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Hot-melt preparation of a non-biodegradable peptide implant : a proof of principle

(2019) PROTEIN AND PEPTIDE LETTERS. 26(9). p.691-701
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Abstract
Background: Both biodegradable and non-biodegradable peptide-loaded implants are already developed for the long-term treatment of patients, thereby reducing the frequency of drug administration. To further improve peptide formulation, extending the scope of implant-based drug delivery systems towards other polymers and processing techniques is highly interesting. Objective: In this study, as a proof-of-principle, the feasibility of hot-melt processing of a peptide active pharmaceutical ingredient was assessed by developing a non-biodegradable poly(ethylene- vinyl acetate) (33% VA) implant loaded with 20% (w/w) buserelin acetate. Methods: Cross-sectional implant characterization was performed by Raman microscopy. The stability of buserelin acetate in the polymeric matrix was evaluated for 3 months under ICH stability conditions and the quantity as well as the degradation products analyzed using LC-UV methods. An in vitro dissolution study was performed as well and buserelin acetate and its degradants analyzed using the same chromatographic methods. Results: No significant quantities of buserelin acetate-related degradation products were formed during the hot-melt preparation as well as during the stability study. Together with the consistent buserelin acetate assay values over time, chemical peptide stability was thus demonstrated. The in vitro buserelin acetate release from the implant was found to be diffusion-controlled after an initial burst release, with stable release profiles in the stability study, demonstrating the functional stability of the peptide implant. Conclusion: These results indicate the feasibility of preparing non-biodegradable peptide-loaded implants using the hot-melt production method and may act as a proof of principle concept for further innovation in peptide medicinal formulations.
Keywords
Buserelin acetate, hot-melt process, poly(ethylene-vinyl acetate) polymeric formulation, analytical characterization, dissolution, stability, ETHYLENE-VINYL ACETATE, GONADOTROPIN-RELEASING-HORMONE, DRUG-RELEASE, SUSTAINED-RELEASE, IN-VITRO, CLINICAL PHARMACOKINETICS, MECHANISM, DELIVERY, AGONIST, BREAST

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Citation

Please use this url to cite or link to this publication:

MLA
D’Hondt, Matthias, et al. “Hot-Melt Preparation of a Non-Biodegradable Peptide Implant : A Proof of Principle.” PROTEIN AND PEPTIDE LETTERS, vol. 26, no. 9, 2019, pp. 691–701.
APA
D’Hondt, M., Verbeke, F., Wuytens, P., Skirtach, A., De Spiegeleer, B., & Wynendaele, E. (2019). Hot-melt preparation of a non-biodegradable peptide implant : a proof of principle. PROTEIN AND PEPTIDE LETTERS, 26(9), 691–701.
Chicago author-date
D’Hondt, Matthias, Frederick Verbeke, Pieter Wuytens, Andre Skirtach, Bart De Spiegeleer, and Evelien Wynendaele. 2019. “Hot-Melt Preparation of a Non-Biodegradable Peptide Implant : A Proof of Principle.” PROTEIN AND PEPTIDE LETTERS 26 (9): 691–701.
Chicago author-date (all authors)
D’Hondt, Matthias, Frederick Verbeke, Pieter Wuytens, Andre Skirtach, Bart De Spiegeleer, and Evelien Wynendaele. 2019. “Hot-Melt Preparation of a Non-Biodegradable Peptide Implant : A Proof of Principle.” PROTEIN AND PEPTIDE LETTERS 26 (9): 691–701.
Vancouver
1.
D’Hondt M, Verbeke F, Wuytens P, Skirtach A, De Spiegeleer B, Wynendaele E. Hot-melt preparation of a non-biodegradable peptide implant : a proof of principle. PROTEIN AND PEPTIDE LETTERS. 2019;26(9):691–701.
IEEE
[1]
M. D’Hondt, F. Verbeke, P. Wuytens, A. Skirtach, B. De Spiegeleer, and E. Wynendaele, “Hot-melt preparation of a non-biodegradable peptide implant : a proof of principle,” PROTEIN AND PEPTIDE LETTERS, vol. 26, no. 9, pp. 691–701, 2019.
@article{8624981,
  abstract     = {Background: Both biodegradable and non-biodegradable peptide-loaded implants are already developed for the long-term treatment of patients, thereby reducing the frequency of drug administration. To further improve peptide formulation, extending the scope of implant-based drug delivery systems towards other polymers and processing techniques is highly interesting. 
Objective: In this study, as a proof-of-principle, the feasibility of hot-melt processing of a peptide active pharmaceutical ingredient was assessed by developing a non-biodegradable poly(ethylene- vinyl acetate) (33% VA) implant loaded with 20% (w/w) buserelin acetate. 
Methods: Cross-sectional implant characterization was performed by Raman microscopy. The stability of buserelin acetate in the polymeric matrix was evaluated for 3 months under ICH stability conditions and the quantity as well as the degradation products analyzed using LC-UV methods. An in vitro dissolution study was performed as well and buserelin acetate and its degradants analyzed using the same chromatographic methods. 
Results: No significant quantities of buserelin acetate-related degradation products were formed during the hot-melt preparation as well as during the stability study. Together with the consistent buserelin acetate assay values over time, chemical peptide stability was thus demonstrated. The in vitro buserelin acetate release from the implant was found to be diffusion-controlled after an initial burst release, with stable release profiles in the stability study, demonstrating the functional stability of the peptide implant. 
Conclusion: These results indicate the feasibility of preparing non-biodegradable peptide-loaded implants using the hot-melt production method and may act as a proof of principle concept for further innovation in peptide medicinal formulations.},
  author       = {D'Hondt, Matthias and Verbeke, Frederick and Wuytens, Pieter and Skirtach, Andre and De Spiegeleer, Bart and Wynendaele, Evelien},
  issn         = {0929-8665},
  journal      = {PROTEIN AND PEPTIDE LETTERS},
  keywords     = {Buserelin acetate,hot-melt process,poly(ethylene-vinyl acetate) polymeric formulation,analytical characterization,dissolution,stability,ETHYLENE-VINYL ACETATE,GONADOTROPIN-RELEASING-HORMONE,DRUG-RELEASE,SUSTAINED-RELEASE,IN-VITRO,CLINICAL PHARMACOKINETICS,MECHANISM,DELIVERY,AGONIST,BREAST},
  language     = {eng},
  number       = {9},
  pages        = {691--701},
  title        = {Hot-melt preparation of a non-biodegradable peptide implant : a proof of principle},
  url          = {http://dx.doi.org/10.2174/0929866526666190619113724},
  volume       = {26},
  year         = {2019},
}

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