The N-glycome of the hemipteran pest insect Nilaparvata lugens reveals unexpected sex differences
- Author
- Freja Scheys, Kristof De Schutter (UGent) , Ying Shen (UGent) , Na Yu (UGent) , Nicolas Smargiasso, Edwin De Pauw, Els Van Damme (UGent) and Guy Smagghe (UGent)
- Organization
- Abstract
- The brown planthopper, Nilaparvata lugens, is a model species for hemimetabolous development and the most important pest insect in rice, which is the major staple crop for about half of the world population. Despite its importance, little is known of the N-glycosylation process in this insect. Here we report on the N-glycome for the post-embryonic stages of N. lugens, revealing unique features that are different from the holometabolous insect models, as the fruit fly Drosophila melanogaster and the beetle Tribolium castaneum. Analysis of the N-glycan fingerprint for male and female adults showed sex-specific N-glycosylation in insects. Specifically, the female adults progress towards a unique glycan profile with a striking increase in high mannose N-glycans. The N-glycome of N. lugens contributes to study pathways differentiating between sexes, and the results shed light on the evolution and differences in development between primitive hemimetabolous insects and more advanced holometabolous insects. The data are discussed in relation to potential function(s) in development and sex specificity.
- Keywords
- N-Glycosylation, Pest insect, Nilaparvata lugens, Mass spectrometry, qPCR, DROSOPHILA-MELANOGASTER, LINKED CARBOHYDRATE, GLYCOSYLATION, GLYCANS, GLYCOBIOLOGY, SILKWORM, RNAI
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8618237
- MLA
- Scheys, Freja, et al. “The N-Glycome of the Hemipteran Pest Insect Nilaparvata Lugens Reveals Unexpected Sex Differences.” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 107, 2019, pp. 39–45, doi:10.1016/j.ibmb.2019.01.008.
- APA
- Scheys, F., De Schutter, K., Shen, Y., Yu, N., Smargiasso, N., De Pauw, E., … Smagghe, G. (2019). The N-glycome of the hemipteran pest insect Nilaparvata lugens reveals unexpected sex differences. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 107, 39–45. https://doi.org/10.1016/j.ibmb.2019.01.008
- Chicago author-date
- Scheys, Freja, Kristof De Schutter, Ying Shen, Na Yu, Nicolas Smargiasso, Edwin De Pauw, Els Van Damme, and Guy Smagghe. 2019. “The N-Glycome of the Hemipteran Pest Insect Nilaparvata Lugens Reveals Unexpected Sex Differences.” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 107: 39–45. https://doi.org/10.1016/j.ibmb.2019.01.008.
- Chicago author-date (all authors)
- Scheys, Freja, Kristof De Schutter, Ying Shen, Na Yu, Nicolas Smargiasso, Edwin De Pauw, Els Van Damme, and Guy Smagghe. 2019. “The N-Glycome of the Hemipteran Pest Insect Nilaparvata Lugens Reveals Unexpected Sex Differences.” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 107: 39–45. doi:10.1016/j.ibmb.2019.01.008.
- Vancouver
- 1.Scheys F, De Schutter K, Shen Y, Yu N, Smargiasso N, De Pauw E, et al. The N-glycome of the hemipteran pest insect Nilaparvata lugens reveals unexpected sex differences. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY. 2019;107:39–45.
- IEEE
- [1]F. Scheys et al., “The N-glycome of the hemipteran pest insect Nilaparvata lugens reveals unexpected sex differences,” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 107, pp. 39–45, 2019.
@article{8618237,
abstract = {{The brown planthopper, Nilaparvata lugens, is a model species for hemimetabolous development and the most important pest insect in rice, which is the major staple crop for about half of the world population. Despite its importance, little is known of the N-glycosylation process in this insect. Here we report on the N-glycome for the post-embryonic stages of N. lugens, revealing unique features that are different from the holometabolous insect models, as the fruit fly Drosophila melanogaster and the beetle Tribolium castaneum. Analysis of the N-glycan fingerprint for male and female adults showed sex-specific N-glycosylation in insects. Specifically, the female adults progress towards a unique glycan profile with a striking increase in high mannose N-glycans. The N-glycome of N. lugens contributes to study pathways differentiating between sexes, and the results shed light on the evolution and differences in development between primitive hemimetabolous insects and more advanced holometabolous insects. The data are discussed in relation to potential function(s) in development and sex specificity.}},
author = {{Scheys, Freja and De Schutter, Kristof and Shen, Ying and Yu, Na and Smargiasso, Nicolas and De Pauw, Edwin and Van Damme, Els and Smagghe, Guy}},
issn = {{0965-1748}},
journal = {{INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}},
keywords = {{N-Glycosylation,Pest insect,Nilaparvata lugens,Mass spectrometry,qPCR,DROSOPHILA-MELANOGASTER,LINKED CARBOHYDRATE,GLYCOSYLATION,GLYCANS,GLYCOBIOLOGY,SILKWORM,RNAI}},
language = {{eng}},
pages = {{39--45}},
title = {{The N-glycome of the hemipteran pest insect Nilaparvata lugens reveals unexpected sex differences}},
url = {{http://dx.doi.org/10.1016/j.ibmb.2019.01.008}},
volume = {{107}},
year = {{2019}},
}
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