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A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. coli l-asparaginase

Han Yao, Cristina Soto Fernández, Xiaolong Xu, Evelien Wynendaele (UGent) and Bart De Spiegeleer (UGent)
(2019) TALANTA. 203. p.9-15
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Abstract
Biosensors are rising technologies in the pharmaceutical field for medicine discovery, development and Quality Control (QC) stages. Surface acoustic wave (SAW) biosensor employs acoustic waves generated by oscillating a piezoelectric crystal quartz plate to meas. mass and viscosity, and allows to detect and quantify binding events between the analyte and an immobilized interacting ligand. We present here a SAW biosensor based approach for the functional quantification of Escherichia coli L-asparaginase (E. coli L-ASNase), using polyclonal antibody (pAb) as the interaction partner immobilized on the chip. Different immobilization strategies of pAb were initially evaluated, resulting in the BS3 activated amide coupling via protein G strategy as the final immobilization method. The method was validated by evaluating the selectivity, linearity, as well as accuracy (a recovery of 102.4%) and precision (RSD of 8.5%). The application of the validated method on different samples encompassing different lots of E. coli L-ASNase, deamidated E. coli L-ASNase and dry-heated E. coli L-ASNase (80 degrees C, 10 min) indicated the suitability of the developed SAW method to quantify E. colt L-ASNase. We suggest this SAW method can be adopted as a pharmaceutical QC method.
Keywords
Surface acoustic wave (SAW) biosensor, L-asparaginase, Method development and validation, HIGH-SENSITIVE DETECTION, LABEL-FREE, ANTIBODY IMMOBILIZATION, GOLD SURFACE, CANCER-CELLS, VALIDATION, THERAPY, ASSAYS, ARRAY

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MLA
Yao, Han, et al. “A Surface Acoustic Wave (SAW) Biosensor Method for Functional Quantification of E. Coli l-Asparaginase.” TALANTA, vol. 203, 2019, pp. 9–15, doi:10.1016/j.talanta.2019.05.046.
APA
Yao, H., Soto Fernández, C., Xu, X., Wynendaele, E., & De Spiegeleer, B. (2019). A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. coli l-asparaginase. TALANTA, 203, 9–15. https://doi.org/10.1016/j.talanta.2019.05.046
Chicago author-date
Yao, Han, Cristina Soto Fernández, Xiaolong Xu, Evelien Wynendaele, and Bart De Spiegeleer. 2019. “A Surface Acoustic Wave (SAW) Biosensor Method for Functional Quantification of E. Coli l-Asparaginase.” TALANTA 203: 9–15. https://doi.org/10.1016/j.talanta.2019.05.046.
Chicago author-date (all authors)
Yao, Han, Cristina Soto Fernández, Xiaolong Xu, Evelien Wynendaele, and Bart De Spiegeleer. 2019. “A Surface Acoustic Wave (SAW) Biosensor Method for Functional Quantification of E. Coli l-Asparaginase.” TALANTA 203: 9–15. doi:10.1016/j.talanta.2019.05.046.
Vancouver
1.
Yao H, Soto Fernández C, Xu X, Wynendaele E, De Spiegeleer B. A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. coli l-asparaginase. TALANTA. 2019;203:9–15.
IEEE
[1]
H. Yao, C. Soto Fernández, X. Xu, E. Wynendaele, and B. De Spiegeleer, “A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. coli l-asparaginase,” TALANTA, vol. 203, pp. 9–15, 2019.
@article{8624977,
  abstract     = {{Biosensors are rising technologies in the pharmaceutical field for medicine discovery, development and Quality Control (QC) stages. Surface acoustic wave (SAW) biosensor employs acoustic waves generated by oscillating a piezoelectric crystal quartz plate to meas. mass and viscosity, and allows to detect and quantify binding events between the analyte and an immobilized interacting ligand. We present here a SAW biosensor based approach for the functional quantification of Escherichia coli L-asparaginase (E. coli L-ASNase), using polyclonal antibody (pAb) as the interaction partner immobilized on the chip. Different immobilization strategies of pAb were initially evaluated, resulting in the BS3 activated amide coupling via protein G strategy as the final immobilization method. The method was validated by evaluating the selectivity, linearity, as well as accuracy (a recovery of 102.4%) and precision (RSD of 8.5%). The application of the validated method on different samples encompassing different lots of E. coli L-ASNase, deamidated E. coli L-ASNase and dry-heated E. coli L-ASNase (80 degrees C, 10 min) indicated the suitability of the developed SAW method to quantify E. colt L-ASNase. We suggest this SAW method can be adopted as a pharmaceutical QC method.}},
  author       = {{Yao, Han and Soto Fernández, Cristina and Xu, Xiaolong and Wynendaele, Evelien and De Spiegeleer, Bart}},
  issn         = {{0039-9140}},
  journal      = {{TALANTA}},
  keywords     = {{Surface acoustic wave (SAW) biosensor,L-asparaginase,Method development and validation,HIGH-SENSITIVE DETECTION,LABEL-FREE,ANTIBODY IMMOBILIZATION,GOLD SURFACE,CANCER-CELLS,VALIDATION,THERAPY,ASSAYS,ARRAY}},
  language     = {{eng}},
  pages        = {{9--15}},
  title        = {{A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. coli l-asparaginase}},
  url          = {{http://doi.org/10.1016/j.talanta.2019.05.046}},
  volume       = {{203}},
  year         = {{2019}},
}
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