Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 869.10 KB
Add to list
  1. Search results
  2. Specific uptake of tumor necrosis fac...

Specific uptake of tumor necrosis factor-α is involved in growth control of Trypanosoma brucei

Stefan Magez (UGent) , M Geuskens, A Beschin, H del Favero, H Verschueren, R Lucas, E Pays and P de Baetselier
(1997) JOURNAL OF CELL BIOLOGY. 137(3). p.715-727
Author
Organization
Abstract
Trypanosoma brucei is lysed by tumor necrosis factor-alpha (TNF-alpha) in a dose-dependent way, involving specific binding of the cytokine to a trypanosomal glycoprotein present in the flagellar pocket of the parasite. TNF-alpha-gold particles are endocytosed via coated pits and vesicles and are directed towards lysosome-like digestive organelles. The specific uptake of the cytokine by the parasite results in a developmentally regulated loss of osmoregulatory capacity. TNF-alpha specific lysis is prevented when lysis assays are performed at a temperature <26 degrees C, despite uptake of the cytokine. Inhibition of lysis is also observed when a lysosomotropic agent is added during the first 2 h of incubation. Both monomorphic and pleomorphic trypanosomes are lysed but only when isolated during the peak of parasitaemia. Lysis is not observed with early infection stage parasites or procyclic (insect-specific) forms. Anti-TNF-alpha treatment of T. brucei-infected mice reveals a dramatic increase in parasitaemia in the blood circulation, the spleen, the lymph nodes, and the peritoneal cavity. These data suggest that in the mammalian host, TNF-alpha is involved in the growth control of T. brucei.
Keywords
AFRICAN TRYPANOSOMES, BLOOD-STREAM, PROTECTIVE ROLE, FACTOR PLAYS, TNF-ALPHA, SURFACE, RECEPTOR, BINDING, EXPRESSION, MICE

Downloads

  • Download
    (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 869.10 KB

Citation

Please use this url to cite or link to this publication:

MLA
Magez, Stefan, et al. “Specific Uptake of Tumor Necrosis Factor-α Is Involved in Growth Control of Trypanosoma Brucei.” JOURNAL OF CELL BIOLOGY, vol. 137, no. 3, 1997, pp. 715–27, doi:10.1083/jcb.137.3.715.
APA
Magez, S., Geuskens, M., Beschin, A., del Favero, H., Verschueren, H., Lucas, R., … de Baetselier, P. (1997). Specific uptake of tumor necrosis factor-α is involved in growth control of Trypanosoma brucei. JOURNAL OF CELL BIOLOGY, 137(3), 715–727. https://doi.org/10.1083/jcb.137.3.715
Chicago author-date
Magez, Stefan, M Geuskens, A Beschin, H del Favero, H Verschueren, R Lucas, E Pays, and P de Baetselier. 1997. “Specific Uptake of Tumor Necrosis Factor-α Is Involved in Growth Control of Trypanosoma Brucei.” JOURNAL OF CELL BIOLOGY 137 (3): 715–27. https://doi.org/10.1083/jcb.137.3.715.
Chicago author-date (all authors)
Magez, Stefan, M Geuskens, A Beschin, H del Favero, H Verschueren, R Lucas, E Pays, and P de Baetselier. 1997. “Specific Uptake of Tumor Necrosis Factor-α Is Involved in Growth Control of Trypanosoma Brucei.” JOURNAL OF CELL BIOLOGY 137 (3): 715–727. doi:10.1083/jcb.137.3.715.
Vancouver
1.
Magez S, Geuskens M, Beschin A, del Favero H, Verschueren H, Lucas R, et al. Specific uptake of tumor necrosis factor-α is involved in growth control of Trypanosoma brucei. JOURNAL OF CELL BIOLOGY. 1997;137(3):715–27.
IEEE
[1]
S. Magez et al., “Specific uptake of tumor necrosis factor-α is involved in growth control of Trypanosoma brucei,” JOURNAL OF CELL BIOLOGY, vol. 137, no. 3, pp. 715–727, 1997.
@article{8615034,
  abstract     = {{Trypanosoma brucei is lysed by tumor necrosis factor-alpha (TNF-alpha) in a dose-dependent way, involving specific binding of the cytokine to a trypanosomal glycoprotein present in the flagellar pocket of the parasite. TNF-alpha-gold particles are endocytosed via coated pits and vesicles and are directed towards lysosome-like digestive organelles. The specific uptake of the cytokine by the parasite results in a developmentally regulated loss of osmoregulatory capacity. TNF-alpha specific lysis is prevented when lysis assays are performed at a temperature <26 degrees C, despite uptake of the cytokine. Inhibition of lysis is also observed when a lysosomotropic agent is added during the first 2 h of incubation. Both monomorphic and pleomorphic trypanosomes are lysed but only when isolated during the peak of parasitaemia. Lysis is not observed with early infection stage parasites or procyclic (insect-specific) forms. Anti-TNF-alpha treatment of T. brucei-infected mice reveals a dramatic increase in parasitaemia in the blood circulation, the spleen, the lymph nodes, and the peritoneal cavity. These data suggest that in the mammalian host, TNF-alpha is involved in the growth control of T. brucei.}},
  author       = {{Magez, Stefan and Geuskens, M and Beschin, A and del Favero, H and Verschueren, H and Lucas, R and Pays, E and de Baetselier, P}},
  issn         = {{0021-9525}},
  journal      = {{JOURNAL OF CELL BIOLOGY}},
  keywords     = {{AFRICAN TRYPANOSOMES,BLOOD-STREAM,PROTECTIVE ROLE,FACTOR PLAYS,TNF-ALPHA,SURFACE,RECEPTOR,BINDING,EXPRESSION,MICE}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{715--727}},
  title        = {{Specific uptake of tumor necrosis factor-α is involved in growth control of Trypanosoma brucei}},
  url          = {{http://doi.org/10.1083/jcb.137.3.715}},
  volume       = {{137}},
  year         = {{1997}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: