Advanced search
1 file | 804.00 KB

Development and validation of an ultra-high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of iohexol, p-aminohippuric acid and creatinine in porcine and broiler chicken plasma

Laura Dhondt (UGent) , Siska Croubels (UGent) , Pieter De Cock (UGent) , Peter De Paepe (UGent) , Siegrid De Baere (UGent) and Mathias Devreese (UGent)
Author
Organization
Abstract
In order to study the renal function, in terms of glomerular filtration and effective renal plasma flow, in broiler chickens and pigs, an ultra-high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of iohexol, p-aminohippuric acid (PAH) and exogenously administered creatinine in plasma was developed and validated. Sample preparation consisted of a deproteinization step using methanol for porcine plasma and an Ostro (TM) Protein Precipitation & Phospholipid Removal Plate was used for broiler chicken plasma. Chromatographic separation was achieved on a Hypersil Gold aQ column using 0.1% formic acid in water and 0.1% formic acid in methanol as mobile phases. The total run time was limited to 10 min. Matrix-matched calibration curves for iohexol and PAH were prepared and good linearity (r >= 0.9973; gof <= 6.17%) was achieved over the concentration range tested (0.25-90 mu g/mL). Limits of quantification were 0.25 mu g/mL for iohexol and PAH. Water was used as surrogate matrix for analysis of creatinine in plasma. This surrogate calibration curve showed good linearity over the concentration range tested (0.25-90 mu g/mL) (r >= 0.9979; gof <= 5.66%). For creatinine, the relative lower limit of quantification was 201.03 +/- 49.20% and 60.14 +/- 7.64% for chicken and porcine plasma, respectively. The results for within-day and between-day precision and accuracy fell within the specified ranges. This straightforward, cost-effective and rapid method, determining iohexol, PAH and creatinine within one single chromatographic run, has been successfully used for the analysis in porcine and broiler chicken plasma samples in order to determine the renal function of these species.
Keywords
LC-MS/MS, Iohexol, Para-aminohippuric acid, Creatinine, Method development, Chicken plasma, Porcine plasma, GLOMERULAR-FILTRATION-RATE, QUANTITATIVE-DETERMINATION, SERUM CREATININE, EXOGENOUS CREATININE, ENDO-IOHEXOL, HPLC METHOD, CLEARANCE, URINE, FLOW, IOTHALAMATE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 804.00 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Dhondt, Laura, Siska Croubels, Pieter De Cock, Peter De Paepe, Siegrid De Baere, and Mathias Devreese. 2019. “Development and Validation of an Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry Method for the Simultaneous Determination of Iohexol, P-aminohippuric Acid and Creatinine in Porcine and Broiler Chicken Plasma.” Journal of Chromatography B-analytical Technologies in the Biomedical and Life Sciences 1117: 77–85.
APA
Dhondt, L., Croubels, S., De Cock, P., De Paepe, P., De Baere, S., & Devreese, M. (2019). Development and validation of an ultra-high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of iohexol, p-aminohippuric acid and creatinine in porcine and broiler chicken plasma. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1117, 77–85.
Vancouver
1.
Dhondt L, Croubels S, De Cock P, De Paepe P, De Baere S, Devreese M. Development and validation of an ultra-high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of iohexol, p-aminohippuric acid and creatinine in porcine and broiler chicken plasma. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES. 2019;1117:77–85.
MLA
Dhondt, Laura et al. “Development and Validation of an Ultra-high Performance Liquid Chromatography-tandem Mass Spectrometry Method for the Simultaneous Determination of Iohexol, P-aminohippuric Acid and Creatinine in Porcine and Broiler Chicken Plasma.” JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1117 (2019): 77–85. Print.
@article{8616673,
  abstract     = {In order to study the renal function, in terms of glomerular filtration and effective renal plasma flow, in broiler chickens and pigs, an ultra-high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of iohexol, p-aminohippuric acid (PAH) and exogenously administered creatinine in plasma was developed and validated. Sample preparation consisted of a deproteinization step using methanol for porcine plasma and an Ostro (TM) Protein Precipitation & Phospholipid Removal Plate was used for broiler chicken plasma. Chromatographic separation was achieved on a Hypersil Gold aQ column using 0.1% formic acid in water and 0.1% formic acid in methanol as mobile phases. The total run time was limited to 10 min. Matrix-matched calibration curves for iohexol and PAH were prepared and good linearity (r >= 0.9973; gof <= 6.17%) was achieved over the concentration range tested (0.25-90 mu g/mL). Limits of quantification were 0.25 mu g/mL for iohexol and PAH. Water was used as surrogate matrix for analysis of creatinine in plasma. This surrogate calibration curve showed good linearity over the concentration range tested (0.25-90 mu g/mL) (r >= 0.9979; gof <= 5.66%). For creatinine, the relative lower limit of quantification was 201.03 +/- 49.20% and 60.14 +/- 7.64% for chicken and porcine plasma, respectively. The results for within-day and between-day precision and accuracy fell within the specified ranges. 
This straightforward, cost-effective and rapid method, determining iohexol, PAH and creatinine within one single chromatographic run, has been successfully used for the analysis in porcine and broiler chicken plasma samples in order to determine the renal function of these species.},
  author       = {Dhondt, Laura and Croubels, Siska and De Cock, Pieter and De Paepe, Peter and De Baere, Siegrid and Devreese, Mathias},
  issn         = {1570-0232},
  journal      = {JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES},
  keywords     = {LC-MS/MS,Iohexol,Para-aminohippuric acid,Creatinine,Method development,Chicken plasma,Porcine plasma,GLOMERULAR-FILTRATION-RATE,QUANTITATIVE-DETERMINATION,SERUM CREATININE,EXOGENOUS CREATININE,ENDO-IOHEXOL,HPLC METHOD,CLEARANCE,URINE,FLOW,IOTHALAMATE},
  language     = {eng},
  pages        = {77--85},
  title        = {Development and validation of an ultra-high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of iohexol, p-aminohippuric acid and creatinine in porcine and broiler chicken plasma},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2019.04.017},
  volume       = {1117},
  year         = {2019},
}

Altmetric
View in Altmetric
Web of Science
Times cited: