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Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates

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Abstract
The impact of cortisol on Flavobacterium columnare biofilm formation was explored. Firstly, the dynamics of biofilm formation by one highly (HV) and one low virulent (LV) F. columnare isolate with and without the stress hormone cortisol under microfluidic flow conditions was characterized. This to confirm that F. columnare cells could form biofilm under cortisol supplementation, and to compare the temporal and structural differences between different treatment groups. One trial revealed that in both isolates cell aggregates resembling biofilms occurred within 7-h post-inoculation. Consequently, cell clusters were sloughed away, followed by a rebuilding of bacterial cell aggregates, suggestive for a high spreading capacity. While the HV isolate revealed cell aggregates formed upstream at all time-points, for the LV isolate this was only seen upon cortisol supplementation. Secondly, the transcriptional effect of genes (gldK, gldL, gldM, gldN, sprA, sprE, sprT, and porV) belonging to the Type IX secretion system involved in gliding motility was investigated in planktonic and biofilm cells of a HV and LV isolate to which no, a low (LD) or high (HD) dose of cortisol was added. Significantly lower expression of gliding genes gldK, gldL, gldM and gldN, and of protein secretion regulator porV was seen in the LV isolate planktonic cells supplemented with a HD-cortisol. The LV isolate biofilm cells treated with the HD-cortisol showed a significant upregulation of sprT, encoding mobile surface adhesion important in bacterial colonization. This is the first evidence for the co-regulatory effect of cortisol on biofilm formation and F. columnare gliding gene expression.
Keywords
GLIDING MOTILITY, FISH PATHOGEN, FLEXIBACTER-COLUMNARIS, SECRETION, ADHESION, EXPRESSION, DIVERSITY, VIRULENCE, DISEASE, GENES

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Chicago
Declercq, Annelies Maria, Wenlong Cai, Eber Naranjo, Wilawan Thongda, Venessa Eeckhaut, Eva Bauwens, Covadonga Arias, et al. 2019. “Evidence That the Stress Hormone Cortisol Regulates Biofilm Formation Differently Among Flavobacterium Columnare Isolates.” Veterinary Research 50.
APA
Declercq, A. M., Cai, W., Naranjo, E., Thongda, W., Eeckhaut, V., Bauwens, E., Arias, C., et al. (2019). Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates. VETERINARY RESEARCH, 50.
Vancouver
1.
Declercq AM, Cai W, Naranjo E, Thongda W, Eeckhaut V, Bauwens E, et al. Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates. VETERINARY RESEARCH. London: Bmc; 2019;50.
MLA
Declercq, Annelies Maria et al. “Evidence That the Stress Hormone Cortisol Regulates Biofilm Formation Differently Among Flavobacterium Columnare Isolates.” VETERINARY RESEARCH 50 (2019): n. pag. Print.
@article{8623882,
  abstract     = {The impact of cortisol on Flavobacterium columnare biofilm formation was explored. Firstly, the dynamics of biofilm formation by one highly (HV) and one low virulent (LV) F. columnare isolate with and without the stress hormone cortisol under microfluidic flow conditions was characterized. This to confirm that F. columnare cells could form biofilm under cortisol supplementation, and to compare the temporal and structural differences between different treatment groups. One trial revealed that in both isolates cell aggregates resembling biofilms occurred within 7-h post-inoculation. Consequently, cell clusters were sloughed away, followed by a rebuilding of bacterial cell aggregates, suggestive for a high spreading capacity. While the HV isolate revealed cell aggregates formed upstream at all time-points, for the LV isolate this was only seen upon cortisol supplementation. Secondly, the transcriptional effect of genes (gldK, gldL, gldM, gldN, sprA, sprE, sprT, and porV) belonging to the Type IX secretion system involved in gliding motility was investigated in planktonic and biofilm cells of a HV and LV isolate to which no, a low (LD) or high (HD) dose of cortisol was added. Significantly lower expression of gliding genes gldK, gldL, gldM and gldN, and of protein secretion regulator porV was seen in the LV isolate planktonic cells supplemented with a HD-cortisol. The LV isolate biofilm cells treated with the HD-cortisol showed a significant upregulation of sprT, encoding mobile surface adhesion important in bacterial colonization. This is the first evidence for the co-regulatory effect of cortisol on biofilm formation and F. columnare gliding gene expression.},
  articleno    = {24},
  author       = {Declercq, Annelies Maria and Cai, Wenlong and Naranjo, Eber and Thongda, Wilawan and Eeckhaut, Venessa and Bauwens, Eva and Arias, Covadonga and De La Fuente, Leonardo and Beck, Benjamin H. and Lange, Miles D. and Peatman, Eric and Haesebrouck, Freddy and Aerts, Johan and Decostere, Annemie},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keywords     = {GLIDING MOTILITY,FISH PATHOGEN,FLEXIBACTER-COLUMNARIS,SECRETION,ADHESION,EXPRESSION,DIVERSITY,VIRULENCE,DISEASE,GENES},
  language     = {eng},
  pages        = {11},
  publisher    = {Bmc},
  title        = {Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates},
  url          = {http://dx.doi.org/10.1186/s13567-019-0641-3},
  volume       = {50},
  year         = {2019},
}

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