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Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java

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Abstract
Discriminating between d-tartrate fermenting and non-fermenting strains of Salmonella enterica subsp. enterica serotype Paratyphi B is of major importance as these two variants have different pathogenic profiles. While d-tartrate non-fermenting S. Paratyphi B isolates are the causative agent of typhoid-like fever, d-tartrate fermenting isolates (also called variant Java) of the same serotype trigger the less dangerous gastroenteritis. The determination of S. Paratyphi B variants requires a time-consuming process and complex biochemical tests. Therefore, a quadruplex real-time PCR method, based on the allelic discrimination of molecular markers selected from the scientific literature and from whole genome sequencing data produced in-house, was developed in this study, to be applied to Salmonella isolates. This method was validated with the analysis of 178 S. Paratyphi B (d-tartrate fermenting and non-fermenting) and other serotypes reaching an accuracy, compared with the classical methods, of 98% for serotyping by slide agglutination and 100% for replacement of the biochemical test. The developed real-time PCR permits to save time and to obtain an accurate identification of a S. Paratyphi B serotype and its d-tartrate fermenting profile, which is needed in routine laboratories for fast and efficient diagnostics.
Keywords
DISCRIMINATION, SYSTEM, Salmonella, Paratyphi B, d-tartrate, Real-time PCR, Identification, WGS

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Chicago
Gand, Mathieu, Wesley Mattheus, Assia Saltykova, Nancy Roosens, Katelijne Dierick, Kathleen Marchal, Sigrid C. J. De Keersmaecker, and Sophie Bertrand. 2019. “Development of a Real-time PCR Method for the Genoserotyping of Salmonella Paratyphi B Variant Java.” Applied Microbiology and Biotechnology 103 (12): 4987–4996.
APA
Gand, M., Mattheus, W., Saltykova, A., Roosens, N., Dierick, K., Marchal, K., De Keersmaecker, S. C. J., et al. (2019). Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 103(12), 4987–4996.
Vancouver
1.
Gand M, Mattheus W, Saltykova A, Roosens N, Dierick K, Marchal K, et al. Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. New york: Springer; 2019;103(12):4987–96.
MLA
Gand, Mathieu et al. “Development of a Real-time PCR Method for the Genoserotyping of Salmonella Paratyphi B Variant Java.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103.12 (2019): 4987–4996. Print.
@article{8619127,
  abstract     = {Discriminating between d-tartrate fermenting and non-fermenting strains of Salmonella enterica subsp. enterica serotype Paratyphi B is of major importance as these two variants have different pathogenic profiles. While d-tartrate non-fermenting S. Paratyphi B isolates are the causative agent of typhoid-like fever, d-tartrate fermenting isolates (also called variant Java) of the same serotype trigger the less dangerous gastroenteritis. The determination of S. Paratyphi B variants requires a time-consuming process and complex biochemical tests. Therefore, a quadruplex real-time PCR method, based on the allelic discrimination of molecular markers selected from the scientific literature and from whole genome sequencing data produced in-house, was developed in this study, to be applied to Salmonella isolates. This method was validated with the analysis of 178 S. Paratyphi B (d-tartrate fermenting and non-fermenting) and other serotypes reaching an accuracy, compared with the classical methods, of 98% for serotyping by slide agglutination and 100% for replacement of the biochemical test. The developed real-time PCR permits to save time and to obtain an accurate identification of a S. Paratyphi B serotype and its d-tartrate fermenting profile, which is needed in routine laboratories for fast and efficient diagnostics.},
  author       = {Gand, Mathieu and Mattheus, Wesley and Saltykova, Assia and Roosens, Nancy and Dierick, Katelijne and Marchal, Kathleen and De Keersmaecker, Sigrid C. J. and Bertrand, Sophie},
  issn         = {0175-7598},
  journal      = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
  keywords     = {DISCRIMINATION,SYSTEM,Salmonella,Paratyphi B,d-tartrate,Real-time PCR,Identification,WGS},
  language     = {eng},
  number       = {12},
  pages        = {4987--4996},
  publisher    = {Springer},
  title        = {Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java},
  url          = {http://dx.doi.org/10.1007/s00253-019-09854-4},
  volume       = {103},
  year         = {2019},
}

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