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Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes

Siebe Loontiens (UGent) , Lisa Depestel (UGent) , Suzanne Vanhauwaert (UGent) , Givani Dewyn (UGent) , Charlotte Gistelinck (UGent) , Karen Verboom (UGent) , Wouter Van Loocke (UGent) , Filip Matthijssens (UGent) , Andy Willaert (UGent) , Jo Vandesompele (UGent) , et al.
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Abstract
Background: Transgenic zebrafish lines with the expression of a fluorescent reporter under the control of a cell-type specific promoter, enable transcriptome analysis of FACS sorted cell populations. RNA quality and yield are key determinant factors for accurate expression profiling. Limited cell number and FACS induced cellular stress make RNA isolation of sorted zebrafish cells a delicate process. We aimed to optimize a workflow to extract sufficient amounts of high-quality RNA from a limited number of FACS sorted cells from Tg(fli1a:GFP) zebrafish embryos, which can be used for accurate gene expression analysis. Results: We evaluated two suitable RNA isolation kits (theRNAqueous micro and the RNeasy plus micro kit) and determined that sorting cells directly into lysis buffer is a critical step for success. For low cell numbers, this ensures direct cell lysis, protects RNA from degradation and results in a higher RNA quality and yield. We showed that this works well up to 0.5x dilution of the lysis buffer with sorted cells. In our sort settings, this corresponded to 30,000 and 75,000 cells for the RNAqueous micro kit and RNeasy plus micro kit respectively. Sorting more cells dilutes the lysis buffer too much and requires the use of a collection buffer. We also demonstrated that an additional genomic DNA removal step after RNA isolation is required to completely clear the RNA from any contaminating genomic DNA. For cDNA synthesis and library preparation, we combined SmartSeq v4 full length cDNA library amplification, Nextera XT tagmentation and sample barcoding. Using this workflow, we were able to generate highly reproducible RNA sequencing results. Conclusions: The presented optimized workflow enables to generate high quality RNA and allows accurate transcriptome profiling of small populations of sorted zebrafish cells.
Keywords
RNA isolation, FACS sorting, Zebrafish, RNA sequencing, MODEL, EXPRESSION, GENOME

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Chicago
Loontiens, Siebe, Lisa Depestel, Suzanne Vanhauwaert, Givani Dewyn, Charlotte Gistelinck, Karen Verboom, Wouter Van Loocke, et al. 2019. “Purification of High-quality RNA from a Small Number of Fluorescence Activated Cell Sorted Zebrafish Cells for RNA Sequencing Purposes.” Bmc Genomics 20.
APA
Loontiens, S., Depestel, L., Vanhauwaert, S., Dewyn, G., Gistelinck, C., Verboom, K., Van Loocke, W., et al. (2019). Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes. BMC GENOMICS, 20.
Vancouver
1.
Loontiens S, Depestel L, Vanhauwaert S, Dewyn G, Gistelinck C, Verboom K, et al. Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes. BMC GENOMICS. 2019;20.
MLA
Loontiens, Siebe et al. “Purification of High-quality RNA from a Small Number of Fluorescence Activated Cell Sorted Zebrafish Cells for RNA Sequencing Purposes.” BMC GENOMICS 20 (2019): n. pag. Print.
@article{8616644,
  abstract     = {Background: Transgenic zebrafish lines with the expression of a fluorescent reporter under the control of a cell-type specific promoter, enable transcriptome analysis of FACS sorted cell populations. RNA quality and yield are key determinant factors for accurate expression profiling. Limited cell number and FACS induced cellular stress make RNA isolation of sorted zebrafish cells a delicate process. We aimed to optimize a workflow to extract sufficient amounts of high-quality RNA from a limited number of FACS sorted cells from Tg(fli1a:GFP) zebrafish embryos, which can be used for accurate gene expression analysis.
Results: We evaluated two suitable RNA isolation kits (theRNAqueous micro and the RNeasy plus micro kit) and determined that sorting cells directly into lysis buffer is a critical step for success. For low cell numbers, this ensures direct cell lysis, protects RNA from degradation and results in a higher RNA quality and yield. We showed that this works well up to 0.5x dilution of the lysis buffer with sorted cells. In our sort settings, this corresponded to 30,000 and 75,000 cells for the RNAqueous micro kit and RNeasy plus micro kit respectively. Sorting more cells dilutes the lysis buffer too much and requires the use of a collection buffer. We also demonstrated that an additional genomic DNA removal step after RNA isolation is required to completely clear the RNA from any contaminating genomic DNA. For cDNA synthesis and library preparation, we combined SmartSeq v4 full length cDNA library amplification, Nextera XT tagmentation and sample barcoding. Using this workflow, we were able to generate highly reproducible RNA sequencing results.
Conclusions: The presented optimized workflow enables to generate high quality RNA and allows accurate transcriptome profiling of small populations of sorted zebrafish cells.},
  articleno    = {228},
  author       = {Loontiens, Siebe and Depestel, Lisa and Vanhauwaert, Suzanne and Dewyn, Givani and Gistelinck, Charlotte and Verboom, Karen and Van Loocke, Wouter and Matthijssens, Filip and Willaert, Andy and Vandesompele, Jo and Speleman, Franki and Durinck, Kaat},
  issn         = {1471-2164},
  journal      = {BMC GENOMICS},
  keywords     = {RNA isolation,FACS sorting,Zebrafish,RNA sequencing,MODEL,EXPRESSION,GENOME},
  language     = {eng},
  pages        = {16},
  title        = {Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes},
  url          = {http://dx.doi.org/10.1186/s12864-019-5608-2},
  volume       = {20},
  year         = {2019},
}

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