXYGEN-TRANSFER, SOPHOROLIPIDS, GROWTH, BIOSYNTHESIS, OPTIMIZATION

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MLA
Van Renterghem, Lisa, et al. “Miniaturization of Starmerella Bombicola Fermentation for Evaluation and Increasing (Novel) Glycolipid Production.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 103, no. 11, 2019, pp. 4347–62.
APA
Van Renterghem, L., Clicque, H., Huyst, A., Roelants, S., & Soetaert, W. (2019). Miniaturization of Starmerella bombicola fermentation for evaluation and increasing (novel) glycolipid production. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 103(11), 4347–4362.
Chicago author-date
Van Renterghem, Lisa, Hadewijch Clicque, Arne Huyst, Sophie Roelants, and Wim Soetaert. 2019. “Miniaturization of Starmerella Bombicola Fermentation for Evaluation and Increasing (Novel) Glycolipid Production.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103 (11): 4347–62.
Chicago author-date (all authors)
Van Renterghem, Lisa, Hadewijch Clicque, Arne Huyst, Sophie Roelants, and Wim Soetaert. 2019. “Miniaturization of Starmerella Bombicola Fermentation for Evaluation and Increasing (Novel) Glycolipid Production.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103 (11): 4347–4362.
Vancouver
1.
Van Renterghem L, Clicque H, Huyst A, Roelants S, Soetaert W. Miniaturization of Starmerella bombicola fermentation for evaluation and increasing (novel) glycolipid production. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2019;103(11):4347–62.
IEEE
[1]
L. Van Renterghem, H. Clicque, A. Huyst, S. Roelants, and W. Soetaert, “Miniaturization of Starmerella bombicola fermentation for evaluation and increasing (novel) glycolipid production,” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 103, no. 11, pp. 4347–4362, 2019.
@article{8625320,
  abstract     = {Both strain engineering and process optimization are intensively studied in microbial biosurfactant literature. However, screening of multiple strains and/or medium components in parallel is a very labor-intensive and timely process, considering the only applied technique nowadays is evaluation through shake flask and/or bioreactor experiments. Therefore, in this work, the development, optimization, and application of a more throughput techniquebased on 24-deep well platesare described for a new Starmerella bombicola strain producing bolaform sophorolipids. To develop an optimal setup, the influence of plate position and culture volume and the type of sandwich cover was investigated. Optimal parameters, which did not result in significant differences compared with shake flask experiments concerning growth, glucose consumption, and production of novel sophorolipids, were defined and validated. Next, the new method was applied to evaluate the influence of the use of alternative (commercial) nitrogen sources in comparison with the yeast extract currently applied in the production medium, aiming to increase production efficiency. Self-made yeast extracts from S. bombicola cells were also included to evaluate possible recycling of cells after fermentation. In conclusion, the designed method enabled the efficient and successful comparison of ten different nitrogen sources in varying concentrations (1, 4, and 10g/L) on bola sophorolipid production, which can now also be performed for other parameters important for growth and/or glycolipid production.},
  author       = {Van Renterghem, Lisa and Clicque, Hadewijch and Huyst, Arne and Roelants, Sophie and Soetaert, Wim},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keywords     = {Starmerella bombicola,Biosurfactant,Glycolipid,Throughput screening,Medium optimization,Nitrogen source,SOPHOROSE LIPID PRODUCTION,SPENT BREWERS-YEAST,CANDIDA-BOMBICOLA,MICROBIAL-PRODUCTION,MICROTITER PLATES,OXYGEN-TRANSFER,SOPHOROLIPIDS,GROWTH,BIOSYNTHESIS,OPTIMIZATION},
  language     = {eng},
  number       = {11},
  pages        = {4347--4362},
  title        = {Miniaturization of Starmerella bombicola fermentation for evaluation and increasing (novel) glycolipid production},
  url          = {http://dx.doi.org/10.1007/s00253-019-09766-3},
  volume       = {103},
  year         = {2019},
}

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