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High-resolution in vivo imaging of xylem-transported CO2 in leaves based on real-time 11C-tracing

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Abstract
Plant studies using the short-lived isotope C-11 to label photosynthate via atmospheric carbon dioxide (CO2), have greatly advanced our knowledge about the allocation of recent photosynthate from leaves to sinks. However, a second source for photosynthesis is CO2 in the transpiration stream, coming from respiration in plant tissues. Here, we use in vivo tracing of xylem-transported (CO2)-C-11 to increase our knowledge on whole plant carbon cycling.We developed a newmethod for in vivo tracing of xylem-transported CO2 in excised poplar leaves using C-11 in combination with positron emission tomography (PET) and autoradiography. To show the applicability of both measurement techniques in visualizing and quantifying CO2 transport dynamics, we administered the tracer via the cut petiole and manipulated the transport by excluding light or preventing transpiration. Irrespective of manipulation, some tracer was found in main and secondary veins, little of it was fixed in minor veins or mesophyll, while most of it diffused out the leaf. Transpiration, phloem loading and CO2 recycling were identified as mechanisms that could be responsible for the transport of internal CO2. Both C-11-PET and autoradiography can be successfully applied to study xylem-transported CO2, toward better understanding of leaf and plant carbon cycling, and its importance in different growing conditions.
Keywords
Populus canadensis, C-11 (carbon-11), isotope, radiotracer, positron emission tomography (PET), positron autoradiography, xylem CO2 transport, WOODY TISSUE PHOTOSYNTHESIS, CARBON ALLOCATION DYNAMICS, DISTANCE TRACER-TRANSPORT, C-13-LABELED CO2, WATER-LOSS, PHLOEM, TRANSPIRATION, C-11, PHOTOASSIMILATE, TRANSLOCATION

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MLA
Hubeau, Michiel, et al. “High-Resolution in Vivo Imaging of Xylem-Transported CO2 in Leaves Based on Real-Time 11C-Tracing.” FRONTIERS IN FORESTS AND GLOBAL CHANGE, vol. 2, 2019, doi:10.3389/ffgc.2019.00025.
APA
Hubeau, M., Thorpe, M. R., Mincke, J., Bloemen, J., Bauweraerts, I., Minchin, P. E. H., … Steppe, K. (2019). High-resolution in vivo imaging of xylem-transported CO2 in leaves based on real-time 11C-tracing. FRONTIERS IN FORESTS AND GLOBAL CHANGE, 2. https://doi.org/10.3389/ffgc.2019.00025
Chicago author-date
Hubeau, Michiel, Michael Robert Thorpe, Jens Mincke, Jasper Bloemen, Ingvar Bauweraerts, Peter Etheridge Havila Minchin, Veerle De Schepper, et al. 2019. “High-Resolution in Vivo Imaging of Xylem-Transported CO2 in Leaves Based on Real-Time 11C-Tracing.” FRONTIERS IN FORESTS AND GLOBAL CHANGE 2. https://doi.org/10.3389/ffgc.2019.00025.
Chicago author-date (all authors)
Hubeau, Michiel, Michael Robert Thorpe, Jens Mincke, Jasper Bloemen, Ingvar Bauweraerts, Peter Etheridge Havila Minchin, Veerle De Schepper, Filip De Vos, Christian Vanhove, Stefaan Vandenberghe, and Kathy Steppe. 2019. “High-Resolution in Vivo Imaging of Xylem-Transported CO2 in Leaves Based on Real-Time 11C-Tracing.” FRONTIERS IN FORESTS AND GLOBAL CHANGE 2. doi:10.3389/ffgc.2019.00025.
Vancouver
1.
Hubeau M, Thorpe MR, Mincke J, Bloemen J, Bauweraerts I, Minchin PEH, et al. High-resolution in vivo imaging of xylem-transported CO2 in leaves based on real-time 11C-tracing. FRONTIERS IN FORESTS AND GLOBAL CHANGE. 2019;2.
IEEE
[1]
M. Hubeau et al., “High-resolution in vivo imaging of xylem-transported CO2 in leaves based on real-time 11C-tracing,” FRONTIERS IN FORESTS AND GLOBAL CHANGE, vol. 2, 2019.
@article{8617789,
  abstract     = {{Plant studies using the short-lived isotope C-11 to label photosynthate via atmospheric carbon dioxide (CO2), have greatly advanced our knowledge about the allocation of recent photosynthate from leaves to sinks. However, a second source for photosynthesis is CO2 in the transpiration stream, coming from respiration in plant tissues. Here, we use in vivo tracing of xylem-transported (CO2)-C-11 to increase our knowledge on whole plant carbon cycling.We developed a newmethod for in vivo tracing of xylem-transported CO2 in excised poplar leaves using C-11 in combination with positron emission tomography (PET) and autoradiography. To show the applicability of both measurement techniques in visualizing and quantifying CO2 transport dynamics, we administered the tracer via the cut petiole and manipulated the transport by excluding light or preventing transpiration. Irrespective of manipulation, some tracer was found in main and secondary veins, little of it was fixed in minor veins or mesophyll, while most of it diffused out the leaf. Transpiration, phloem loading and CO2 recycling were identified as mechanisms that could be responsible for the transport of internal CO2. Both C-11-PET and autoradiography can be successfully applied to study xylem-transported CO2, toward better understanding of leaf and plant carbon cycling, and its importance in different growing conditions.}},
  articleno    = {{25}},
  author       = {{Hubeau, Michiel and Thorpe, Michael Robert and Mincke, Jens and Bloemen, Jasper and Bauweraerts, Ingvar and Minchin, Peter Etheridge Havila and De Schepper, Veerle and De Vos, Filip and Vanhove, Christian and Vandenberghe, Stefaan and Steppe, Kathy}},
  issn         = {{2624-893X}},
  journal      = {{FRONTIERS IN FORESTS AND GLOBAL CHANGE}},
  keywords     = {{Populus canadensis,C-11 (carbon-11),isotope,radiotracer,positron emission tomography (PET),positron autoradiography,xylem CO2 transport,WOODY TISSUE PHOTOSYNTHESIS,CARBON ALLOCATION DYNAMICS,DISTANCE TRACER-TRANSPORT,C-13-LABELED CO2,WATER-LOSS,PHLOEM,TRANSPIRATION,C-11,PHOTOASSIMILATE,TRANSLOCATION}},
  language     = {{eng}},
  pages        = {{12}},
  title        = {{High-resolution in vivo imaging of xylem-transported CO2 in leaves based on real-time 11C-tracing}},
  url          = {{http://dx.doi.org/10.3389/ffgc.2019.00025}},
  volume       = {{2}},
  year         = {{2019}},
}

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