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Enrichment and isolation of hydrogen-oxidizing microbiomes

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Abstract
Knowledge about the enrichment of microbial communities growing on hydrogen, oxygen and carbon dioxide is scarce despite numerous studies on axenic cultures of hydrogen-oxidizing bacteria. In the present work, soil was used as an initial mixed culture for the enrichment of autotrophic hydrogen-oxidizing microbiome by means of a continuous supply of hydrogen, oxygen and carbon dioxide during 120 days with 5 media exchanges (5 transfers). The success of the enrichment was evaluated by monitoring ammonium consumption and biomass concentration during 5 transfers. The microbial community showed a clear distinction between the soil and transfers with a presence of numerous heterotrophs at final transfers in spite of hydrogen-oxidizing bacteria enrichment conditions using 16S rRNA amplicon sequencing. Moreover, isolation of some abundant autotrophs and heterotrophs was accomplished under heterotrophic and autotrophic conditions. Hydrogenophaga electricum was isolated and found to be one of the abundant species in most transfers. The findings obtained in this study clearly show a successful workflow to monitor the enrichment for hydrogen-oxidizing bacteria over a short period and retrieving some autotrophic and heterotrophic bacteria from the mixed community.

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MLA
Ehsani, Elham. “Enrichment and Isolation of Hydrogen-oxidizing Microbiomes.” Belgian Society for Microbiology, Symposium Abstracts. 2018. Print.
APA
Ehsani, E. (2018). Enrichment and isolation of hydrogen-oxidizing microbiomes. Belgian Society for Microbiology, Symposium abstracts. Presented at the 2018 Symposium of the Belgian Society for Microbiology: Microbes in the spotlight.
Chicago author-date
Ehsani, Elham. 2018. “Enrichment and Isolation of Hydrogen-oxidizing Microbiomes.” In Belgian Society for Microbiology, Symposium Abstracts.
Chicago author-date (all authors)
Ehsani, Elham. 2018. “Enrichment and Isolation of Hydrogen-oxidizing Microbiomes.” In Belgian Society for Microbiology, Symposium Abstracts.
Vancouver
1.
Ehsani E. Enrichment and isolation of hydrogen-oxidizing microbiomes. Belgian Society for Microbiology, Symposium abstracts. 2018.
IEEE
[1]
E. Ehsani, “Enrichment and isolation of hydrogen-oxidizing microbiomes,” in Belgian Society for Microbiology, Symposium abstracts, Brussels, Belgium, 2018.
@inproceedings{8615502,
  abstract     = {{Knowledge about the enrichment of microbial communities growing on hydrogen, oxygen and carbon dioxide is scarce despite numerous studies on axenic cultures of hydrogen-oxidizing bacteria. In the present work, soil was used as an initial mixed culture for the enrichment of autotrophic hydrogen-oxidizing microbiome by means of a continuous supply of hydrogen, oxygen and carbon dioxide during 120 days with 5 media exchanges (5 transfers). The success of the enrichment was evaluated by monitoring ammonium consumption and biomass concentration during 5 transfers. The microbial community showed a clear distinction between the soil and transfers with a presence of numerous heterotrophs at final transfers in spite of hydrogen-oxidizing bacteria enrichment conditions using 16S rRNA amplicon sequencing. Moreover, isolation of some abundant autotrophs and heterotrophs was accomplished under heterotrophic and autotrophic conditions. Hydrogenophaga electricum was isolated and found to be one of the abundant species in most transfers. The findings obtained in this study clearly show a successful workflow to monitor the enrichment for hydrogen-oxidizing bacteria over a short period and retrieving some autotrophic and heterotrophic bacteria from the mixed community.}},
  author       = {{Ehsani, Elham}},
  booktitle    = {{Belgian Society for Microbiology, Symposium abstracts}},
  language     = {{eng}},
  location     = {{Brussels, Belgium}},
  title        = {{Enrichment and isolation of hydrogen-oxidizing microbiomes}},
  year         = {{2018}},
}