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Establishment of a rat model for pressurized intraperitoneal aerosol chemotherapy

Leen Van de Sande (UGent) , Wouter Willaert (UGent) , Sarah Cosyns (UGent) and Wim Ceelen (UGent)
(2017) EUROPEAN SURGICAL RESEARCH. 58(suppl. 2).
Author
Organization
Abstract
Background: Peritoneal carcinomatosis is an unmet medical need. Intraperitoneal chemotherapy has limited drug distribution and poor peritoneal penetration. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) has shown to improve drug uptake by utilizing the physical properties of gas and pressure. In this study, a rat model was developed to further enhance the pharmacologic properties of PIPAC. Methods: Preliminary pilot studies were performed to determine the most suitable parameters in our animal model. Optimal flow rate, injection volume, injection pressure and pressure of pneumoperitoneum were determined to obtain a homogeneous aerosol. Subsequently, a rat model for PIPAC was established with these parameters. To simulate a PIPAC procedure, we used 5 mm balloon trocars, a nebulizer, a high pressure injector, a laparoscope and a closed aerosol waste system. Results: It has been found from the preliminary pilot studies that the most suitable parameters are the following: nebulization of 8 ml 0.9% NaCl, a flow rate of 0.5 ml/s, an injection pressure of 20 bar and a pneumoperitoneum of 4 mm Hg. Subsequently, PIPAC was performed in the rat model (n = 3). All rats survived and there was no leakage observed over the entire setup. Conclusions: The PIPAC procedure was found to be feasible and safe in a rat model. In the future, we aim to study 1) the pharmacological and physical properties of the aerosol in the rat model and 2) the efficacy of several cytostatics into a SKOV3 xenograft rat model. This way, an attempt will be made to improve the treatment of peritoneal carcinomatosis.

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MLA
Van de Sande, Leen et al. “Establishment of a Rat Model for Pressurized Intraperitoneal Aerosol Chemotherapy.” European Surgical Research. Vol. 58. 2017. Print.
APA
Van de Sande, L., Willaert, W., Cosyns, S., & Ceelen, W. (2017). Establishment of a rat model for pressurized intraperitoneal aerosol chemotherapy. EUROPEAN SURGICAL RESEARCH (Vol. 58). Presented at the 52nd Congress of the European Society for Surgical Research.
Chicago author-date
Van de Sande, Leen, Wouter Willaert, Sarah Cosyns, and Wim Ceelen. 2017. “Establishment of a Rat Model for Pressurized Intraperitoneal Aerosol Chemotherapy.” In European Surgical Research. Vol. 58.
Chicago author-date (all authors)
Van de Sande, Leen, Wouter Willaert, Sarah Cosyns, and Wim Ceelen. 2017. “Establishment of a Rat Model for Pressurized Intraperitoneal Aerosol Chemotherapy.” In European Surgical Research. Vol. 58.
Vancouver
1.
Van de Sande L, Willaert W, Cosyns S, Ceelen W. Establishment of a rat model for pressurized intraperitoneal aerosol chemotherapy. EUROPEAN SURGICAL RESEARCH. 2017.
IEEE
[1]
L. Van de Sande, W. Willaert, S. Cosyns, and W. Ceelen, “Establishment of a rat model for pressurized intraperitoneal aerosol chemotherapy,” in EUROPEAN SURGICAL RESEARCH, Amsterdam, The Netherlands, 2017, vol. 58, no. suppl. 2.
@inproceedings{8615397,
  abstract     = {Background: Peritoneal carcinomatosis is an unmet medical need. Intraperitoneal chemotherapy has limited drug distribution and poor peritoneal penetration. Pressurized intraperitoneal aerosol chemotherapy (PIPAC) has shown to improve drug uptake by utilizing the physical properties of gas and pressure. In this study, a rat model was developed to further enhance the pharmacologic properties of PIPAC.
Methods: Preliminary pilot studies were performed to determine the most suitable parameters in our animal model. Optimal flow rate, injection volume, injection pressure and pressure of pneumoperitoneum were determined to obtain a homogeneous aerosol. Subsequently, a rat model for PIPAC was established with these parameters. To simulate a PIPAC procedure, we used 5 mm balloon trocars, a nebulizer, a high pressure injector, a laparoscope and a closed aerosol waste system.
Results: It has been found from the preliminary pilot studies that the most suitable parameters are the following: nebulization of 8 ml 0.9% NaCl, a flow rate of 0.5 ml/s, an injection pressure of 20 bar and a pneumoperitoneum of 4 mm Hg. Subsequently, PIPAC was performed in the rat model (n = 3). All rats survived and there was no leakage observed over the entire setup.
Conclusions: The PIPAC procedure was found to be feasible and safe in a rat model. In the future, we aim to study 1) the pharmacological and physical properties of the aerosol in the rat model and 2) the efficacy of several cytostatics into a SKOV3 xenograft rat model. This way, an attempt will be made to improve the treatment of peritoneal carcinomatosis.},
  articleno    = {abstract 32},
  author       = {Van de Sande, Leen and Willaert, Wouter and Cosyns, Sarah and Ceelen, Wim},
  booktitle    = {EUROPEAN SURGICAL RESEARCH},
  issn         = {0014-312X},
  language     = {eng},
  location     = {Amsterdam, The Netherlands},
  number       = {suppl. 2},
  title        = {Establishment of a rat model for pressurized intraperitoneal aerosol chemotherapy},
  volume       = {58},
  year         = {2017},
}