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Raman and quantitative-phase microscope with counter-propagating beams demonstrated on HeLa cells

Alejandro Diaz Tormo (UGent) , Dmitry Khalenkow (UGent) , Andre Skirtach (UGent) and Nicolas Le Thomas (UGent)
(2019) OSA CONTINUUM. 2(3). p.797-804
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Center for nano- and biophotonics (NB-Photonics)
Abstract
Raman spectroscopy probes the chemical composition of biological samples with sub-micron resolution, rendering it a powerful tool in the diagnosis of several diseases and the study unstained biological samples. However, the weak Raman signal leads to long acquisition times, unsuited to study dynamical processes or large samples. Quantitative phase microscopy can speed up the diagnosis, provide complementary data, and potentially link the Raman fingerprint of the sample with corresponding refractive indices. Here we demonstrate a 4Pi microscope that records both the Raman and quantitative phase information from the same sample spot.

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Citation

Please use this url to cite or link to this publication:

Chicago
Diaz Tormo, Alejandro, Dmitry Khalenkow, Andre Skirtach, and Nicolas Le Thomas. 2019. “Raman and Quantitative-phase Microscope with Counter-propagating Beams Demonstrated on HeLa Cells.” Osa Continuum 2 (3): 797–804.
APA
Diaz Tormo, A., Khalenkow, D., Skirtach, A., & Le Thomas, N. (2019). Raman and quantitative-phase microscope with counter-propagating beams demonstrated on HeLa cells. OSA CONTINUUM, 2(3), 797–804.
Vancouver
1.
Diaz Tormo A, Khalenkow D, Skirtach A, Le Thomas N. Raman and quantitative-phase microscope with counter-propagating beams demonstrated on HeLa cells. OSA CONTINUUM. 2019;2(3):797–804.
MLA
Diaz Tormo, Alejandro et al. “Raman and Quantitative-phase Microscope with Counter-propagating Beams Demonstrated on HeLa Cells.” OSA CONTINUUM 2.3 (2019): 797–804. Print.
@article{8616399,
  abstract     = {Raman spectroscopy probes the chemical composition of biological samples with sub-micron resolution, rendering it a powerful tool in the diagnosis of several diseases and the study unstained biological samples. However, the weak Raman signal leads to long acquisition times, unsuited to study dynamical processes or large samples. Quantitative phase microscopy can speed up the diagnosis, provide complementary data, and potentially link the Raman fingerprint of the sample with corresponding refractive indices. Here we demonstrate a 4Pi microscope that records both the Raman and quantitative phase information from the same sample spot.},
  author       = {Diaz Tormo, Alejandro and Khalenkow, Dmitry and Skirtach, Andre and Le Thomas, Nicolas},
  issn         = {2578-7519},
  journal      = {OSA CONTINUUM},
  language     = {eng},
  number       = {3},
  pages        = {797--804},
  title        = {Raman and quantitative-phase microscope with counter-propagating beams demonstrated on HeLa cells},
  url          = {http://dx.doi.org/10.1364/osac.2.000797},
  volume       = {2},
  year         = {2019},
}

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