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Reducing Campylobacter jejuni colonization in broiler chickens by in-feed supplementation with hyperimmune egg yolk antibodies

Jasmien Vandeputte (UGent) , An Martel (UGent) , Stefano Canessa (UGent) , Nathalie Van Rysselberghe (UGent) , Lieven De Zutter (UGent) , Marc Heyndrickx (UGent) , Freddy Haesebrouck (UGent) , Frank Pasmans (UGent) and An Garmyn (UGent)
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Abstract
Campylobacter infections sourced mainly to poultry products, are the most important bacterial foodborne zoonoses worldwide. No effective measures to control these infections in broiler production exist to date. Here, we used passive immunization with hyperimmune egg yolks to confer broad protection of broilers against Campylobacter infection. Two novel vaccines, a bacterin of thirteen Campylobacter jejuni (C. jejuni) and C. coli strains and a subunit vaccine of six immunodominant Campylobacter antigens, were used for the immunization of layers, resulting in high and prolonged levels of specific immunoglobulinY (IgY) in the hens' yolks. In the first in vivo trial, yolks (sham, bacterin or subunit vaccine derived) were administered prophylactically in the broiler feed. Both the bacterin and subunit vaccine-induced IgY significantly reduced the number of Campylobacter-colonized broilers. In the second in vivo trial, the yolks were administered therapeutically during three days before euthanasia. The bacterin IgY resulted in a significant decrease in C. jejuni counts per infected bird. The hyperimmune yolks showed strong reactivity to a broad representation of C. jejuni and C. coli clonal complexes. These results indicate that passive immunization with hyperimmune yolks, especially bacterin derived, offers possibilities to control Campylobacter colonization in poultry.
Keywords
MATERNAL ANTIBODIES, CHAPERONIN GROEL, PROTEINS, CONTAMINATION, INFECTION, POULTRY, IGY

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Chicago
Vandeputte, Jasmien, An Martel, Stefano Canessa, Nathalie Van Rysselberghe, Lieven De Zutter, Marc Heyndrickx, Freddy Haesebrouck, Frank Pasmans, and An Garmyn. 2019. “Reducing Campylobacter Jejuni Colonization in Broiler Chickens by In-feed Supplementation with Hyperimmune Egg Yolk Antibodies.” Scientific Reports 9.
APA
Vandeputte, J., Martel, A., Canessa, S., Van Rysselberghe, N., De Zutter, L., Heyndrickx, M., Haesebrouck, F., et al. (2019). Reducing Campylobacter jejuni colonization in broiler chickens by in-feed supplementation with hyperimmune egg yolk antibodies. SCIENTIFIC REPORTS, 9.
Vancouver
1.
Vandeputte J, Martel A, Canessa S, Van Rysselberghe N, De Zutter L, Heyndrickx M, et al. Reducing Campylobacter jejuni colonization in broiler chickens by in-feed supplementation with hyperimmune egg yolk antibodies. SCIENTIFIC REPORTS. 2019;9.
MLA
Vandeputte, Jasmien et al. “Reducing Campylobacter Jejuni Colonization in Broiler Chickens by In-feed Supplementation with Hyperimmune Egg Yolk Antibodies.” SCIENTIFIC REPORTS 9 (2019): n. pag. Print.
@article{8621158,
  abstract     = {Campylobacter infections sourced mainly to poultry products, are the most important bacterial foodborne zoonoses worldwide. No effective measures to control these infections in broiler production exist to date. Here, we used passive immunization with hyperimmune egg yolks to confer broad protection of broilers against Campylobacter infection. Two novel vaccines, a bacterin of thirteen Campylobacter jejuni (C. jejuni) and C. coli strains and a subunit vaccine of six immunodominant Campylobacter antigens, were used for the immunization of layers, resulting in high and prolonged levels of specific immunoglobulinY (IgY) in the hens' yolks. In the first in vivo trial, yolks (sham, bacterin or subunit vaccine derived) were administered prophylactically in the broiler feed. Both the bacterin and subunit vaccine-induced IgY significantly reduced the number of Campylobacter-colonized broilers. In the second in vivo trial, the yolks were administered therapeutically during three days before euthanasia. The bacterin IgY resulted in a significant decrease in C. jejuni counts per infected bird. The hyperimmune yolks showed strong reactivity to a broad representation of C. jejuni and C. coli clonal complexes. These results indicate that passive immunization with hyperimmune yolks, especially bacterin derived, offers possibilities to control Campylobacter colonization in poultry.},
  articleno    = {8931},
  author       = {Vandeputte, Jasmien and Martel, An and Canessa, Stefano and Van Rysselberghe, Nathalie and De Zutter, Lieven and Heyndrickx, Marc and Haesebrouck, Freddy and Pasmans, Frank and Garmyn, An},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {MATERNAL ANTIBODIES,CHAPERONIN GROEL,PROTEINS,CONTAMINATION,INFECTION,POULTRY,IGY},
  language     = {eng},
  pages        = {10},
  title        = {Reducing Campylobacter jejuni colonization in broiler chickens by in-feed supplementation with hyperimmune egg yolk antibodies},
  url          = {http://dx.doi.org/10.1038/s41598-019-45380-z},
  volume       = {9},
  year         = {2019},
}

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