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The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion

Laurent Thierens (UGent) , SOPHIE MOERMAN (UGent) , Charlotte Van Elst (UGent) , Chris Vercruysse (UGent) , Petra Maes, Liesbeth Temmerman (UGent) , Noëmi De Roo (UGent) , Ronald Verbeeck (UGent) and Guy De Pauw (UGent)
Author
Organization
Abstract
Objective: The aim of this in vitro study was to determine the effects of remineralization promoting agents containing casein phosphopeptide-stabilized amorphous calcium phosphate (CPP-ACP), or CPP-ACP in combination with fluoride (CPP-ACPF) on artificial white spot lesions (WSLs) after 6 and 12 weeks. Methodology: White spot lesions were created on 123 sectioned premolars (246 specimens) with a demineralization solution during a 96 hours pH-cycling regime. Two experimental groups were created: a CPP-ACP group (Tooth Mousse (TM)), and a CPP-ACPF group (Mi Paste Plus (TM)). Additionally, two control groups were created, one using only a conventional toothpaste (1450 ppm fluoride) and another one without any working agents. All teeth were also daily brushed with the conventional toothpaste except the second control group. Tooth Mousse (TM) and Mi Paste Plus (TM) were applied for 180 seconds every day. The volume of demineralization was measured with transverse microradiography. Six lesion characteristics regarding the lesion depth and mineral content of WSLs were also determined. Results: The application of CPP-ACP and CPP-ACPF had a significant regenerative effect on the WSLs. Compared to Control group 1 and 2 the volume of demineralization after 6 weeks decreased significantly for CPP-ACP (respectively p<0.001 and p<0.001) and CPP-ACPF (respectively p=0.001 and p=0.003). The same trend was observed after 12 weeks. For the CPP-ACPF group, WSL dimensions decreased significantly between 6 and 12 weeks follow-up (p=0.012). The lesion depth reduced significantly after application of CPP-ACP and CPP-ACPF but increased significantly in the Control groups. Mineral content increased for CPP-ACP and CPP-ACPF after an application period of 12 weeks, but this was only significant for CPP-ACP. Conclusions: Long-term use of CPP-ACP and CPP-ACPF in combination with a conventional tooth paste shows beneficial effects in the recovery of in vitro subsurface caries lesions.
Keywords
Tooth remineralization, Dental white spots, In vitro, AMORPHOUS CALCIUM-PHOSPHATE, WHITE-SPOT LESIONS, ENAMEL SUBSURFACE LESIONS, MI PASTE PLUS, ORTHODONTIC TREATMENT, FLUORIDE, DEMINERALIZATION, VARNISH

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MLA
Thierens, Laurent, et al. “The in Vitro Remineralizing Effect of CPP-ACP and CPP-ACPF after 6 and 12 Weeks on Initial Caries Lesion.” JOURNAL OF APPLIED ORAL SCIENCE, vol. 27, 2019, doi:10.1590/1678-7757-2018-0589.
APA
Thierens, L., MOERMAN, S., Van Elst, C., Vercruysse, C., Maes, P., Temmerman, L., … De Pauw, G. (2019). The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion. JOURNAL OF APPLIED ORAL SCIENCE, 27. https://doi.org/10.1590/1678-7757-2018-0589
Chicago author-date
Thierens, Laurent, SOPHIE MOERMAN, Charlotte Van Elst, Chris Vercruysse, Petra Maes, Liesbeth Temmerman, Noëmi De Roo, Ronald Verbeeck, and Guy De Pauw. 2019. “The in Vitro Remineralizing Effect of CPP-ACP and CPP-ACPF after 6 and 12 Weeks on Initial Caries Lesion.” JOURNAL OF APPLIED ORAL SCIENCE 27. https://doi.org/10.1590/1678-7757-2018-0589.
Chicago author-date (all authors)
Thierens, Laurent, SOPHIE MOERMAN, Charlotte Van Elst, Chris Vercruysse, Petra Maes, Liesbeth Temmerman, Noëmi De Roo, Ronald Verbeeck, and Guy De Pauw. 2019. “The in Vitro Remineralizing Effect of CPP-ACP and CPP-ACPF after 6 and 12 Weeks on Initial Caries Lesion.” JOURNAL OF APPLIED ORAL SCIENCE 27. doi:10.1590/1678-7757-2018-0589.
Vancouver
1.
Thierens L, MOERMAN S, Van Elst C, Vercruysse C, Maes P, Temmerman L, et al. The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion. JOURNAL OF APPLIED ORAL SCIENCE. 2019;27.
IEEE
[1]
L. Thierens et al., “The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion,” JOURNAL OF APPLIED ORAL SCIENCE, vol. 27, 2019.
@article{8623126,
  abstract     = {{Objective: The aim of this in vitro study was to determine the effects of remineralization promoting agents containing casein phosphopeptide-stabilized amorphous calcium phosphate (CPP-ACP), or CPP-ACP in combination with fluoride (CPP-ACPF) on artificial white spot lesions (WSLs) after 6 and 12 weeks.
Methodology: White spot lesions were created on 123 sectioned premolars (246 specimens) with a demineralization solution during a 96 hours pH-cycling regime. Two experimental groups were created: a CPP-ACP group (Tooth Mousse (TM)), and a CPP-ACPF group (Mi Paste Plus (TM)). Additionally, two control groups were created, one using only a conventional toothpaste (1450 ppm fluoride) and another one without any working agents. All teeth were also daily brushed with the conventional toothpaste except the second control group. Tooth Mousse (TM) and Mi Paste Plus (TM) were applied for 180 seconds every day. The volume of demineralization was measured with transverse microradiography. Six lesion characteristics regarding the lesion depth and mineral content of WSLs were also determined.
Results: The application of CPP-ACP and CPP-ACPF had a significant regenerative effect on the WSLs. Compared to Control group 1 and 2 the volume of demineralization after 6 weeks decreased significantly for CPP-ACP (respectively p<0.001 and p<0.001) and CPP-ACPF (respectively p=0.001 and p=0.003). The same trend was observed after 12 weeks. For the CPP-ACPF group, WSL dimensions decreased significantly between 6 and 12 weeks follow-up (p=0.012). The lesion depth reduced significantly after application of CPP-ACP and CPP-ACPF but increased significantly in the Control groups. Mineral content increased for CPP-ACP and CPP-ACPF after an application period of 12 weeks, but this was only significant for CPP-ACP.
Conclusions: Long-term use of CPP-ACP and CPP-ACPF in combination with a conventional tooth paste shows beneficial effects in the recovery of in vitro subsurface caries lesions.}},
  articleno    = {{e20180589}},
  author       = {{Thierens, Laurent and MOERMAN, SOPHIE and Van Elst, Charlotte and Vercruysse, Chris and Maes, Petra and Temmerman, Liesbeth and De Roo, Noëmi and Verbeeck, Ronald and De Pauw, Guy}},
  issn         = {{1678-7757}},
  journal      = {{JOURNAL OF APPLIED ORAL SCIENCE}},
  keywords     = {{Tooth remineralization,Dental white spots,In vitro,AMORPHOUS CALCIUM-PHOSPHATE,WHITE-SPOT LESIONS,ENAMEL SUBSURFACE LESIONS,MI PASTE PLUS,ORTHODONTIC TREATMENT,FLUORIDE,DEMINERALIZATION,VARNISH}},
  language     = {{eng}},
  pages        = {{9}},
  title        = {{The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion}},
  url          = {{http://dx.doi.org/10.1590/1678-7757-2018-0589}},
  volume       = {{27}},
  year         = {{2019}},
}

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