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3D modeling of esophageal development using human PSC-derived basal progenitors reveals a critical role for notch signaling

(2018) CELL STEM CELL. 23(4). p.516-529
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Abstract
Pluripotent stem cells (PSCs) could provide a powerful system to model development of the human esophagus, whose distinct tissue organization compared to rodent esophagus suggests that developmental mechanisms may not be conserved between species. We therefore established an efficient protocol for generating esophageal progenitor cells (EPCs) from human PSCs. We found that inhibition of TGF beta and BMP signaling is required for sequential specification of EPCs, which can be further purified using cell-surface markers. These EPCs resemble their human fetal counterparts and can recapitulate normal development of esophageal stratified squamous epithelium during in vitro 3D cultures and in vivo. Importantly, combining hPSC differentiation strategies with mouse genetics elucidated a critical role for Notch signaling in the formation of this epithelium. These studies therefore not only provide an efficient approach to generate EPCs, but also offer a model system to study the regulatory mechanisms underlying development of the human esophagus.
Keywords
PLURIPOTENT STEM-CELLS, FOREGUT ENDODERM, EFFICIENT DERIVATION, LUNG, PROGENITORS, ANTERIOR FOREGUT, EPITHELIAL-CELLS, MOUSE ESOPHAGUS, INNER-EAR, DIFFERENTIATION, GENE

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MLA
Zhang, Yongchun et al. “3D Modeling of Esophageal Development Using Human PSC-derived Basal Progenitors Reveals a Critical Role for Notch Signaling.” CELL STEM CELL 23.4 (2018): 516–529. Print.
APA
Zhang, Yongchun, Yang, Y., Jiang, M., Huang, S. X., Zhang, W., Al Alam, D., Danopoulos, S., et al. (2018). 3D modeling of esophageal development using human PSC-derived basal progenitors reveals a critical role for notch signaling. CELL STEM CELL, 23(4), 516–529.
Chicago author-date
Zhang, Yongchun, Ying Yang, Ming Jiang, Sarah Xuelian Huang, Wanwei Zhang, Denise Al Alam, Soula Danopoulos, et al. 2018. “3D Modeling of Esophageal Development Using Human PSC-derived Basal Progenitors Reveals a Critical Role for Notch Signaling.” Cell Stem Cell 23 (4): 516–529.
Chicago author-date (all authors)
Zhang, Yongchun, Ying Yang, Ming Jiang, Sarah Xuelian Huang, Wanwei Zhang, Denise Al Alam, Soula Danopoulos, Munemasa Mori, Ya-Wen Chen, Revathi Balasubramanian, Susana Marina Chuva de Sousa Lopes, Carlos Serra, Monika Bialecka, Eugene Kim, Sijie Lin, Ana Luisa Rodrigues Toste de Carvalho, Paul N Riccio, Wellington V Cardoso, Xin Zhang, Hans-Willem Snoeck, and Jianwen Que. 2018. “3D Modeling of Esophageal Development Using Human PSC-derived Basal Progenitors Reveals a Critical Role for Notch Signaling.” Cell Stem Cell 23 (4): 516–529.
Vancouver
1.
Zhang Y, Yang Y, Jiang M, Huang SX, Zhang W, Al Alam D, et al. 3D modeling of esophageal development using human PSC-derived basal progenitors reveals a critical role for notch signaling. CELL STEM CELL. 2018;23(4):516–29.
IEEE
[1]
Y. Zhang et al., “3D modeling of esophageal development using human PSC-derived basal progenitors reveals a critical role for notch signaling,” CELL STEM CELL, vol. 23, no. 4, pp. 516–529, 2018.
@article{8615348,
  abstract     = {Pluripotent stem cells (PSCs) could provide a powerful system to model development of the human esophagus, whose distinct tissue organization compared to rodent esophagus suggests that developmental mechanisms may not be conserved between species. We therefore established an efficient protocol for generating esophageal progenitor cells (EPCs) from human PSCs. We found that inhibition of TGF beta and BMP signaling is required for sequential specification of EPCs, which can be further purified using cell-surface markers. These EPCs resemble their human fetal counterparts and can recapitulate normal development of esophageal stratified squamous epithelium during in vitro 3D cultures and in vivo. Importantly, combining hPSC differentiation strategies with mouse genetics elucidated a critical role for Notch signaling in the formation of this epithelium. These studies therefore not only provide an efficient approach to generate EPCs, but also offer a model system to study the regulatory mechanisms underlying development of the human esophagus.},
  author       = {Zhang, Yongchun and Yang, Ying and Jiang, Ming and Huang, Sarah Xuelian and Zhang, Wanwei and Al Alam, Denise and Danopoulos, Soula and Mori, Munemasa and Chen, Ya-Wen and Balasubramanian, Revathi and Chuva de Sousa Lopes, Susana Marina and Serra, Carlos and Bialecka, Monika and Kim, Eugene and Lin, Sijie and Rodrigues Toste de Carvalho, Ana Luisa and Riccio, Paul N and Cardoso, Wellington V and Zhang, Xin and Snoeck, Hans-Willem and Que, Jianwen},
  issn         = {1934-5909},
  journal      = {CELL STEM CELL},
  keywords     = {PLURIPOTENT STEM-CELLS,FOREGUT ENDODERM,EFFICIENT DERIVATION,LUNG,PROGENITORS,ANTERIOR FOREGUT,EPITHELIAL-CELLS,MOUSE ESOPHAGUS,INNER-EAR,DIFFERENTIATION,GENE},
  language     = {eng},
  number       = {4},
  pages        = {516--529},
  title        = {3D modeling of esophageal development using human PSC-derived basal progenitors reveals a critical role for notch signaling},
  url          = {http://dx.doi.org/10.1016/j.stem.2018.08.009},
  volume       = {23},
  year         = {2018},
}

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