Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins
- Author
- Medea Padra, Barbara Adamczyk, Bram Flahou (UGent) , Mattias Erhardsson, Gurdeep Chahal, Annemieke Smet (UGent) , Chunsheng Jin, Anders Thorell, Richard Ducatelle (UGent) , Freddy Haesebrouck (UGent) , Niclas G Karlsson and Sara K Linden
- Organization
- Abstract
- Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma. H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers. Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche. Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals. Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs. Thus, Helicobacter spp. infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis. Inhibition of these mucus-based defenses creates a more stable and inhabitable niche for H. suis. This is likely of importance for long-term colonization and outcome of infection, and reversing these impairments may have therapeutic benefits.
- Keywords
- PYLORI STRAINS, DEPENDENT BINDING, HEILMANNII, EXPRESSION, COLONIZATION, MUCOSA, MUC5AC, IDENTIFICATION, PREVALENCE, DIVERSITY
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8623892
- MLA
- Padra, Medea, et al. “Helicobacter Suis Infection Alters Glycosylation and Decreases the Pathogen Growth Inhibiting Effect and Binding Avidity of Gastric Mucins.” MUCOSAL IMMUNOLOGY, vol. 12, no. 3, 2019, pp. 784–94, doi:10.1038/s41385-019-0154-4.
- APA
- Padra, M., Adamczyk, B., Flahou, B., Erhardsson, M., Chahal, G., Smet, A., … Linden, S. K. (2019). Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins. MUCOSAL IMMUNOLOGY, 12(3), 784–794. https://doi.org/10.1038/s41385-019-0154-4
- Chicago author-date
- Padra, Medea, Barbara Adamczyk, Bram Flahou, Mattias Erhardsson, Gurdeep Chahal, Annemieke Smet, Chunsheng Jin, et al. 2019. “Helicobacter Suis Infection Alters Glycosylation and Decreases the Pathogen Growth Inhibiting Effect and Binding Avidity of Gastric Mucins.” MUCOSAL IMMUNOLOGY 12 (3): 784–94. https://doi.org/10.1038/s41385-019-0154-4.
- Chicago author-date (all authors)
- Padra, Medea, Barbara Adamczyk, Bram Flahou, Mattias Erhardsson, Gurdeep Chahal, Annemieke Smet, Chunsheng Jin, Anders Thorell, Richard Ducatelle, Freddy Haesebrouck, Niclas G Karlsson, and Sara K Linden. 2019. “Helicobacter Suis Infection Alters Glycosylation and Decreases the Pathogen Growth Inhibiting Effect and Binding Avidity of Gastric Mucins.” MUCOSAL IMMUNOLOGY 12 (3): 784–794. doi:10.1038/s41385-019-0154-4.
- Vancouver
- 1.Padra M, Adamczyk B, Flahou B, Erhardsson M, Chahal G, Smet A, et al. Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins. MUCOSAL IMMUNOLOGY. 2019;12(3):784–94.
- IEEE
- [1]M. Padra et al., “Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins,” MUCOSAL IMMUNOLOGY, vol. 12, no. 3, pp. 784–794, 2019.
@article{8623892,
abstract = {{Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma. H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers. Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche. Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals. Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs. Thus, Helicobacter spp. infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis. Inhibition of these mucus-based defenses creates a more stable and inhabitable niche for H. suis. This is likely of importance for long-term colonization and outcome of infection, and reversing these impairments may have therapeutic benefits.}},
author = {{Padra, Medea and Adamczyk, Barbara and Flahou, Bram and Erhardsson, Mattias and Chahal, Gurdeep and Smet, Annemieke and Jin, Chunsheng and Thorell, Anders and Ducatelle, Richard and Haesebrouck, Freddy and Karlsson, Niclas G and Linden, Sara K}},
issn = {{1933-0219}},
journal = {{MUCOSAL IMMUNOLOGY}},
keywords = {{PYLORI STRAINS,DEPENDENT BINDING,HEILMANNII,EXPRESSION,COLONIZATION,MUCOSA,MUC5AC,IDENTIFICATION,PREVALENCE,DIVERSITY}},
language = {{eng}},
number = {{3}},
pages = {{784--794}},
title = {{Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins}},
url = {{http://doi.org/10.1038/s41385-019-0154-4}},
volume = {{12}},
year = {{2019}},
}
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