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Selective control of SIRP-α-positive airway dendritic cell trafficking through CD47 is critical for the development of TH2-mediated allergic inflammation

M Raymond, M Rubio, G Fortin, KH Shalaby, Hamida Hammad UGent, Bart Lambrecht UGent and M Sarfati (2009) JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. 124(6). p.1333-1342
abstract
Background: Dendritic cells (DCs) are essential for the initiation and maintenance of T(H)2 responses to inhaled antigen that lead to the establishment of allergic diseases. Two subpopulations of nonplasmacytoid DCs (ie, CD11b(low)CD103(+) and CD11b(high)CD103(-)) are found in lung/airway tissues. Yet the identification and migratory properties of the DC subset that contributes to T(H)2-mediated responses remain to be clarified. CD47, a signal regulatory protein (SIRP)-alpha partner, reportedly governed skin DC migration. Objective: We here thought to investigate the role of CD47/SIRP-alpha interactions in airway DC trafficking and the development of allergic airway inflammation. Methods: We characterized the DC influx into lungs and mediastinal lymph nodes in CD47(-/-) and CD47(+/+) BALB/c mice by using experimental models of allergic asthma. Mice were systemically (intraperitoneal ovalbumin/alum) or locally (intratracheal ovalbumin-loaded bone marrow-derived DCs) immunized and challenged by ovalbumin aerosol. We also evaluated the consequences of SIRP-alpha-Fc fusion molecule administration on the induction of airway disease in BALB/c mice. Results: SIRP-alpha selectively identified the CD11b(high)CD103(-) DC subset that predominantly accumulated in mediastinal lymph nodes during airway inflammation. However, CD103(-)SIRP-alpha(+) DC trafficking, T(H)2 responses, and airway disease were impaired in CD47(-/-) mice. Importantly, the adoptive transfer of CD103(-) SIRP-alpha(+)CD47(+/+) but not CD47(-/-) DCs elicited a strong T(H)2 response in CD47(-/-) mice. Finally, the administration of SIRP-alpha-Fc molecule protected BALB/c mice from allergic airway inflammation. Conclusion: Lung CD11b(high)CD103(-)SIRP-alpha(+) DC migration is governed by self-CD47 expression, and manipulation of the CD47/SIRP-alpha pathway suppresses CD103(-)SIRP-alpha(+) DC-driven pathogenic T(H)2 responses and airway inflammation.
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author
organization
alternative title
Selective control of SIRP-alpha-positive airway dendritic cell trafficking through CD47 is critical for the development of T(H)2-mediated allergic inflammation
year
type
journalArticle (original)
publication status
published
subject
keyword
Dendritic cells, migration, CD103, allergic inflammation, T(H)2, CD47, SIRP-alpha, LUNG, ASTHMA, BRONCHIAL LYMPH-NODE, RECEPTOR AGONIST FTY720, IMMUNE-RESPONSE, T-CELLS, ADAPTIVE IMMUNITY, IN-VIVO, INHALED ANTIGEN, MIGRATION
journal title
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
J. Allergy Clin. Immunol.
volume
124
issue
6
pages
1333 - 1342
Web of Science type
Article
Web of Science id
000273071500028
JCR category
ALLERGY
JCR impact factor
9.165 (2009)
JCR rank
1/21 (2009)
JCR quartile
1 (2009)
ISSN
0091-6749
DOI
10.1016/j.jaci.2009.07.021
language
English
UGent publication?
yes
classification
A1
id
861988
handle
http://hdl.handle.net/1854/LU-861988
date created
2010-02-15 14:09:16
date last changed
2017-02-28 10:20:45
@article{861988,
  abstract     = {Background: Dendritic cells (DCs) are essential for the initiation and maintenance of T(H)2 responses to inhaled antigen that lead to the establishment of allergic diseases. Two subpopulations of nonplasmacytoid DCs (ie, CD11b(low)CD103(+) and CD11b(high)CD103(-)) are found in lung/airway tissues. Yet the identification and migratory properties of the DC subset that contributes to T(H)2-mediated responses remain to be clarified. CD47, a signal regulatory protein (SIRP)-alpha partner, reportedly governed skin DC migration. 
Objective: We here thought to investigate the role of CD47/SIRP-alpha interactions in airway DC trafficking and the development of allergic airway inflammation. 
Methods: We characterized the DC influx into lungs and mediastinal lymph nodes in CD47(-/-) and CD47(+/+) BALB/c mice by using experimental models of allergic asthma. Mice were systemically (intraperitoneal ovalbumin/alum) or locally (intratracheal ovalbumin-loaded bone marrow-derived DCs) immunized and challenged by ovalbumin aerosol. We also evaluated the consequences of SIRP-alpha-Fc fusion molecule administration on the induction of airway disease in BALB/c mice. 
Results: SIRP-alpha selectively identified the CD11b(high)CD103(-) DC subset that predominantly accumulated in mediastinal lymph nodes during airway inflammation. However, CD103(-)SIRP-alpha(+) DC trafficking, T(H)2 responses, and airway disease were impaired in CD47(-/-) mice. Importantly, the adoptive transfer of CD103(-) SIRP-alpha(+)CD47(+/+) but not CD47(-/-) DCs elicited a strong T(H)2 response in CD47(-/-) mice. Finally, the administration of SIRP-alpha-Fc molecule protected BALB/c mice from allergic airway inflammation. 
Conclusion: Lung CD11b(high)CD103(-)SIRP-alpha(+) DC migration is governed by self-CD47 expression, and manipulation of the CD47/SIRP-alpha pathway suppresses CD103(-)SIRP-alpha(+) DC-driven pathogenic T(H)2 responses and airway inflammation.},
  author       = {Raymond, M and Rubio, M and Fortin, G and Shalaby, KH and Hammad, Hamida and Lambrecht, Bart and Sarfati, M},
  issn         = {0091-6749},
  journal      = {JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY},
  keyword      = {Dendritic cells,migration,CD103,allergic inflammation,T(H)2,CD47,SIRP-alpha,LUNG,ASTHMA,BRONCHIAL LYMPH-NODE,RECEPTOR AGONIST FTY720,IMMUNE-RESPONSE,T-CELLS,ADAPTIVE IMMUNITY,IN-VIVO,INHALED ANTIGEN,MIGRATION},
  language     = {eng},
  number       = {6},
  pages        = {1333--1342},
  title        = {Selective control of SIRP-\ensuremath{\alpha}-positive airway dendritic cell trafficking through CD47 is critical for the development of TH2-mediated allergic inflammation},
  url          = {http://dx.doi.org/10.1016/j.jaci.2009.07.021},
  volume       = {124},
  year         = {2009},
}

Chicago
Raymond, M, M Rubio, G Fortin, KH Shalaby, Hamida Hammad, Bart Lambrecht, and M Sarfati. 2009. “Selective Control of SIRP-α-positive Airway Dendritic Cell Trafficking Through CD47 Is Critical for the Development of TH2-mediated Allergic Inflammation.” Journal of Allergy and Clinical Immunology 124 (6): 1333–1342.
APA
Raymond, M., Rubio, M., Fortin, G., Shalaby, K., Hammad, H., Lambrecht, B., & Sarfati, M. (2009). Selective control of SIRP-α-positive airway dendritic cell trafficking through CD47 is critical for the development of TH2-mediated allergic inflammation. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 124(6), 1333–1342.
Vancouver
1.
Raymond M, Rubio M, Fortin G, Shalaby K, Hammad H, Lambrecht B, et al. Selective control of SIRP-α-positive airway dendritic cell trafficking through CD47 is critical for the development of TH2-mediated allergic inflammation. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. 2009;124(6):1333–42.
MLA
Raymond, M, M Rubio, G Fortin, et al. “Selective Control of SIRP-α-positive Airway Dendritic Cell Trafficking Through CD47 Is Critical for the Development of TH2-mediated Allergic Inflammation.” JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY 124.6 (2009): 1333–1342. Print.