Analytical approach for detection of ergosterol in mushrooms based on modification free electrochemical sensor in organic solvents
- Author
- Vesna Vukojevic, Sladjana Djurdjic, Lubomir Svorc, Tanja Cirkovic Velickovic (UGent) , Jelena Mutic and Dalibor M Stankovic
- Organization
- Abstract
- Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.
- Keywords
- BORON-DOPED DIAMOND, CHROMATOGRAPHY-MASS SPECTROMETRY, VOLTAMMETRIC, DETERMINATION, URINE SAMPLES, ELECTRODE, EXTRACTION, BEHAVIOR, CHOLESTEROL, DRUG, PHARMACEUTICALS, Ergosterol, Boron-doped diamond electrode, Mushrooms
Downloads
-
(...).pdf
- full text (Published version)
- |
- UGent only
- |
- |
- 1.30 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8615090
- MLA
- Vukojevic, Vesna, et al. “Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents.” FOOD ANALYTICAL METHODS, vol. 11, no. 9, 2018, pp. 2590–96, doi:10.1007/s12161-018-1249-3.
- APA
- Vukojevic, V., Djurdjic, S., Svorc, L., Cirkovic Velickovic, T., Mutic, J., & Stankovic, D. M. (2018). Analytical approach for detection of ergosterol in mushrooms based on modification free electrochemical sensor in organic solvents. FOOD ANALYTICAL METHODS, 11(9), 2590–2596. https://doi.org/10.1007/s12161-018-1249-3
- Chicago author-date
- Vukojevic, Vesna, Sladjana Djurdjic, Lubomir Svorc, Tanja Cirkovic Velickovic, Jelena Mutic, and Dalibor M Stankovic. 2018. “Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents.” FOOD ANALYTICAL METHODS 11 (9): 2590–96. https://doi.org/10.1007/s12161-018-1249-3.
- Chicago author-date (all authors)
- Vukojevic, Vesna, Sladjana Djurdjic, Lubomir Svorc, Tanja Cirkovic Velickovic, Jelena Mutic, and Dalibor M Stankovic. 2018. “Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents.” FOOD ANALYTICAL METHODS 11 (9): 2590–2596. doi:10.1007/s12161-018-1249-3.
- Vancouver
- 1.Vukojevic V, Djurdjic S, Svorc L, Cirkovic Velickovic T, Mutic J, Stankovic DM. Analytical approach for detection of ergosterol in mushrooms based on modification free electrochemical sensor in organic solvents. FOOD ANALYTICAL METHODS. 2018;11(9):2590–6.
- IEEE
- [1]V. Vukojevic, S. Djurdjic, L. Svorc, T. Cirkovic Velickovic, J. Mutic, and D. M. Stankovic, “Analytical approach for detection of ergosterol in mushrooms based on modification free electrochemical sensor in organic solvents,” FOOD ANALYTICAL METHODS, vol. 11, no. 9, pp. 2590–2596, 2018.
@article{8615090,
abstract = {{Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag+ non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 mu M was estimated, with limit of detection of 0.7 mu M, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.}},
author = {{Vukojevic, Vesna and Djurdjic, Sladjana and Svorc, Lubomir and Cirkovic Velickovic, Tanja and Mutic, Jelena and Stankovic, Dalibor M}},
issn = {{1936-9751}},
journal = {{FOOD ANALYTICAL METHODS}},
keywords = {{BORON-DOPED DIAMOND,CHROMATOGRAPHY-MASS SPECTROMETRY,VOLTAMMETRIC,DETERMINATION,URINE SAMPLES,ELECTRODE,EXTRACTION,BEHAVIOR,CHOLESTEROL,DRUG,PHARMACEUTICALS,Ergosterol,Boron-doped diamond electrode,Mushrooms}},
language = {{eng}},
number = {{9}},
pages = {{2590--2596}},
title = {{Analytical approach for detection of ergosterol in mushrooms based on modification free electrochemical sensor in organic solvents}},
url = {{http://doi.org/10.1007/s12161-018-1249-3}},
volume = {{11}},
year = {{2018}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: