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Granular fermentation enables high rate caproic acid production from solid-free thin stillage

(2019) GREEN CHEMISTRY. 21(6). p.1330-1339
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Abstract
Microbial chain elongation has been investigated as a process to upgrade diluted ethanol to more valuable medium chain carboxylic acids. However, its application in the valorization of (agro)industrial wastes or sidestreams has been hampered by low volumetric production rates. Microbial granulation, widely applied in wastewater treatment, has been rarely explored in a context of bioproduction. Here we show how fermentative granulation can be applied to the valorization of solid-free thin stillage, through a high-rate chain elongation process for the production of MCCA without the addition of exogenous electron donating intermediates. Using a fermentative expanded granular sludge bed reactor, caproic acid was produced at sustained rates of 12.3 g C6 L-1 d(-1) (625 mmol C L-1 d(-1) or 27.2 g C6-COD L-1 d(-1)) at pH 5.5, and concentrations of up to 6.8 g C6 L-1. During a stable period (days 181-217) the 27.2% of the influent fermentable COD was converted to caproic acid. During the same period caproic acid accounted for 44.4% of the produced carboxylic acid-C, with butyric and acetic acids generated as main side-products, but a maximum specificity of 58.4% had been previously observed. Lactic acid served as the electron donating intermediate, to the extent that no net ethanol was consumed. The granules developed into disc-shaped aggregates and were enriched in chain elongating Ruminococaceae, while the planktonic biomass was dominated by lactic acid bacteria. The characterization of the extracellular polymeric substances (EPS) showed that polymers of high molecular weight (>10(6) Da) were more abundant in the granules than in the planktonic biomass.
Keywords
UPGRADING DILUTE ETHANOL, CHAIN CARBOXYLIC-ACIDS, WASTE-WATER-TREATMENT, N-CAPROATE, GEN. NOV., REACTOR, ELONGATION, LACTATE, CONVERSION, GALACTITOL

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Citation

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MLA
Carvajal Arroyo, Jose Maria et al. “Granular Fermentation Enables High Rate Caproic Acid Production from Solid-free Thin Stillage.” GREEN CHEMISTRY 21.6 (2019): 1330–1339. Print.
APA
Carvajal Arroyo, J. M., Candry, P., Andersen, S. J., Props, R., Seviour, T., Ganigué, R., & Rabaey, K. (2019). Granular fermentation enables high rate caproic acid production from solid-free thin stillage. GREEN CHEMISTRY, 21(6), 1330–1339.
Chicago author-date
Carvajal Arroyo, Jose Maria, Pieter Candry, Stephen J Andersen, Ruben Props, Thomas Seviour, Ramon Ganigué, and Korneel Rabaey. 2019. “Granular Fermentation Enables High Rate Caproic Acid Production from Solid-free Thin Stillage.” Green Chemistry 21 (6): 1330–1339.
Chicago author-date (all authors)
Carvajal Arroyo, Jose Maria, Pieter Candry, Stephen J Andersen, Ruben Props, Thomas Seviour, Ramon Ganigué, and Korneel Rabaey. 2019. “Granular Fermentation Enables High Rate Caproic Acid Production from Solid-free Thin Stillage.” Green Chemistry 21 (6): 1330–1339.
Vancouver
1.
Carvajal Arroyo JM, Candry P, Andersen SJ, Props R, Seviour T, Ganigué R, et al. Granular fermentation enables high rate caproic acid production from solid-free thin stillage. GREEN CHEMISTRY. 2019;21(6):1330–9.
IEEE
[1]
J. M. Carvajal Arroyo et al., “Granular fermentation enables high rate caproic acid production from solid-free thin stillage,” GREEN CHEMISTRY, vol. 21, no. 6, pp. 1330–1339, 2019.
@article{8614845,
  abstract     = {Microbial chain elongation has been investigated as a process to upgrade diluted ethanol to more valuable medium chain carboxylic acids. However, its application in the valorization of (agro)industrial wastes or sidestreams has been hampered by low volumetric production rates. Microbial granulation, widely applied in wastewater treatment, has been rarely explored in a context of bioproduction. Here we show how fermentative granulation can be applied to the valorization of solid-free thin stillage, through a high-rate chain elongation process for the production of MCCA without the addition of exogenous electron donating intermediates. Using a fermentative expanded granular sludge bed reactor, caproic acid was produced at sustained rates of 12.3 g C6 L-1 d(-1) (625 mmol C L-1 d(-1) or 27.2 g C6-COD L-1 d(-1)) at pH 5.5, and concentrations of up to 6.8 g C6 L-1. During a stable period (days 181-217) the 27.2% of the influent fermentable COD was converted to caproic acid. During the same period caproic acid accounted for 44.4% of the produced carboxylic acid-C, with butyric and acetic acids generated as main side-products, but a maximum specificity of 58.4% had been previously observed. Lactic acid served as the electron donating intermediate, to the extent that no net ethanol was consumed. The granules developed into disc-shaped aggregates and were enriched in chain elongating Ruminococaceae, while the planktonic biomass was dominated by lactic acid bacteria. The characterization of the extracellular polymeric substances (EPS) showed that polymers of high molecular weight (>10(6) Da) were more abundant in the granules than in the planktonic biomass.},
  author       = {Carvajal Arroyo, Jose Maria and Candry, Pieter and Andersen, Stephen J and Props, Ruben and Seviour, Thomas and Ganigué, Ramon and Rabaey, Korneel},
  issn         = {1463-9262},
  journal      = {GREEN CHEMISTRY},
  keywords     = {UPGRADING DILUTE ETHANOL,CHAIN CARBOXYLIC-ACIDS,WASTE-WATER-TREATMENT,N-CAPROATE,GEN. NOV.,REACTOR,ELONGATION,LACTATE,CONVERSION,GALACTITOL},
  language     = {eng},
  number       = {6},
  pages        = {1330--1339},
  title        = {Granular fermentation enables high rate caproic acid production from solid-free thin stillage},
  url          = {http://dx.doi.org/10.1039/c8gc03648a},
  volume       = {21},
  year         = {2019},
}

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