Advanced search
1 file | 1.22 MB

Scrutinizing the triad of Vibrio tapetis, the skin barrier and pigmentation as determining factors in the development of skin ulcerations in wild common dab (Limanda limanda)

Author
Organization
Abstract
Recently, Vibrio tapetis was isolated for the first time from skin ulcerations in wild-caught common dab (Limanda limanda). To further examine its role in the development of these skin lesions, an in vivo experiment was performed. The significance of the skin barrier and in addition the difference between pigmented and non-pigmented side were investigated. Hence, the skin of common dab was treated in three different ways on both the pigmented and non-pigmented side. On a first treatment zone, the scales and overlying epidermal tissue were removed whereas in a second zone only the mucus was discarded. The third zone served as a non-treated zone. Thereafter, fish were challenged with V. tapetis. The control group was sham treated. Mortality, clinical signs, severity and size of the developing lesions were recorded. All animals were sacrificed and sampled 21days post-inoculation. Significantly more fish of the group challenged with V. tapetis died compared to the control group with the highest incidence occurring 4days post-inoculation. Fish challenged with V. tapetis developed more severe skin ulcerations. In zones where scales and epidermal tissue were removed, the ulcerations were more severe compared to zones where only mucus was eliminated. Ulcerations occurred more frequently, were more severe and larger on the pigmented side. Our data represents prove of V. tapetis as causative agent of ulcerative skin lesions although prior damage of the skin seems to be a major contributing factor. Furthermore, the pigmented side seemed predisposed to the development of skin ulcerations.
Keywords
FLOUNDER PLATICHTHYS-FLESUS, DUTCH WADDEN SEA, ATLANTIC SALMON, EPIDERMAL PAPILLOMA, SYMPHODUS-MELOPS, CORKWING WRASSE, ULCER DISEASE, STRAINS, STRESS, MUCUS

Downloads

  • vibrio.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.22 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Vercauteren, Maaike, Evelien De Swaef, Annelies M. Declercq, Hans Polet, Johan Aerts, Bart Ampe, Jesus L. Romalde, et al. 2019. “Scrutinizing the Triad of Vibrio Tapetis, the Skin Barrier and Pigmentation as Determining Factors in the Development of Skin Ulcerations in Wild Common Dab (Limanda Limanda).” Veterinary Research 50.
APA
Vercauteren, Maaike, De Swaef, E., Declercq, A. M., Polet, H., Aerts, J., Ampe, B., Romalde, J. L., et al. (2019). Scrutinizing the triad of Vibrio tapetis, the skin barrier and pigmentation as determining factors in the development of skin ulcerations in wild common dab (Limanda limanda). VETERINARY RESEARCH, 50.
Vancouver
1.
Vercauteren M, De Swaef E, Declercq AM, Polet H, Aerts J, Ampe B, et al. Scrutinizing the triad of Vibrio tapetis, the skin barrier and pigmentation as determining factors in the development of skin ulcerations in wild common dab (Limanda limanda). VETERINARY RESEARCH. London: Bmc; 2019;50.
MLA
Vercauteren, Maaike et al. “Scrutinizing the Triad of Vibrio Tapetis, the Skin Barrier and Pigmentation as Determining Factors in the Development of Skin Ulcerations in Wild Common Dab (Limanda Limanda).” VETERINARY RESEARCH 50 (2019): n. pag. Print.
@article{8623880,
  abstract     = {Recently, Vibrio tapetis was isolated for the first time from skin ulcerations in wild-caught common dab (Limanda limanda). To further examine its role in the development of these skin lesions, an in vivo experiment was performed. The significance of the skin barrier and in addition the difference between pigmented and non-pigmented side were investigated. Hence, the skin of common dab was treated in three different ways on both the pigmented and non-pigmented side. On a first treatment zone, the scales and overlying epidermal tissue were removed whereas in a second zone only the mucus was discarded. The third zone served as a non-treated zone. Thereafter, fish were challenged with V. tapetis. The control group was sham treated. Mortality, clinical signs, severity and size of the developing lesions were recorded. All animals were sacrificed and sampled 21days post-inoculation. Significantly more fish of the group challenged with V. tapetis died compared to the control group with the highest incidence occurring 4days post-inoculation. Fish challenged with V. tapetis developed more severe skin ulcerations. In zones where scales and epidermal tissue were removed, the ulcerations were more severe compared to zones where only mucus was eliminated. Ulcerations occurred more frequently, were more severe and larger on the pigmented side. Our data represents prove of V. tapetis as causative agent of ulcerative skin lesions although prior damage of the skin seems to be a major contributing factor. Furthermore, the pigmented side seemed predisposed to the development of skin ulcerations.},
  articleno    = {41},
  author       = {Vercauteren, Maaike and De Swaef, Evelien and Declercq, Annelies M. and Polet, Hans and Aerts, Johan and Ampe, Bart and Romalde, Jesus L. and Haesebrouck, Freddy and Devriese, Lisa and Decostere, Annemie and Chiers, Koen},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keywords     = {FLOUNDER PLATICHTHYS-FLESUS,DUTCH WADDEN SEA,ATLANTIC SALMON,EPIDERMAL PAPILLOMA,SYMPHODUS-MELOPS,CORKWING WRASSE,ULCER DISEASE,STRAINS,STRESS,MUCUS},
  language     = {eng},
  pages        = {13},
  publisher    = {Bmc},
  title        = {Scrutinizing the triad of Vibrio tapetis, the skin barrier and pigmentation as determining factors in the development of skin ulcerations in wild common dab (Limanda limanda)},
  url          = {http://dx.doi.org/10.1186/s13567-019-0659-6},
  volume       = {50},
  year         = {2019},
}

Altmetric
View in Altmetric
Web of Science
Times cited: