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The generation and use of recombinant extracellular vesicles as biological reference material

Edward Geeurickx (UGent) , Joeri Tulkens (UGent) , Bert Dhondt (UGent) , Jan Van Deun (UGent) , Lien Lippens (UGent) , Glenn Vergauwen (UGent) , Elisa Heyrman, Delphine De Sutter (UGent) , Kris Gevaert (UGent) , Francis Impens (UGent) , et al.
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Abstract
Recent years have seen an increase of extracellular vesicle (EV) research geared towards biological understanding, diagnostics and therapy. However, EV data interpretation remains challenging owing to complexity of biofluids and technical variation introduced during sample preparation and analysis. To understand and mitigate these limitations, we generated trackable recombinant EV (rEV) as a biological reference material. Employing complementary characterization methods, we demonstrate that rEV are stable and bear physical and biochemical traits characteristic of sample EV. Furthermore, rEV can be quantified using fluorescence-, RNA- and protein-based technologies available in routine laboratories. Spiking rEV in biofluids allows recovery efficiencies of commonly implemented EV separation methods to be identified, intra-method and inter-user variability induced by sample handling to be defined, and to normalize and improve sensitivity of EV enumerations. We anticipate that rEV will aid EV-based sample preparation and analysis, data normalization, method development and instrument calibration in various research and biomedical applications.
Keywords
:EXOSOMES, DOMAINS, CELLS

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MLA
Geeurickx, Edward, et al. “The Generation and Use of Recombinant Extracellular Vesicles as Biological Reference Material.” NATURE COMMUNICATIONS, vol. 10, 2019.
APA
Geeurickx, E., Tulkens, J., Dhondt, B., Van Deun, J., Lippens, L., Vergauwen, G., … Hendrix, A. (2019). The generation and use of recombinant extracellular vesicles as biological reference material. NATURE COMMUNICATIONS, 10.
Chicago author-date
Geeurickx, Edward, Joeri Tulkens, Bert Dhondt, Jan Van Deun, Lien Lippens, Glenn Vergauwen, Elisa Heyrman, et al. 2019. “The Generation and Use of Recombinant Extracellular Vesicles as Biological Reference Material.” NATURE COMMUNICATIONS 10.
Chicago author-date (all authors)
Geeurickx, Edward, Joeri Tulkens, Bert Dhondt, Jan Van Deun, Lien Lippens, Glenn Vergauwen, Elisa Heyrman, Delphine De Sutter, Kris Gevaert, Francis Impens, Ilkka Miinalainen, Pieter-Jan Van Bockstal, Thomas De Beer, Marca HM Wauben, Esther NM Nolte-‘t-Hoen, Katarzyna Bloch, Johannes V Swinnen, Edwin van der Pol, Rienk Nieuwland, Geert Braems, Nico Callewaert, Pieter Mestdagh, Jo Vandesompele, Hannelore Denys, Sven Eyckerman, Olivier De Wever, and An Hendrix. 2019. “The Generation and Use of Recombinant Extracellular Vesicles as Biological Reference Material.” NATURE COMMUNICATIONS 10.
Vancouver
1.
Geeurickx E, Tulkens J, Dhondt B, Van Deun J, Lippens L, Vergauwen G, et al. The generation and use of recombinant extracellular vesicles as biological reference material. NATURE COMMUNICATIONS. 2019;10.
IEEE
[1]
E. Geeurickx et al., “The generation and use of recombinant extracellular vesicles as biological reference material,” NATURE COMMUNICATIONS, vol. 10, 2019.
@article{8623899,
  abstract     = {Recent years have seen an increase of extracellular vesicle (EV) research geared towards biological understanding, diagnostics and therapy. However, EV data interpretation remains challenging owing to complexity of biofluids and technical variation introduced during sample preparation and analysis. To understand and mitigate these limitations, we generated trackable recombinant EV (rEV) as a biological reference material. Employing complementary characterization methods, we demonstrate that rEV are stable and bear physical and biochemical traits characteristic of sample EV. Furthermore, rEV can be quantified using fluorescence-, RNA- and protein-based technologies available in routine laboratories. Spiking rEV in biofluids allows recovery efficiencies of commonly implemented EV separation methods to be identified, intra-method and inter-user variability induced by sample handling to be defined, and to normalize and improve sensitivity of EV enumerations. We anticipate that rEV will aid EV-based sample preparation and analysis, data normalization, method development and instrument calibration in various research and biomedical applications.},
  articleno    = {3288},
  author       = {Geeurickx, Edward and Tulkens, Joeri and Dhondt, Bert and Van Deun, Jan and Lippens, Lien and Vergauwen, Glenn and Heyrman, Elisa and De Sutter, Delphine and Gevaert, Kris and Impens, Francis and Miinalainen, Ilkka and Van Bockstal, Pieter-Jan and De Beer, Thomas and Wauben, Marca HM and Nolte-‘t-Hoen, Esther NM and Bloch, Katarzyna and Swinnen, Johannes V and van der Pol, Edwin and Nieuwland, Rienk and Braems, Geert and Callewaert, Nico and Mestdagh, Pieter and Vandesompele, Jo and Denys, Hannelore and Eyckerman, Sven and De Wever, Olivier and Hendrix, An},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keywords     = {:EXOSOMES,DOMAINS,CELLS},
  language     = {eng},
  pages        = {12},
  title        = {The generation and use of recombinant extracellular vesicles as biological reference material},
  url          = {http://dx.doi.org/10.1038/s41467-019-11182-0},
  volume       = {10},
  year         = {2019},
}

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