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Comparing two techniques for viscosity measurements in poultry feedstuffs : does it render similar conclusions?

(2018) POULTRY SCIENCE. 97(9). p.3138-3148
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Abstract
Viscosity of intestinal contents is known to affect digestion and absorption of nutrients. In most poultry studies, intestinal viscosity has been measured only after complete removal of solid particles by centrifugation. Centrifugation may however remove particles that contribute to viscosity, hence giving rise to an underestimation of viscosity. Two viscosity measurement techniques, one including a centrifugation step (Brookfield) and the other without (Haake), were compared in-vitro to assess whether both techniques result in similar conclusions regarding viscosity in feedstuffs. Two sets of feedstuff preparations were used. The first set was prepared with different combinations of milled feedstuffs in order to have a wide range of viscosity: 100% corn, 25% corn + 75% wheat, 100% wheat, 90% wheat + 10% rye, all mixed with distilled water. In the second set, barley was incubated with different beta-glucanases, and soybean and sunflower meal were incubated with different pectinases, again all mixed with distilled water. Viscosity was assessed using both techniques (Haake and Brookfield equipments) at six different time points. To evaluate the extent of agreement between the two methods, the Lin's concordance correlation coefficient (CCC) was assessed using the percentage of increase in viscosity within each method, based on pairwise feedstuffs comparison (first set), or relative to the feedstuff without enzyme (second set). The rate of the agreement between the two methods was substantial for the first set of feedstuffs (66%) and for the barley diets incubated with beta-glucanases (69%), whereas the CCC score for the soybean meal diets was very poor (2%) and fair for the sunflower meal diets, incubated with pectinases (32%). The lack of agreement for the latter can be explained by the limited variation in viscosity in these low-viscous mixtures. Although the two techniques are considerably different (e.g., with or without preceding particle removal), they seem to render similar conclusions when applied to poultry feedstuffs to identify distinct differences under the tested circumstances.
Keywords
poultry feedstuffs, viscosity measurement, centrifugation, Haake, Brookfield, BROILER-CHICKENS, BETA-MANNANASE, PASSAGE RATE, PARTICLES, BEHAVIOR, ENZYMES, GROWTH, TRACT

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Citation

Please use this url to cite or link to this publication:

MLA
Khadem, Alireza et al. “Comparing Two Techniques for Viscosity Measurements in Poultry Feedstuffs : Does It Render Similar Conclusions?” POULTRY SCIENCE 97.9 (2018): 3138–3148. Print.
APA
Khadem, A., Ribeiro Alves Lourenço, M., Delezie, E., Maertens, L., Goderis, A., Mombaerts, R., & Janssens, G. (2018). Comparing two techniques for viscosity measurements in poultry feedstuffs : does it render similar conclusions? POULTRY SCIENCE, 97(9), 3138–3148.
Chicago author-date
Khadem, Alireza, Marta Ribeiro Alves Lourenço, Evelyne Delezie, Luc Maertens, Anne Goderis, Ronny Mombaerts, and Geert Janssens. 2018. “Comparing Two Techniques for Viscosity Measurements in Poultry Feedstuffs : Does It Render Similar Conclusions?” Poultry Science 97 (9): 3138–3148.
Chicago author-date (all authors)
Khadem, Alireza, Marta Ribeiro Alves Lourenço, Evelyne Delezie, Luc Maertens, Anne Goderis, Ronny Mombaerts, and Geert Janssens. 2018. “Comparing Two Techniques for Viscosity Measurements in Poultry Feedstuffs : Does It Render Similar Conclusions?” Poultry Science 97 (9): 3138–3148.
Vancouver
1.
Khadem A, Ribeiro Alves Lourenço M, Delezie E, Maertens L, Goderis A, Mombaerts R, et al. Comparing two techniques for viscosity measurements in poultry feedstuffs : does it render similar conclusions? POULTRY SCIENCE. 2018;97(9):3138–48.
IEEE
[1]
A. Khadem et al., “Comparing two techniques for viscosity measurements in poultry feedstuffs : does it render similar conclusions?,” POULTRY SCIENCE, vol. 97, no. 9, pp. 3138–3148, 2018.
@article{8618653,
  abstract     = {Viscosity of intestinal contents is known to affect digestion and absorption of nutrients. In most poultry studies, intestinal viscosity has been measured only after complete removal of solid particles by centrifugation. Centrifugation may however remove particles that contribute to viscosity, hence giving rise to an underestimation of viscosity. Two viscosity measurement techniques, one including a centrifugation step (Brookfield) and the other without (Haake), were compared in-vitro to assess whether both techniques result in similar conclusions regarding viscosity in feedstuffs. Two sets of feedstuff preparations were used. The first set was prepared with different combinations of milled feedstuffs in order to have a wide range of viscosity: 100% corn, 25% corn + 75% wheat, 100% wheat, 90% wheat + 10% rye, all mixed with distilled water. In the second set, barley was incubated with different beta-glucanases, and soybean and sunflower meal were incubated with different pectinases, again all mixed with distilled water. Viscosity was assessed using both techniques (Haake and Brookfield equipments) at six different time points. To evaluate the extent of agreement between the two methods, the Lin's concordance correlation coefficient (CCC) was assessed using the percentage of increase in viscosity within each method, based on pairwise feedstuffs comparison (first set), or relative to the feedstuff without enzyme (second set). The rate of the agreement between the two methods was substantial for the first set of feedstuffs (66%) and for the barley diets incubated with beta-glucanases (69%), whereas the CCC score for the soybean meal diets was very poor (2%) and fair for the sunflower meal diets, incubated with pectinases (32%). The lack of agreement for the latter can be explained by the limited variation in viscosity in these low-viscous mixtures. Although the two techniques are considerably different (e.g., with or without preceding particle removal), they seem to render similar conclusions when applied to poultry feedstuffs to identify distinct differences under the tested circumstances.},
  author       = {Khadem, Alireza and Ribeiro Alves Lourenço, Marta and Delezie, Evelyne and Maertens, Luc and Goderis, Anne and Mombaerts, Ronny and Janssens, Geert},
  issn         = {0032-5791},
  journal      = {POULTRY SCIENCE},
  keywords     = {poultry feedstuffs,viscosity measurement,centrifugation,Haake,Brookfield,BROILER-CHICKENS,BETA-MANNANASE,PASSAGE RATE,PARTICLES,BEHAVIOR,ENZYMES,GROWTH,TRACT},
  language     = {eng},
  number       = {9},
  pages        = {3138--3148},
  title        = {Comparing two techniques for viscosity measurements in poultry feedstuffs : does it render similar conclusions?},
  url          = {http://dx.doi.org/10.3382/ps/pey190},
  volume       = {97},
  year         = {2018},
}

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