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Host intestinal biomarker identification in a gut leakage model in broilers

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Abstract
Intestinal health problems are a major issue in the poultry industry. Quantifiable easy-to-measure biomarkers for intestinal health would be of great value to monitor subclinical intestinal entities that cause performance problems and to evaluate control methods for intestinal health. The aim of the study was to identify host protein biomarkers for intestinal inflammation and intestinal barrier damage. Proteomic analysis was conducted on ileal and colonic content samples of broilers under an experimental gut damage and inflammation model. Effects of the challenge treatment resulted in a worse gut condition based on macroscopic gut appearance (p<0.0001). Also microscopic changes such as shortening of the villi and increased crypt depth (p<0.0001) as well as higher infiltration of T-lymphocytes (p<0.0001) were seen in the duodenal tissue of challenged animals. Several candidate proteins associated with inflammation, serum leakage and/or tissue damage were identified with an increased abundance in intestinal content of challenged animals (p<0.05). Conversely, brush border enzymes were less abundant in intestinal content of challenged animals (p<0.05). These candidate biomarkers have potential to be used in the field for detection of gut barrier failure in broilers.
Keywords
FECAL CALPROTECTIN, PERMEABILITY, LOCALIZATION, INFLAMMATION, FIBRONECTIN, PERFORMANCE, EXPRESSION, INTEGRITY, BATTERY, HEALTH

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Chicago
De Meyer, Fien, Venessa Eeckhaut, Richard Ducatelle, Maarten Dhaenens, Simon Daled, Annelike Dedeurwaerder, Maarten De Gussem, Freddy Haesebrouck, Dieter Deforce, and Filip Van Immerseel. 2019. “Host Intestinal Biomarker Identification in a Gut Leakage Model in Broilers.” Veterinary Research 50.
APA
De Meyer, Fien, Eeckhaut, V., Ducatelle, R., Dhaenens, M., Daled, S., Dedeurwaerder, A., De Gussem, M., et al. (2019). Host intestinal biomarker identification in a gut leakage model in broilers. VETERINARY RESEARCH, 50.
Vancouver
1.
De Meyer F, Eeckhaut V, Ducatelle R, Dhaenens M, Daled S, Dedeurwaerder A, et al. Host intestinal biomarker identification in a gut leakage model in broilers. VETERINARY RESEARCH. London: Bmc; 2019;50.
MLA
De Meyer, Fien et al. “Host Intestinal Biomarker Identification in a Gut Leakage Model in Broilers.” VETERINARY RESEARCH 50 (2019): n. pag. Print.
@article{8623888,
  abstract     = {Intestinal health problems are a major issue in the poultry industry. Quantifiable easy-to-measure biomarkers for intestinal health would be of great value to monitor subclinical intestinal entities that cause performance problems and to evaluate control methods for intestinal health. The aim of the study was to identify host protein biomarkers for intestinal inflammation and intestinal barrier damage. Proteomic analysis was conducted on ileal and colonic content samples of broilers under an experimental gut damage and inflammation model. Effects of the challenge treatment resulted in a worse gut condition based on macroscopic gut appearance (p<0.0001). Also microscopic changes such as shortening of the villi and increased crypt depth (p<0.0001) as well as higher infiltration of T-lymphocytes (p<0.0001) were seen in the duodenal tissue of challenged animals. Several candidate proteins associated with inflammation, serum leakage and/or tissue damage were identified with an increased abundance in intestinal content of challenged animals (p<0.05). Conversely, brush border enzymes were less abundant in intestinal content of challenged animals (p<0.05). These candidate biomarkers have potential to be used in the field for detection of gut barrier failure in broilers.},
  articleno    = {46},
  author       = {De Meyer, Fien and Eeckhaut, Venessa and Ducatelle, Richard and Dhaenens, Maarten and Daled, Simon and Dedeurwaerder, Annelike and De Gussem, Maarten and Haesebrouck, Freddy and Deforce, Dieter and Van Immerseel, Filip},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keywords     = {FECAL CALPROTECTIN,PERMEABILITY,LOCALIZATION,INFLAMMATION,FIBRONECTIN,PERFORMANCE,EXPRESSION,INTEGRITY,BATTERY,HEALTH},
  language     = {eng},
  pages        = {14},
  publisher    = {Bmc},
  title        = {Host intestinal biomarker identification in a gut leakage model in broilers},
  url          = {http://dx.doi.org/10.1186/s13567-019-0663-x},
  volume       = {50},
  year         = {2019},
}

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