Bovine embryo-secreted microRNA-30c is a potential non-invasive biomarker for hampered preimplantation developmental competence
- Author
- Xiaoyuan Lin, Evy Beckers, Séan Mc Cafferty (UGent) , Yannick Gansemans (UGent) , Katarzyna Szymańska-Vandendriessche, Krishna Chaitanya Pavani (UGent) , João Portela Catani (UGent) , Filip Van Nieuwerburgh (UGent) , Dieter Deforce (UGent) , Petra De Sutter (UGent) , Ann Van Soom (UGent) and Luc Peelman (UGent)
- Organization
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- Department of Pharmaceutics
- Department of Basic and Applied Medical Sciences
- Department of Human Structure and Repair
- Ghent University Hospital
- Department of Nutrition, Genetics and Ethology (ceased 1-1-2022)
- Department of Obstetrics, reproduction and herd health (ceased 1-1-2022)
- Department of Internal Medicine, Reproduction and Population Medicine
- Abstract
- Recently, secreted microRNAs (miRNAs) have received a lot of attention since they may act as autocrine factors. However, how secreted miRNAs influence embryonic development is still poorly understood. We identified 294 miRNAs, 114 known, and 180 novel, in the conditioned medium of individually cultured bovine embryos. Of these miRNAs, miR-30c and miR-10b were much more abundant in conditioned medium of slow cleaving embryos compared to intermediate cleaving ones. MiR-10b, miR-novel44, and miR-novel-45 were higher expressed in the conditioned medium of degenerate embryos compared to blastocysts, while the reverse was observed for miR-novel113 and miR-novel-139. Supplementation of miR-30c mimics into the culture medium confirmed the uptake of miR-30c mimics by embryos and resulted in increased cell apoptosis, as also shown after delivery of miR-30c mimics in Madin-Darby bovine kidney cells (MDBKs). We also demonstrated that miR-30c directly targets Cyclindependent kinase 12 (CDK12) through its 3' untranslated region (3'-UTR) and inhibits its expression. Overexpression and downregulation of CDK12 revealed the opposite results of the delivery of miRNA-30c mimics and inhibitor. The significant down-regulation of several tested DNA damage response (DDR) genes, after increasing miR-30c or reducing CDK12 expression, suggests a possible role for miR-30c in regulating embryo development through DDR pathways.
- Keywords
- VITRO PRODUCED EMBRYOS, INNER CELL MASS, 1ST CLEAVAGE, EXPRESSION, ANALYSIS, CDK12, TROPHECTODERM, PREDICTS, CUMULUS, KINASE, TIME, bovine embryos, secreted miRNAs, miR-30c, CDK12, cell cycle, DNA damage, response, individual in vitro production
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8624386
- MLA
- Lin, Xiaoyuan, et al. “Bovine Embryo-Secreted MicroRNA-30c Is a Potential Non-Invasive Biomarker for Hampered Preimplantation Developmental Competence.” FRONTIERS IN GENETICS, vol. 10, 2019, doi:10.3389/fgene.2019.00315.
- APA
- Lin, X., Beckers, E., Mc Cafferty, S., Gansemans, Y., Szymańska-Vandendriessche, K., Pavani, K. C., … Peelman, L. (2019). Bovine embryo-secreted microRNA-30c is a potential non-invasive biomarker for hampered preimplantation developmental competence. FRONTIERS IN GENETICS, 10. https://doi.org/10.3389/fgene.2019.00315
- Chicago author-date
- Lin, Xiaoyuan, Evy Beckers, Séan Mc Cafferty, Yannick Gansemans, Katarzyna Szymańska-Vandendriessche, Krishna Chaitanya Pavani, João Portela Catani, et al. 2019. “Bovine Embryo-Secreted MicroRNA-30c Is a Potential Non-Invasive Biomarker for Hampered Preimplantation Developmental Competence.” FRONTIERS IN GENETICS 10. https://doi.org/10.3389/fgene.2019.00315.
- Chicago author-date (all authors)
- Lin, Xiaoyuan, Evy Beckers, Séan Mc Cafferty, Yannick Gansemans, Katarzyna Szymańska-Vandendriessche, Krishna Chaitanya Pavani, João Portela Catani, Filip Van Nieuwerburgh, Dieter Deforce, Petra De Sutter, Ann Van Soom, and Luc Peelman. 2019. “Bovine Embryo-Secreted MicroRNA-30c Is a Potential Non-Invasive Biomarker for Hampered Preimplantation Developmental Competence.” FRONTIERS IN GENETICS 10. doi:10.3389/fgene.2019.00315.
- Vancouver
- 1.Lin X, Beckers E, Mc Cafferty S, Gansemans Y, Szymańska-Vandendriessche K, Pavani KC, et al. Bovine embryo-secreted microRNA-30c is a potential non-invasive biomarker for hampered preimplantation developmental competence. FRONTIERS IN GENETICS. 2019;10.
- IEEE
- [1]X. Lin et al., “Bovine embryo-secreted microRNA-30c is a potential non-invasive biomarker for hampered preimplantation developmental competence,” FRONTIERS IN GENETICS, vol. 10, 2019.
@article{8624386,
abstract = {{Recently, secreted microRNAs (miRNAs) have received a lot of attention since they may act as autocrine factors. However, how secreted miRNAs influence embryonic development is still poorly understood. We identified 294 miRNAs, 114 known, and 180 novel, in the conditioned medium of individually cultured bovine embryos. Of these miRNAs, miR-30c and miR-10b were much more abundant in conditioned medium of slow cleaving embryos compared to intermediate cleaving ones. MiR-10b, miR-novel44, and miR-novel-45 were higher expressed in the conditioned medium of degenerate embryos compared to blastocysts, while the reverse was observed for miR-novel113 and miR-novel-139. Supplementation of miR-30c mimics into the culture medium confirmed the uptake of miR-30c mimics by embryos and resulted in increased cell apoptosis, as also shown after delivery of miR-30c mimics in Madin-Darby bovine kidney cells (MDBKs). We also demonstrated that miR-30c directly targets Cyclindependent kinase 12 (CDK12) through its 3' untranslated region (3'-UTR) and inhibits its expression. Overexpression and downregulation of CDK12 revealed the opposite results of the delivery of miRNA-30c mimics and inhibitor. The significant down-regulation of several tested DNA damage response (DDR) genes, after increasing miR-30c or reducing CDK12 expression, suggests a possible role for miR-30c in regulating embryo development through DDR pathways.}},
articleno = {{315}},
author = {{Lin, Xiaoyuan and Beckers, Evy and Mc Cafferty, Séan and Gansemans, Yannick and Szymańska-Vandendriessche, Katarzyna and Pavani, Krishna Chaitanya and Portela Catani, João and Van Nieuwerburgh, Filip and Deforce, Dieter and De Sutter, Petra and Van Soom, Ann and Peelman, Luc}},
issn = {{1664-8021}},
journal = {{FRONTIERS IN GENETICS}},
keywords = {{VITRO PRODUCED EMBRYOS,INNER CELL MASS,1ST CLEAVAGE,EXPRESSION,ANALYSIS,CDK12,TROPHECTODERM,PREDICTS,CUMULUS,KINASE,TIME,bovine embryos,secreted miRNAs,miR-30c,CDK12,cell cycle,DNA damage,response,individual in vitro production}},
language = {{eng}},
pages = {{15}},
title = {{Bovine embryo-secreted microRNA-30c is a potential non-invasive biomarker for hampered preimplantation developmental competence}},
url = {{http://doi.org/10.3389/fgene.2019.00315}},
volume = {{10}},
year = {{2019}},
}
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