Advanced search
1 file | 1.81 MB Add to list

Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells

Author
Organization
Abstract
In contrast to mouse, human female germ cells develop asynchronously. Germ cells transition to meiosis, erase genomic imprints, and reactivate the X chromosome. It is unknown if these events all appear asynchronously, and how they relate to each other. Here we combine exome sequencing of human fetal and maternal tissues with single-cell RNA-sequencing of five donors. We reconstruct full parental haplotypes and quantify changes in parental allele-specific expression, genome-wide. First we distinguish primordial germ cells (PGC), pre-meiotic, and meiotic transcriptional stages. Next we demonstrate that germ cells from various stages monoallelically express imprinted genes and confirm this by methylation patterns. Finally, we show that roughly 30% of the PGCs are still reactivating their inactive X chromosome and that this is related to transcriptional stage rather than fetal age. Altogether, we uncover the complexity and cell-to-cell heterogeneity of transcriptional and epigenetic remodeling in female human germ cells.
Keywords
X-CHROMOSOME INACTIVATION, EMBRYONIC STEM-CELLS, LONG NONCODING RNAS, DNA-SEQUENCING DATA, GENOME, GENES, FATE, FRAMEWORK, DYNAMICS, DOMAIN

Downloads

  • s41467-018-04215-7.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.81 MB

Citation

Please use this url to cite or link to this publication:

MLA
Vertesy, Abel et al. “Parental Haplotype-specific Single-cell Transcriptomics Reveal Incomplete Epigenetic Reprogramming in Human Female Germ Cells.” NATURE COMMUNICATIONS 9 (2018): n. pag. Print.
APA
Vertesy, A., Arindrarto, W., Roost, M. S., Reinius, B., Torrens-Juaneda, V., Bialecka, M., Moustakas, I., et al. (2018). Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells. NATURE COMMUNICATIONS, 9.
Chicago author-date
Vertesy, Abel, Wibowo Arindrarto, Matthias S Roost, Bjorn Reinius, Vanessa Torrens-Juaneda, Monika Bialecka, Ioannis Moustakas, et al. 2018. “Parental Haplotype-specific Single-cell Transcriptomics Reveal Incomplete Epigenetic Reprogramming in Human Female Germ Cells.” Nature Communications 9.
Chicago author-date (all authors)
Vertesy, Abel, Wibowo Arindrarto, Matthias S Roost, Bjorn Reinius, Vanessa Torrens-Juaneda, Monika Bialecka, Ioannis Moustakas, Yavuz Ariyurek, Ewart Kuijk, Hailiang Mei, Rickard Sandberg, Alexander van Oudenaarden, and Susana Marina Chuva de Sousa Lopes. 2018. “Parental Haplotype-specific Single-cell Transcriptomics Reveal Incomplete Epigenetic Reprogramming in Human Female Germ Cells.” Nature Communications 9.
Vancouver
1.
Vertesy A, Arindrarto W, Roost MS, Reinius B, Torrens-Juaneda V, Bialecka M, et al. Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells. NATURE COMMUNICATIONS. 2018;9.
IEEE
[1]
A. Vertesy et al., “Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells,” NATURE COMMUNICATIONS, vol. 9, 2018.
@article{8615353,
  abstract     = {In contrast to mouse, human female germ cells develop asynchronously. Germ cells transition to meiosis, erase genomic imprints, and reactivate the X chromosome. It is unknown if these events all appear asynchronously, and how they relate to each other. Here we combine exome sequencing of human fetal and maternal tissues with single-cell RNA-sequencing of five donors. We reconstruct full parental haplotypes and quantify changes in parental allele-specific expression, genome-wide. First we distinguish primordial germ cells (PGC), pre-meiotic, and meiotic transcriptional stages. Next we demonstrate that germ cells from various stages monoallelically express imprinted genes and confirm this by methylation patterns. Finally, we show that roughly 30% of the PGCs are still reactivating their inactive X chromosome and that this is related to transcriptional stage rather than fetal age. Altogether, we uncover the complexity and cell-to-cell heterogeneity of transcriptional and epigenetic remodeling in female human germ cells.},
  articleno    = {1873},
  author       = {Vertesy, Abel and Arindrarto, Wibowo and Roost, Matthias S and Reinius, Bjorn and Torrens-Juaneda, Vanessa and Bialecka, Monika and Moustakas, Ioannis and Ariyurek, Yavuz and Kuijk, Ewart and Mei, Hailiang and Sandberg, Rickard and van Oudenaarden, Alexander and Chuva de Sousa Lopes, Susana Marina},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  keywords     = {X-CHROMOSOME INACTIVATION,EMBRYONIC STEM-CELLS,LONG NONCODING RNAS,DNA-SEQUENCING DATA,GENOME,GENES,FATE,FRAMEWORK,DYNAMICS,DOMAIN},
  language     = {eng},
  pages        = {10},
  title        = {Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells},
  url          = {http://dx.doi.org/10.1038/s41467-018-04215-7},
  volume       = {9},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: