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Targeted genome engineering in Xenopus using the transcription activator-like effector nuclease (TALEN) technology

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Abstract
Targeted genome engineering technologies are revolutionizing the field of functional genomics and have been extensively used in a variety of model organisms, including X. tropicalis and X. laevis. The original methods based on Zn-finger proteins coupled to endonuclease domains were initially replaced by the more efficient and straightforward transcription activator-like effector nucleases (TALENs), adapted from plant pathogenic Xanthomonas species. Although functional genomics are more recently dominated by the even faster and more convenient CRISPR/Cas9 technology, the use of TALENs may still be preferred in a number of cases. We have successfully implemented this technology in Xenopus and in this chapter we describe our working protocol for targeted genome editing in X. tropicalis using TALENs.

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MLA
Van Nieuwenhuysen, Tom, and Kris Vleminckx. “Targeted Genome Engineering in Xenopus Using the Transcription Activator-like Effector Nuclease (TALEN) Technology.” Xenopus : Methods and Protocols. Ed. Kris Vleminckx. Vol. 1865. New York, NY, USA: Springer Humana Press, 2018. 55–65. Print.
APA
Van Nieuwenhuysen, T., & Vleminckx, K. (2018). Targeted genome engineering in Xenopus using the transcription activator-like effector nuclease (TALEN) technology. In K. Vleminckx (Ed.), Xenopus : methods and protocols (Vol. 1865, pp. 55–65). New York, NY, USA: Springer Humana Press.
Chicago author-date
Van Nieuwenhuysen, Tom, and Kris Vleminckx. 2018. “Targeted Genome Engineering in Xenopus Using the Transcription Activator-like Effector Nuclease (TALEN) Technology.” In Xenopus : Methods and Protocols, ed. Kris Vleminckx, 1865:55–65. New York, NY, USA: Springer Humana Press.
Chicago author-date (all authors)
Van Nieuwenhuysen, Tom, and Kris Vleminckx. 2018. “Targeted Genome Engineering in Xenopus Using the Transcription Activator-like Effector Nuclease (TALEN) Technology.” In Xenopus : Methods and Protocols, ed. Kris Vleminckx, 1865:55–65. New York, NY, USA: Springer Humana Press.
Vancouver
1.
Van Nieuwenhuysen T, Vleminckx K. Targeted genome engineering in Xenopus using the transcription activator-like effector nuclease (TALEN) technology. In: Vleminckx K, editor. Xenopus : methods and protocols. New York, NY, USA: Springer Humana Press; 2018. p. 55–65.
IEEE
[1]
T. Van Nieuwenhuysen and K. Vleminckx, “Targeted genome engineering in Xenopus using the transcription activator-like effector nuclease (TALEN) technology,” in Xenopus : methods and protocols, vol. 1865, K. Vleminckx, Ed. New York, NY, USA: Springer Humana Press, 2018, pp. 55–65.
@incollection{8617562,
  abstract     = {{Targeted genome engineering technologies are revolutionizing the field of functional genomics and have been extensively used in a variety of model organisms, including X. tropicalis and X. laevis. The original methods based on Zn-finger proteins coupled to endonuclease domains were initially replaced by the more efficient and straightforward transcription activator-like effector nucleases (TALENs), adapted from plant pathogenic Xanthomonas species. Although functional genomics are more recently dominated by the even faster and more convenient CRISPR/Cas9 technology, the use of TALENs may still be preferred in a number of cases. We have successfully implemented this technology in Xenopus and in this chapter we describe our working protocol for targeted genome editing in X. tropicalis using TALENs.}},
  author       = {{Van Nieuwenhuysen, Tom and Vleminckx, Kris}},
  booktitle    = {{Xenopus : methods and protocols}},
  editor       = {{Vleminckx, Kris}},
  isbn         = {{9781493987832}},
  issn         = {{1064-3745}},
  language     = {{eng}},
  pages        = {{55--65}},
  publisher    = {{Springer Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Targeted genome engineering in Xenopus using the transcription activator-like effector nuclease (TALEN) technology}},
  url          = {{http://dx.doi.org/10.1007/978-1-4939-8784-9_4}},
  volume       = {{1865}},
  year         = {{2018}},
}

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