
Peptide enrichment by ion-pair solid-phase extraction
- Author
- Péter Judák (UGent) , Michaël Polet (UGent) , Peter Van Eenoo (UGent) , Aurélie Benoit, Corinne Buisson and Koen Deventer (UGent)
- Organization
- Abstract
- The technique of Solid-Phase Extraction (SPE) is widely used in various fields to concentrate samples and the search for tools to improve recoveries remains of outmost importance. The use of polymer based cartridges has become prevailing in a broad range of fields to enrich peptides from biological matrices. However, the existing SPE protocols are characterized by disparity. Ion-pairing (IP) reagents are commonly used in chromatographic applications, but their combination with SPE is less known. The aim of this study was to evaluate various SPE loading conditions, including the use of IP reagents, to improve the recoveries of nine selected peptide molecules. Control of pH and the use of IP reagents were found to be crucial to improve the enrichment of the peptides, especially cationic peptides, for which an up to ten-fold increase was observed. The practical potential of the presented theoretical findings were verified by employing IP-SPE for the development of an efficient extraction method for the doping relevant peptide Synacthen. The general proof of principle was obtained by analysis of excretion study urine samples and validation was performed with focus on the limit of detection (20 pg/ml) and recovery (37%).
- Keywords
- Ion-pairing, SPE, Peptides, Oasis HLB, Synacthen, Tetracosactide, ACTH, IMMUNOAFFINITY PURIFICATION, LIQUID-CHROMATOGRAPHY, HUMAN PLASMA, URINE, INSULIN, QUANTIFICATION, VALIDATION, RETENTION, SYNACHTEN, ADDITIVES
Downloads
-
(...).pdf
- full text
- |
- UGent only
- |
- |
- 1.04 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8617195
- MLA
- Judák, Péter, et al. “Peptide Enrichment by Ion-Pair Solid-Phase Extraction.” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, vol. 1121, 2019, pp. 89–95, doi:10.1016/j.jchromb.2019.05.015.
- APA
- Judák, P., Polet, M., Van Eenoo, P., Benoit, A., Buisson, C., & Deventer, K. (2019). Peptide enrichment by ion-pair solid-phase extraction. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1121, 89–95. https://doi.org/10.1016/j.jchromb.2019.05.015
- Chicago author-date
- Judák, Péter, Michaël Polet, Peter Van Eenoo, Aurélie Benoit, Corinne Buisson, and Koen Deventer. 2019. “Peptide Enrichment by Ion-Pair Solid-Phase Extraction.” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1121: 89–95. https://doi.org/10.1016/j.jchromb.2019.05.015.
- Chicago author-date (all authors)
- Judák, Péter, Michaël Polet, Peter Van Eenoo, Aurélie Benoit, Corinne Buisson, and Koen Deventer. 2019. “Peptide Enrichment by Ion-Pair Solid-Phase Extraction.” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1121: 89–95. doi:10.1016/j.jchromb.2019.05.015.
- Vancouver
- 1.Judák P, Polet M, Van Eenoo P, Benoit A, Buisson C, Deventer K. Peptide enrichment by ion-pair solid-phase extraction. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. 2019;1121:89–95.
- IEEE
- [1]P. Judák, M. Polet, P. Van Eenoo, A. Benoit, C. Buisson, and K. Deventer, “Peptide enrichment by ion-pair solid-phase extraction,” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, vol. 1121, pp. 89–95, 2019.
@article{8617195, abstract = {{The technique of Solid-Phase Extraction (SPE) is widely used in various fields to concentrate samples and the search for tools to improve recoveries remains of outmost importance. The use of polymer based cartridges has become prevailing in a broad range of fields to enrich peptides from biological matrices. However, the existing SPE protocols are characterized by disparity. Ion-pairing (IP) reagents are commonly used in chromatographic applications, but their combination with SPE is less known. The aim of this study was to evaluate various SPE loading conditions, including the use of IP reagents, to improve the recoveries of nine selected peptide molecules. Control of pH and the use of IP reagents were found to be crucial to improve the enrichment of the peptides, especially cationic peptides, for which an up to ten-fold increase was observed. The practical potential of the presented theoretical findings were verified by employing IP-SPE for the development of an efficient extraction method for the doping relevant peptide Synacthen. The general proof of principle was obtained by analysis of excretion study urine samples and validation was performed with focus on the limit of detection (20 pg/ml) and recovery (37%).}}, author = {{Judák, Péter and Polet, Michaël and Van Eenoo, Peter and Benoit, Aurélie and Buisson, Corinne and Deventer, Koen}}, issn = {{1570-0232}}, journal = {{JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES}}, keywords = {{Ion-pairing,SPE,Peptides,Oasis HLB,Synacthen,Tetracosactide,ACTH,IMMUNOAFFINITY PURIFICATION,LIQUID-CHROMATOGRAPHY,HUMAN PLASMA,URINE,INSULIN,QUANTIFICATION,VALIDATION,RETENTION,SYNACHTEN,ADDITIVES}}, language = {{eng}}, pages = {{89--95}}, title = {{Peptide enrichment by ion-pair solid-phase extraction}}, url = {{http://dx.doi.org/10.1016/j.jchromb.2019.05.015}}, volume = {{1121}}, year = {{2019}}, }
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: