Advanced search
1 file | 204.37 KB Add to list

Optimizing identification of Mycoplasma bovis by MALDI-TOF MS

Jade Bokma (UGent) , Bart Pardon (UGent) , Laura Van Driessche (UGent) , Linde Gille (UGent) , Piet Deprez (UGent) , Freddy Haesebrouck (UGent) and Filip Boyen (UGent)
Author
Organization
Abstract
Fast and accurate identification of Mycoplasma bovis in cattle samples is of great importance for rational treatment and control of pneumonia, arthritis and mastitis. However, which growth conditions will allow the fastest identification of M. bovis with MALDI-TOF MS remains unclear. Therefore, growth conditions and incubation time were investigated to optimize identification of M. bovis with MALDI-TOF MS and an in-house library was constructed. Nine different M. bovis strains were inoculated in triplicate in three liquid media (B1-3). Basic broth (B1) consisted of pleuropneumonia-like organism broth, enriched with 25% horse serum and 0.7% yeast extract. B2 and B3 were additionally supplemented with 0.5% pyruvate or 520 mu g/mL ampicillin, respectively. Protein extraction was performed after 24, 48, 72, 96 and 120 h of incubation (37 degrees C, 5% CO2) and processed with Autoflex III smartbeam. Identification scores >= 1.7 were interpreted as reliable. The present study showed reliable identification of M. bovis with MALDI-TOF MS as early as 24 h after inoculation, and in broth supplemented with pyruvate, up to 120 h after inoculation. Serial dilutions showed improved survival of M. bovis in broth with pyruvate. The addition of ampicillin to prevent contamination, did not impair identification of M. bovis and state-of-the-art in-house libraries contributed to higher identification scores for M. bovis with MALDI-TOF MS.
Keywords
Ampicillin, Incubation time, Library, Protein extraction, Pyruvate, DESORPTION IONIZATION-TIME, FLIGHT MASS-SPECTROMETRY, RESPIRATORY-DISEASE, INFECTIONS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 204.37 KB

Citation

Please use this url to cite or link to this publication:

MLA
Bokma, Jade, et al. “Optimizing Identification of Mycoplasma Bovis by MALDI-TOF MS.” RESEARCH IN VETERINARY SCIENCE, vol. 125, 2019, pp. 185–88.
APA
Bokma, J., Pardon, B., Van Driessche, L., Gille, L., Deprez, P., Haesebrouck, F., & Boyen, F. (2019). Optimizing identification of Mycoplasma bovis by MALDI-TOF MS. RESEARCH IN VETERINARY SCIENCE, 125, 185–188.
Chicago author-date
Bokma, Jade, Bart Pardon, Laura Van Driessche, Linde Gille, Piet Deprez, Freddy Haesebrouck, and Filip Boyen. 2019. “Optimizing Identification of Mycoplasma Bovis by MALDI-TOF MS.” RESEARCH IN VETERINARY SCIENCE 125: 185–88.
Chicago author-date (all authors)
Bokma, Jade, Bart Pardon, Laura Van Driessche, Linde Gille, Piet Deprez, Freddy Haesebrouck, and Filip Boyen. 2019. “Optimizing Identification of Mycoplasma Bovis by MALDI-TOF MS.” RESEARCH IN VETERINARY SCIENCE 125: 185–188.
Vancouver
1.
Bokma J, Pardon B, Van Driessche L, Gille L, Deprez P, Haesebrouck F, et al. Optimizing identification of Mycoplasma bovis by MALDI-TOF MS. RESEARCH IN VETERINARY SCIENCE. 2019;125:185–8.
IEEE
[1]
J. Bokma et al., “Optimizing identification of Mycoplasma bovis by MALDI-TOF MS,” RESEARCH IN VETERINARY SCIENCE, vol. 125, pp. 185–188, 2019.
@article{8620793,
  abstract     = {Fast and accurate identification of Mycoplasma bovis in cattle samples is of great importance for rational treatment and control of pneumonia, arthritis and mastitis. However, which growth conditions will allow the fastest identification of M. bovis with MALDI-TOF MS remains unclear. Therefore, growth conditions and incubation time were investigated to optimize identification of M. bovis with MALDI-TOF MS and an in-house library was constructed. Nine different M. bovis strains were inoculated in triplicate in three liquid media (B1-3). Basic broth (B1) consisted of pleuropneumonia-like organism broth, enriched with 25% horse serum and 0.7% yeast extract. B2 and B3 were additionally supplemented with 0.5% pyruvate or 520 mu g/mL ampicillin, respectively. Protein extraction was performed after 24, 48, 72, 96 and 120 h of incubation (37 degrees C, 5% CO2) and processed with Autoflex III smartbeam. Identification scores >= 1.7 were interpreted as reliable. The present study showed reliable identification of M. bovis with MALDI-TOF MS as early as 24 h after inoculation, and in broth supplemented with pyruvate, up to 120 h after inoculation. Serial dilutions showed improved survival of M. bovis in broth with pyruvate. The addition of ampicillin to prevent contamination, did not impair identification of M. bovis and state-of-the-art in-house libraries contributed to higher identification scores for M. bovis with MALDI-TOF MS.},
  author       = {Bokma, Jade and Pardon, Bart and Van Driessche, Laura and Gille, Linde and Deprez, Piet and Haesebrouck, Freddy and Boyen, Filip},
  issn         = {0034-5288},
  journal      = {RESEARCH IN VETERINARY SCIENCE},
  keywords     = {Ampicillin,Incubation time,Library,Protein extraction,Pyruvate,DESORPTION IONIZATION-TIME,FLIGHT MASS-SPECTROMETRY,RESPIRATORY-DISEASE,INFECTIONS},
  language     = {eng},
  pages        = {185--188},
  title        = {Optimizing identification of Mycoplasma bovis by MALDI-TOF MS},
  url          = {http://dx.doi.org/10.1016/j.rvsc.2019.06.010},
  volume       = {125},
  year         = {2019},
}

Altmetric
View in Altmetric
Web of Science
Times cited: