Advanced search
2 files | 2.90 MB

High-resolution QTL mapping in Tetranychus urticae reveals acaricide-specific responses and common target-site resistance after selection by different METI-I acaricides

Author
Organization
Project
POLYADAPT (Molecular-genetic mechanisms of extreme adaptation in a polyphagous agricultural pest)
Project
SuperPests (Innovative tools for rational control of the most difficult-to-manage pests (super pests) and the diseases they transmit)
Abstract
Arthropod herbivores cause dramatic crop losses, and frequent pesticide use has led to widespread resistance in numerous species. One such species, the two-spotted spider mite, Tetranychus urticae, is an extreme generalist herbivore and a major worldwide crop pest with a history of rapidly developing resistance to acaricides. Mitochondrial Electron Transport Inhibitors of complex I (METI-Is) have been used extensively in the last 25 years to control T. urticae around the globe, and widespread resistance to each has been documented. METI-I resistance mechanisms in T. urticae are likely complex, as increased metabolism by cytochrome P450 monooxygenases as well as a target-site mutation have been linked with resistance. To identify loci underlying resistance to the METI-I acaricides fenpyroximate, pyridaben and tebufenpyrad without prior hypotheses, we crossed a highly METI-I-resistant strain of T. urticae to a susceptible one, propagated many replicated populations over multiple generations with and without selection by each compound, and performed bulked segregant analysis genetic mapping. Our results showed that while the known H92R target-site mutation was associated with resistance to each compound, a genomic region that included cytochrome P450-reductase (CPR) was associated with resistance to pyridaben and tebufenpyrad. Within CPR, a single nonsynonymous variant distinguished the resistant strain from the sensitive one. Furthermore, a genomic region linked with tebufenpyrad resistance harbored a non-canonical member of the nuclear hormone receptor 96 (NHR96) gene family. This NHR96 gene does not encode a DNA-binding domain (DBD), an uncommon feature in arthropods, and belongs to an expanded family of 47 NHR96 proteins lacking DBDs in T. urticae. Our findings suggest that although cross-resistance to METI-Is involves known detoxification pathways, structural differences in METI-I acaricides have also resulted in resistance mechanisms that are compound-specific.
Keywords
NADH-UBIQUINONE OXIDOREDUCTASE, SULFUR CLUSTER N2, COMPLEX-I, P450 REDUCTASE, KOCH ACARI, ELECTRON-TRANSFER, INHIBITORS, CYTOCHROME-P450, STRAIN, MECHANISMS

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.44 MB
  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 462.85 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Snoeck, Simon, Andre Kurlovs, Sabina Bajda, René Feyereisen, Robert Greenhalgh, Ernesto Villacis-Perez, Olivia Kosterlitz, Wannes Dermauw, Richard M Clark, and Thomas Van Leeuwen. 2019. “High-resolution QTL Mapping in Tetranychus Urticae Reveals Acaricide-specific Responses and Common Target-site Resistance After Selection by Different METI-I Acaricides.” Insect Biochemistry and Molecular Biology 110: 19–33.
APA
Snoeck, S., Kurlovs, A., Bajda, S., Feyereisen, R., Greenhalgh, R., Villacis-Perez, E., Kosterlitz, O., et al. (2019). High-resolution QTL mapping in Tetranychus urticae reveals acaricide-specific responses and common target-site resistance after selection by different METI-I acaricides. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 110, 19–33.
Vancouver
1.
Snoeck S, Kurlovs A, Bajda S, Feyereisen R, Greenhalgh R, Villacis-Perez E, et al. High-resolution QTL mapping in Tetranychus urticae reveals acaricide-specific responses and common target-site resistance after selection by different METI-I acaricides. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY. 2019;110:19–33.
MLA
Snoeck, Simon et al. “High-resolution QTL Mapping in Tetranychus Urticae Reveals Acaricide-specific Responses and Common Target-site Resistance After Selection by Different METI-I Acaricides.” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 110 (2019): 19–33. Print.
@article{8614463,
  abstract     = {Arthropod herbivores cause dramatic crop losses, and frequent pesticide use has led to widespread resistance in numerous species. One such species, the two-spotted spider mite, Tetranychus urticae, is an extreme generalist herbivore and a major worldwide crop pest with a history of rapidly developing resistance to acaricides. Mitochondrial Electron Transport Inhibitors of complex I (METI-Is) have been used extensively in the last 25 years to control T. urticae around the globe, and widespread resistance to each has been documented. METI-I resistance mechanisms in T. urticae are likely complex, as increased metabolism by cytochrome P450 monooxygenases as well as a target-site mutation have been linked with resistance. 
To identify loci underlying resistance to the METI-I acaricides fenpyroximate, pyridaben and tebufenpyrad without prior hypotheses, we crossed a highly METI-I-resistant strain of T. urticae to a susceptible one, propagated many replicated populations over multiple generations with and without selection by each compound, and performed bulked segregant analysis genetic mapping. Our results showed that while the known H92R target-site mutation was associated with resistance to each compound, a genomic region that included cytochrome P450-reductase (CPR) was associated with resistance to pyridaben and tebufenpyrad. Within CPR, a single nonsynonymous variant distinguished the resistant strain from the sensitive one. Furthermore, a genomic region linked with tebufenpyrad resistance harbored a non-canonical member of the nuclear hormone receptor 96 (NHR96) gene family. This NHR96 gene does not encode a DNA-binding domain (DBD), an uncommon feature in arthropods, and belongs to an expanded family of 47 NHR96 proteins lacking DBDs in T. urticae. Our findings suggest that although cross-resistance to METI-Is involves known detoxification pathways, structural differences in METI-I acaricides have also resulted in resistance mechanisms that are compound-specific.},
  author       = {Snoeck, Simon and Kurlovs, Andre and Bajda, Sabina and Feyereisen, René and Greenhalgh, Robert and Villacis-Perez, Ernesto and Kosterlitz, Olivia and Dermauw, Wannes and Clark, Richard M and Van Leeuwen, Thomas},
  issn         = {0965-1748},
  journal      = {INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY},
  keywords     = {NADH-UBIQUINONE OXIDOREDUCTASE,SULFUR CLUSTER N2,COMPLEX-I,P450 REDUCTASE,KOCH ACARI,ELECTRON-TRANSFER,INHIBITORS,CYTOCHROME-P450,STRAIN,MECHANISMS},
  language     = {eng},
  pages        = {19--33},
  title        = {High-resolution QTL mapping in Tetranychus urticae reveals acaricide-specific responses and common target-site resistance after selection by different METI-I acaricides},
  url          = {http://dx.doi.org/10.1016/j.ibmb.2019.04.011},
  volume       = {110},
  year         = {2019},
}

Altmetric
View in Altmetric
Web of Science
Times cited: