Advanced search
1 file | 1.09 MB Add to list

A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. coli l-asparaginase

(2019) TALANTA. 203. p.9-15
Author
Organization
Abstract
Biosensors are rising technologies in the pharmaceutical field for medicine discovery, development and Quality Control (QC) stages. Surface acoustic wave (SAW) biosensor employs acoustic waves generated by oscillating a piezoelectric crystal quartz plate to meas. mass and viscosity, and allows to detect and quantify binding events between the analyte and an immobilized interacting ligand. We present here a SAW biosensor based approach for the functional quantification of Escherichia coli L-asparaginase (E. coli L-ASNase), using polyclonal antibody (pAb) as the interaction partner immobilized on the chip. Different immobilization strategies of pAb were initially evaluated, resulting in the BS3 activated amide coupling via protein G strategy as the final immobilization method. The method was validated by evaluating the selectivity, linearity, as well as accuracy (a recovery of 102.4%) and precision (RSD of 8.5%). The application of the validated method on different samples encompassing different lots of E. coli L-ASNase, deamidated E. coli L-ASNase and dry-heated E. coli L-ASNase (80 degrees C, 10 min) indicated the suitability of the developed SAW method to quantify E. colt L-ASNase. We suggest this SAW method can be adopted as a pharmaceutical QC method.
Keywords
Surface acoustic wave (SAW) biosensor, L-asparaginase, Method development and validation, HIGH-SENSITIVE DETECTION, LABEL-FREE, ANTIBODY IMMOBILIZATION, GOLD SURFACE, CANCER-CELLS, VALIDATION, THERAPY, ASSAYS, ARRAY

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.09 MB

Citation

Please use this url to cite or link to this publication:

MLA
Yao, Han, et al. “A Surface Acoustic Wave (SAW) Biosensor Method for Functional Quantification of E. Coli l-Asparaginase.” TALANTA, vol. 203, 2019, pp. 9–15.
APA
Yao, H., Soto Fernández, C., Xu, X., Wynendaele, E., & De Spiegeleer, B. (2019). A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. coli l-asparaginase. TALANTA, 203, 9–15.
Chicago author-date
Yao, Han, Cristina Soto Fernández, Xiaolong Xu, Evelien Wynendaele, and Bart De Spiegeleer. 2019. “A Surface Acoustic Wave (SAW) Biosensor Method for Functional Quantification of E. Coli l-Asparaginase.” TALANTA 203: 9–15.
Chicago author-date (all authors)
Yao, Han, Cristina Soto Fernández, Xiaolong Xu, Evelien Wynendaele, and Bart De Spiegeleer. 2019. “A Surface Acoustic Wave (SAW) Biosensor Method for Functional Quantification of E. Coli l-Asparaginase.” TALANTA 203: 9–15.
Vancouver
1.
Yao H, Soto Fernández C, Xu X, Wynendaele E, De Spiegeleer B. A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. coli l-asparaginase. TALANTA. 2019;203:9–15.
IEEE
[1]
H. Yao, C. Soto Fernández, X. Xu, E. Wynendaele, and B. De Spiegeleer, “A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. coli l-asparaginase,” TALANTA, vol. 203, pp. 9–15, 2019.
@article{8624977,
  abstract     = {{Biosensors are rising technologies in the pharmaceutical field for medicine discovery, development and Quality Control (QC) stages. Surface acoustic wave (SAW) biosensor employs acoustic waves generated by oscillating a piezoelectric crystal quartz plate to meas. mass and viscosity, and allows to detect and quantify binding events between the analyte and an immobilized interacting ligand. We present here a SAW biosensor based approach for the functional quantification of Escherichia coli L-asparaginase (E. coli L-ASNase), using polyclonal antibody (pAb) as the interaction partner immobilized on the chip. Different immobilization strategies of pAb were initially evaluated, resulting in the BS3 activated amide coupling via protein G strategy as the final immobilization method. The method was validated by evaluating the selectivity, linearity, as well as accuracy (a recovery of 102.4%) and precision (RSD of 8.5%). The application of the validated method on different samples encompassing different lots of E. coli L-ASNase, deamidated E. coli L-ASNase and dry-heated E. coli L-ASNase (80 degrees C, 10 min) indicated the suitability of the developed SAW method to quantify E. colt L-ASNase. We suggest this SAW method can be adopted as a pharmaceutical QC method.}},
  author       = {{Yao, Han and Soto Fernández, Cristina and Xu, Xiaolong and Wynendaele, Evelien and De Spiegeleer, Bart}},
  issn         = {{0039-9140}},
  journal      = {{TALANTA}},
  keywords     = {{Surface acoustic wave (SAW) biosensor,L-asparaginase,Method development and validation,HIGH-SENSITIVE DETECTION,LABEL-FREE,ANTIBODY IMMOBILIZATION,GOLD SURFACE,CANCER-CELLS,VALIDATION,THERAPY,ASSAYS,ARRAY}},
  language     = {{eng}},
  pages        = {{9--15}},
  title        = {{A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. coli l-asparaginase}},
  url          = {{http://dx.doi.org/10.1016/j.talanta.2019.05.046}},
  volume       = {{203}},
  year         = {{2019}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: