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25-Hydroxycholesterol provides antiviral protection against highly pathogenic porcine reproductive and respiratory syndrome virus in swine

(2019) VETERINARY MICROBIOLOGY. 231. p.63-70
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Abstract
Porcine reproductive and respiratory syndrome (PRRS) is a severe respiratory disease that leads to huge economic losses in the pig industry throughout the world. Although there are several vaccines available, the protective efficacy is limited. Therefore, new control strategies to prevent PRRS virus (PRRSV) infection are urgently required. We have previously reported that CH25H and 25HC can significantly inhibit the replication of PRRSV by preventing viral entry. In the present study, we found that 25HC with a low IC50 value significantly decreased the replication of different PRRSV strains, and increased the production of IL-1 beta and IL-8 in porcine primary alveolar macrophages and the lung tissue. In pigs challenged with highly pathogenic PRRSV, treatment with 25HC was associated with an obvious reduction in the level of viremia and viral load in lung samples and nasal swabs, as well as decreased lung injury and an increased survival rate. These findings suggest that 25HC could be a promising antiviral drug against PRRSV in the future.
Keywords
25-Hydroxycholesterol, PRRSV, Antiviral activity, HOST RESTRICTION FACTOR, CHOLESTEROL 25-HYDROXYLASE, IDENTIFICATION, REPLICATION, STRAIN, ACTS

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MLA
Song, Zhongbao et al. “25-Hydroxycholesterol Provides Antiviral Protection Against Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in Swine.” VETERINARY MICROBIOLOGY 231 (2019): 63–70. Print.
APA
Song, Zhongbao, Bai, J., Nauwynck, H., Lin, L., Liu, X., Yu, J., & Jiang, P. (2019). 25-Hydroxycholesterol provides antiviral protection against highly pathogenic porcine reproductive and respiratory syndrome virus in swine. VETERINARY MICROBIOLOGY, 231, 63–70.
Chicago author-date
Song, Zhongbao, Juan Bai, Hans Nauwynck, Lv Lin, Xuewei Liu, Jie Yu, and Ping Jiang. 2019. “25-Hydroxycholesterol Provides Antiviral Protection Against Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in Swine.” Veterinary Microbiology 231: 63–70.
Chicago author-date (all authors)
Song, Zhongbao, Juan Bai, Hans Nauwynck, Lv Lin, Xuewei Liu, Jie Yu, and Ping Jiang. 2019. “25-Hydroxycholesterol Provides Antiviral Protection Against Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in Swine.” Veterinary Microbiology 231: 63–70.
Vancouver
1.
Song Z, Bai J, Nauwynck H, Lin L, Liu X, Yu J, et al. 25-Hydroxycholesterol provides antiviral protection against highly pathogenic porcine reproductive and respiratory syndrome virus in swine. VETERINARY MICROBIOLOGY. 2019;231:63–70.
IEEE
[1]
Z. Song et al., “25-Hydroxycholesterol provides antiviral protection against highly pathogenic porcine reproductive and respiratory syndrome virus in swine,” VETERINARY MICROBIOLOGY, vol. 231, pp. 63–70, 2019.
@article{8621526,
  abstract     = {Porcine reproductive and respiratory syndrome (PRRS) is a severe respiratory disease that leads to huge economic losses in the pig industry throughout the world. Although there are several vaccines available, the protective efficacy is limited. Therefore, new control strategies to prevent PRRS virus (PRRSV) infection are urgently required. We have previously reported that CH25H and 25HC can significantly inhibit the replication of PRRSV by preventing viral entry. In the present study, we found that 25HC with a low IC50 value significantly decreased the replication of different PRRSV strains, and increased the production of IL-1 beta and IL-8 in porcine primary alveolar macrophages and the lung tissue. In pigs challenged with highly pathogenic PRRSV, treatment with 25HC was associated with an obvious reduction in the level of viremia and viral load in lung samples and nasal swabs, as well as decreased lung injury and an increased survival rate. These findings suggest that 25HC could be a promising antiviral drug against PRRSV in the future.},
  author       = {Song, Zhongbao and Bai, Juan and Nauwynck, Hans and Lin, Lv and Liu, Xuewei and Yu, Jie and Jiang, Ping},
  issn         = {0378-1135},
  journal      = {VETERINARY MICROBIOLOGY},
  keywords     = {25-Hydroxycholesterol,PRRSV,Antiviral activity,HOST RESTRICTION FACTOR,CHOLESTEROL 25-HYDROXYLASE,IDENTIFICATION,REPLICATION,STRAIN,ACTS},
  language     = {eng},
  pages        = {63--70},
  title        = {25-Hydroxycholesterol provides antiviral protection against highly pathogenic porcine reproductive and respiratory syndrome virus in swine},
  url          = {http://dx.doi.org/10.1016/j.vetmic.2019.02.035},
  volume       = {231},
  year         = {2019},
}

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