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Analysis of spatio-temporal fungal growth dynamics under different environmental conditions

(2019) IMA FUNGUS. 10.
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Abstract
Traditionally, fungal growth dynamics were assessed manually, limiting the research to a few environmental conditions and/or fungal species. Fortunately, more automated ways of measurement are gaining momentum due to the availability of cheap imaging and processing equipment and the development of dedicated image analysis algorithms. In this paper, we use image analysis to assess the impact of environmental conditions on the growth dynamics of two economically important fungal species, Coniophora puteana and Rhizoctonia solani. Sixteen environmental conditions combining four temperatures (15, 20, 25 and 30°C) and four relative humidity (RH) conditions (65, 70, 75 and 80% RH) were tested. Fungal growth characteristics were extracted from images of the growing fungi, taken at regular points in time. Advanced time series analysis was applied to quantitatively compare the effect of the environmental conditions on these growth characteristics. The evolution of the mycelial area and the number of tips over time resulted in typical sigmoidal growth curves. Other growth characteristics such as the mean hyphal segment length did not vary significantly over time. Temperature and RH usually had a combined effect on the growth dynamics of the mycelial area and the number of tips. When defining optimal growth conditions for a fungus, it is therefore of primordial importance that the effect of temperature and RH is assessed simultaneously. At the most extreme conditions we tested, the mycelium most probably experienced water stress when developing over the inert Petri dish surface. An RH of 65% (independent of temperature) for C. puteana and a temperature of 30 °C (independent of RH) for both C. puteana and R. solani therefore always resulted in limited fungal growth, while the optimal growing conditions were at 20 °C and 75% RH and at 25°C and 80% RH for R. solani and at 20°C and 75% RH for C. puteana. The method applied in this study offers an updated and broader alternative to classical and narrowly focused studies on fungal growth dynamics, and is well suited to efficiently assess the effect of environmental conditions on fungal growth.
Keywords
Time series analysis, Temperature, Relative humidity, Fungal networks, Coniophora puteana, Rhizoctonia solani, RHIZOCTONIA-SOLANI, RELATIVE-HUMIDITY, WATER ACTIVITY, ANASTOMOSIS GROUPS, BUILDING-MATERIALS, SPORE GERMINATION, MYCELIUM GROWTH, HYPHAL GROWTH, TEMPERATURE, WOOD

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MLA
De Ligne, Liselotte, et al. “Analysis of Spatio-Temporal Fungal Growth Dynamics under Different Environmental Conditions.” IMA FUNGUS, vol. 10, 2019.
APA
De Ligne, L., Vidal Diez de Ulzurrun, G., Baetens, J., Van den Bulcke, J., Van Acker, J., & De Baets, B. (2019). Analysis of spatio-temporal fungal growth dynamics under different environmental conditions. IMA FUNGUS, 10.
Chicago author-date
De Ligne, Liselotte, Guillermo Vidal Diez de Ulzurrun, Jan Baetens, Jan Van den Bulcke, Joris Van Acker, and Bernard De Baets. 2019. “Analysis of Spatio-Temporal Fungal Growth Dynamics under Different Environmental Conditions.” IMA FUNGUS 10.
Chicago author-date (all authors)
De Ligne, Liselotte, Guillermo Vidal Diez de Ulzurrun, Jan Baetens, Jan Van den Bulcke, Joris Van Acker, and Bernard De Baets. 2019. “Analysis of Spatio-Temporal Fungal Growth Dynamics under Different Environmental Conditions.” IMA FUNGUS 10.
Vancouver
1.
De Ligne L, Vidal Diez de Ulzurrun G, Baetens J, Van den Bulcke J, Van Acker J, De Baets B. Analysis of spatio-temporal fungal growth dynamics under different environmental conditions. IMA FUNGUS. 2019;10.
IEEE
[1]
L. De Ligne, G. Vidal Diez de Ulzurrun, J. Baetens, J. Van den Bulcke, J. Van Acker, and B. De Baets, “Analysis of spatio-temporal fungal growth dynamics under different environmental conditions,” IMA FUNGUS, vol. 10, 2019.
@article{8620089,
  abstract     = {Traditionally, fungal growth dynamics were assessed manually, limiting the research to a few environmental conditions and/or fungal species. Fortunately, more automated ways of measurement are gaining momentum due to the availability of cheap imaging and processing equipment and the development of dedicated image analysis algorithms. In this paper, we use image analysis to assess the impact of environmental conditions on the growth dynamics of two economically important fungal species, Coniophora puteana and Rhizoctonia solani. Sixteen environmental conditions combining four temperatures (15, 20, 25 and 30°C) and four relative humidity (RH) conditions (65, 70, 75 and 80% RH) were tested. Fungal growth characteristics were extracted from images of the growing fungi, taken at regular points in time. Advanced time series analysis was applied to quantitatively compare the effect of the environmental conditions on these growth characteristics. The evolution of the mycelial area and the number of tips over time resulted in typical sigmoidal growth curves. Other growth characteristics such as the mean hyphal segment length did not vary significantly over time. Temperature and RH usually had a combined effect on the growth dynamics of the mycelial area and the number of tips. When defining optimal growth conditions for a fungus, it is therefore of primordial importance that the effect of temperature and RH is assessed simultaneously. At the most extreme conditions we tested, the mycelium most probably experienced water stress when developing over the inert Petri dish surface. An RH of 65% (independent of temperature) for C. puteana and a temperature of 30 °C (independent of RH) for both C. puteana and R. solani therefore always resulted in limited fungal growth, while the optimal growing conditions were at 20 °C and 75% RH and at 25°C and 80% RH for R. solani and at 20°C and 75% RH for C. puteana. The method applied in this study offers an updated and broader alternative to classical and narrowly focused studies on fungal growth dynamics, and is well suited to efficiently assess the effect of environmental conditions on fungal growth.},
  articleno    = {7},
  author       = {De Ligne, Liselotte and Vidal Diez de Ulzurrun, Guillermo and Baetens, Jan and Van den Bulcke, Jan and Van Acker, Joris and De Baets, Bernard},
  issn         = {2210-6340},
  journal      = {IMA FUNGUS},
  keywords     = {Time series analysis,Temperature,Relative humidity,Fungal networks,Coniophora puteana,Rhizoctonia solani,RHIZOCTONIA-SOLANI,RELATIVE-HUMIDITY,WATER ACTIVITY,ANASTOMOSIS GROUPS,BUILDING-MATERIALS,SPORE GERMINATION,MYCELIUM GROWTH,HYPHAL GROWTH,TEMPERATURE,WOOD},
  language     = {eng},
  pages        = {13},
  title        = {Analysis of spatio-temporal fungal growth dynamics under different environmental conditions},
  url          = {http://dx.doi.org/10.1186/s43008-019-0009-3},
  volume       = {10},
  year         = {2019},
}

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