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TPX2-LIKE PROTEIN 3 is the primary activator of α-Aurora kinases and is essential for embryogenesis

(2019) PLANT PHYSIOLOGY. 180(3). p.1389-1405
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Abstract
Aurora kinases are key regulators of mitosis. Multicellular eukaryotes generally possess two functionally-diverged types of Aurora kinases. In plants, including Arabidopsis thaliana, these are termed α and β Auroras. As the functional specification of Aurora kinases is determined by their specific interaction partners, we initiated interactomics analyses using both Arabidopsis α Aurora kinases (AUR1 and AUR2). Proteomics results revealed that TPX2-LIKE PROTEINS 2 and 3 (TPXL2/3) prominently associated with α Auroras, as did the conserved TPX2 to a lower degree. Like TPX2, TPXL2 and TPXL3 strongly activated the AUR1 kinase but exhibited cell cycle-dependent localization differences on microtubule arrays. The separate functions of TPX2 and TPXL2/3 were also suggested by their different influences on AUR1 localization upon ectopic expressions. Furthermore, genetic analyses showed that TPXL3, but not TPX2 and TPXL2, acts non-redundantly to enable proper embryo development. In contrast to vertebrates, plants have an expanded TPX2 family and these family members have both redundant and unique functions. Moreover, as neither TPXL2 nor TPXL3 contains the C-terminal Kinesin-5 binding domain present in the canonical TPX2, the targeting and activity of this kinesin must be organized differently in plants.

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MLA
Boruc, Joanna, et al. “TPX2-LIKE PROTEIN 3 Is the Primary Activator of α-Aurora Kinases and Is Essential for Embryogenesis.” PLANT PHYSIOLOGY, vol. 180, no. 3, 2019, pp. 1389–405.
APA
Boruc, J., Deng, X.-G., Mylle, E., Besbrugge, N., Van Durme, M., Demidov, D., … Van Damme, D. (2019). TPX2-LIKE PROTEIN 3 is the primary activator of α-Aurora kinases and is essential for embryogenesis. PLANT PHYSIOLOGY, 180(3), 1389–1405.
Chicago author-date
Boruc, Joanna, Xing-Guang Deng, Evelien Mylle, Nienke Besbrugge, Matthias Van Durme, Dmitri Demidov, Eva Dvorak Tomastikova, et al. 2019. “TPX2-LIKE PROTEIN 3 Is the Primary Activator of α-Aurora Kinases and Is Essential for Embryogenesis.” PLANT PHYSIOLOGY 180 (3): 1389–1405.
Chicago author-date (all authors)
Boruc, Joanna, Xing-Guang Deng, Evelien Mylle, Nienke Besbrugge, Matthias Van Durme, Dmitri Demidov, Eva Dvorak Tomastikova, Ton-Reen Connie Tan, Michaël Vandorpe, Dominique Eeckhout, Tom Beeckman, Moritz Nowack, Geert De Jaeger, Honghui Lin, Bo Liu, and Daniël Van Damme. 2019. “TPX2-LIKE PROTEIN 3 Is the Primary Activator of α-Aurora Kinases and Is Essential for Embryogenesis.” PLANT PHYSIOLOGY 180 (3): 1389–1405.
Vancouver
1.
Boruc J, Deng X-G, Mylle E, Besbrugge N, Van Durme M, Demidov D, et al. TPX2-LIKE PROTEIN 3 is the primary activator of α-Aurora kinases and is essential for embryogenesis. PLANT PHYSIOLOGY. 2019;180(3):1389–405.
IEEE
[1]
J. Boruc et al., “TPX2-LIKE PROTEIN 3 is the primary activator of α-Aurora kinases and is essential for embryogenesis,” PLANT PHYSIOLOGY, vol. 180, no. 3, pp. 1389–1405, 2019.
@article{8617322,
  abstract     = {Aurora kinases are key regulators of mitosis. Multicellular eukaryotes generally possess two functionally-diverged types of Aurora kinases. In plants, including Arabidopsis thaliana, these are termed α and β Auroras. As the functional specification of Aurora kinases is determined by their specific interaction partners, we initiated interactomics analyses using both Arabidopsis α Aurora kinases (AUR1 and AUR2). Proteomics results revealed that TPX2-LIKE PROTEINS 2 and 3 (TPXL2/3) prominently associated with α Auroras, as did the conserved TPX2 to a lower degree. Like TPX2, TPXL2 and TPXL3 strongly activated the AUR1 kinase but exhibited cell cycle-dependent localization differences on microtubule arrays. The separate functions of TPX2 and TPXL2/3 were also suggested by their different influences on AUR1 localization upon ectopic expressions. Furthermore, genetic analyses showed that TPXL3, but not TPX2 and TPXL2, acts non-redundantly to enable proper embryo development. In contrast to vertebrates, plants have an expanded TPX2 family and these family members have both redundant and unique functions. Moreover, as neither TPXL2 nor TPXL3 contains the C-terminal Kinesin-5 binding domain present in the canonical TPX2, the targeting and activity of this kinesin must be organized differently in plants.},
  author       = {Boruc, Joanna and Deng, Xing-Guang and Mylle, Evelien and Besbrugge, Nienke and Van Durme, Matthias and Demidov, Dmitri and Tomastikova, Eva Dvorak and Tan, Ton-Reen Connie and Vandorpe, Michaël and Eeckhout, Dominique and Beeckman, Tom and Nowack, Moritz and De Jaeger, Geert and Lin, Honghui and Liu, Bo and Van Damme, Daniël},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  language     = {eng},
  number       = {3},
  pages        = {1389--1405},
  title        = {TPX2-LIKE PROTEIN 3 is the primary activator of α-Aurora kinases and is essential for embryogenesis},
  url          = {http://dx.doi.org/10.1104/pp.18.01515},
  volume       = {180},
  year         = {2019},
}

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