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Rapid 3D printing of functional nanoparticle-enhanced conduits for effective nerve repair

(2019) ACTA BIOMATERIALIA . 90. p.49-59
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Abstract
Nerve conduits provide an advanced tool for repairing the injured peripheral nerve that often causes disability and mortality. Currently, the efficiency of conduits in repairing peripheral nerve is unsatisfying. Here, we show a functional nanoparticle-enhanced nerve conduit for promoting the regeneration of peripheral nerves. This conduit, which consists of gelatin-methacryloyl (GelMA) hydrogels with drug loaded poly(ethylene glycol)- poly(3-caprolactone) (MPEG-PCL) nanoparticles dispersed in the hydrogel matrix, is rapidly fabricated by a continuous three-dimensional (3D) printing process. While the 3D-printed hydrogel conduit with customized size, shape and structure provides a physical microenvironment for axonal elongation, the nanoparticles sustained release the drug to facilitate the nerve regeneration. The drug, 44(5,10-dimethyl-6-oxo-6,10-dihydro-5H-pyrimido[5,4-b]thieno[3,2-e][1,4]diazepin-2-yl)amino) benzenesulfonamide, is a Hippo pathway inhibitor with multiple functions including improving the proliferation and migration of Schwann cells and up-regulating neurotrophic factors genes. The descried functional nerve conduit efficiently induced the recovery of sciatic injuries in morphology, histopathology and functions in vivo, showing the potential clinical application in peripheral nerve repair. Statements of Significance Functional nerve conduit provides a promising strategy alternative to autografts. In this work, we rapidly customized a nanoparticle-enhanced conduit by the continuous bioprinting process. This nanoparticle in the conduit can release a Hippo pathway inhibitor to facilitate the nerve regeneration and function restoration. The efficacy of the conduits is comparable to that of autograft, suggesting the potential clinical applications. (C) 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Keywords
Biotechnology, Biochemistry, Molecular Biology, Biomaterials, Biomedical Engineering, General Medicine, 3D printing, Nanoparticles, Hydrogel, Peripheral nerve regeneration, Drug delivery, HIPPO PATHWAY, TISSUE HOMEOSTASIS, REGENERATION, STRATEGIES, INJURY, YAP, DELIVERY, GUIDANCE, CELLS, GAP

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Citation

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Chicago
Tao, Jie, Jiumeng Zhang, Ting Du, Xin Xu, Xianming Deng, Shaochen Chen, Jinlu Liu, et al. 2019. “Rapid 3D Printing of Functional Nanoparticle-enhanced Conduits for Effective Nerve Repair.” Acta Biomaterialia 90: 49–59.
APA
Tao, Jie, Zhang, J., Du, T., Xu, X., Deng, X., Chen, S., Liu, J., et al. (2019). Rapid 3D printing of functional nanoparticle-enhanced conduits for effective nerve repair. ACTA BIOMATERIALIA , 90, 49–59.
Vancouver
1.
Tao J, Zhang J, Du T, Xu X, Deng X, Chen S, et al. Rapid 3D printing of functional nanoparticle-enhanced conduits for effective nerve repair. ACTA BIOMATERIALIA . Elsevier ; 2019;90:49–59.
MLA
Tao, Jie et al. “Rapid 3D Printing of Functional Nanoparticle-enhanced Conduits for Effective Nerve Repair.” ACTA BIOMATERIALIA 90 (2019): 49–59. Print.
@article{8617027,
  abstract     = {Nerve conduits provide an advanced tool for repairing the injured peripheral nerve that often causes disability and mortality. Currently, the efficiency of conduits in repairing peripheral nerve is unsatisfying. Here, we show a functional nanoparticle-enhanced nerve conduit for promoting the regeneration of peripheral nerves. This conduit, which consists of gelatin-methacryloyl (GelMA) hydrogels with drug loaded poly(ethylene glycol)- poly(3-caprolactone) (MPEG-PCL) nanoparticles dispersed in the hydrogel matrix, is rapidly fabricated by a continuous three-dimensional (3D) printing process. While the 3D-printed hydrogel conduit with customized size, shape and structure provides a physical microenvironment for axonal elongation, the nanoparticles sustained release the drug to facilitate the nerve regeneration. The drug, 44(5,10-dimethyl-6-oxo-6,10-dihydro-5H-pyrimido[5,4-b]thieno[3,2-e][1,4]diazepin-2-yl)amino) benzenesulfonamide, is a Hippo pathway inhibitor with multiple functions including improving the proliferation and migration of Schwann cells and up-regulating neurotrophic factors genes. The descried functional nerve conduit efficiently induced the recovery of sciatic injuries in morphology, histopathology and functions in vivo, showing the potential clinical application in peripheral nerve repair.
Statements of Significance
Functional nerve conduit provides a promising strategy alternative to autografts. In this work, we rapidly customized a nanoparticle-enhanced conduit by the continuous bioprinting process. This nanoparticle in the conduit can release a Hippo pathway inhibitor to facilitate the nerve regeneration and function restoration. The efficacy of the conduits is comparable to that of autograft, suggesting the potential clinical applications. (C) 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
  author       = {Tao, Jie and Zhang, Jiumeng and Du, Ting and Xu, Xin and Deng, Xianming and Chen, Shaochen and Liu, Jinlu and Chen, Yuwen and Liu, Xuan and Xiong, Meimei and Luo, Yi and Cheng, Hao and Mao, Jian and Cardon, Ludwig and Gou, Maling and Wei, Yuquan},
  issn         = {1742-7061},
  journal      = {ACTA BIOMATERIALIA },
  keywords     = {Biotechnology,Biochemistry,Molecular Biology,Biomaterials,Biomedical Engineering,General Medicine,3D printing,Nanoparticles,Hydrogel,Peripheral nerve regeneration,Drug delivery,HIPPO PATHWAY,TISSUE HOMEOSTASIS,REGENERATION,STRATEGIES,INJURY,YAP,DELIVERY,GUIDANCE,CELLS,GAP},
  language     = {eng},
  pages        = {49--59},
  publisher    = {Elsevier },
  title        = {Rapid 3D printing of functional nanoparticle-enhanced conduits for effective nerve repair},
  url          = {http://dx.doi.org/10.1016/j.actbio.2019.03.047},
  volume       = {90},
  year         = {2019},
}

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