A subset of type I conventional dendritic cells controls cutaneous bacterial infections through VEGFα-mediated recruitment of neutrophils
- Author
- Baptiste Janela, Amit A Patel, Mai Chan Lau, Chi Ching Goh, Rasha Msallam, Wan Ting Kong, Michael Fehlings, Sandra Hubert, Josephine Lum, Yannick Simoni, Benoit Malleret, Francesca Zolezzi, Jinmiao Chen, Michael Poidinger, Ansuman T Satpathy, Carlos Briseno, Christian Wohn, Bernard Malissen, Kenneth M Murphy, Alexander A Maini, Leen Vanhoutte (UGent) , Martin Guilliams (UGent) , Emmanuel Vial, Laurent Hennequin, Evan Newell, Lai Guan Ng, Philippe Musette, Simon Yona, Feriel Hacini-Rachinel and Florent Ginhoux
- Organization
- Abstract
- Skin conventional dendritic cells (cDCs) exist as two distinct subsets, cDC1s and cDC2s, which maintain the balance of immunity to pathogens and tolerance to self and microbiota. Here, we examined the roles of dermal cDC1s and cDC2s during bacterial infection, notably Propionibacterium acnes (P. acnes). cDC1s, but not cDC2s, regulated the magnitude of the immune response to P. acnes in the murine dermis by controlling neutrophil recruitment to the inflamed site and survival and function therein. Single-cell mRNA sequencing revealed that this regulation relied on secretion of the cytokine vascular endothelial growth factor alpha (VEGF-alpha) by a minor subset of activated EpCAM(+)CD59(+) Ly-6D(+) cDC1s. Neutrophil recruitment by dermal cDC1s was also observed during S. aureus, bacillus Calmette-Guerin (BCG), or E. coli infection, as well as in a model of bacterial insult in human skin. Thus, skin cDC1s are essential regulators of the innate response in cutaneous immunity and have roles beyond classical antigen presentation.
- Keywords
- HIGH-DIMENSIONAL ANALYSIS, PROPIONIBACTERIUM-ACNES, LANGERHANS CELLS, STEADY-STATE, SKIN, MIGRATION, RESPONSES, IMMUNE, MODEL, IDENTIFICATION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8614350
- MLA
- Janela, Baptiste, et al. “A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils.” IMMUNITY, vol. 50, no. 4, 2019, pp. 1069–83, doi:10.1016/j.immuni.2019.03.001.
- APA
- Janela, B., Patel, A. A., Lau, M. C., Goh, C. C., Msallam, R., Kong, W. T., … Ginhoux, F. (2019). A subset of type I conventional dendritic cells controls cutaneous bacterial infections through VEGFα-mediated recruitment of neutrophils. IMMUNITY, 50(4), 1069–1083. https://doi.org/10.1016/j.immuni.2019.03.001
- Chicago author-date
- Janela, Baptiste, Amit A Patel, Mai Chan Lau, Chi Ching Goh, Rasha Msallam, Wan Ting Kong, Michael Fehlings, et al. 2019. “A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils.” IMMUNITY 50 (4): 1069–83. https://doi.org/10.1016/j.immuni.2019.03.001.
- Chicago author-date (all authors)
- Janela, Baptiste, Amit A Patel, Mai Chan Lau, Chi Ching Goh, Rasha Msallam, Wan Ting Kong, Michael Fehlings, Sandra Hubert, Josephine Lum, Yannick Simoni, Benoit Malleret, Francesca Zolezzi, Jinmiao Chen, Michael Poidinger, Ansuman T Satpathy, Carlos Briseno, Christian Wohn, Bernard Malissen, Kenneth M Murphy, Alexander A Maini, Leen Vanhoutte, Martin Guilliams, Emmanuel Vial, Laurent Hennequin, Evan Newell, Lai Guan Ng, Philippe Musette, Simon Yona, Feriel Hacini-Rachinel, and Florent Ginhoux. 2019. “A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections through VEGFα-Mediated Recruitment of Neutrophils.” IMMUNITY 50 (4): 1069–1083. doi:10.1016/j.immuni.2019.03.001.
- Vancouver
- 1.Janela B, Patel AA, Lau MC, Goh CC, Msallam R, Kong WT, et al. A subset of type I conventional dendritic cells controls cutaneous bacterial infections through VEGFα-mediated recruitment of neutrophils. IMMUNITY. 2019;50(4):1069–83.
- IEEE
- [1]B. Janela et al., “A subset of type I conventional dendritic cells controls cutaneous bacterial infections through VEGFα-mediated recruitment of neutrophils,” IMMUNITY, vol. 50, no. 4, pp. 1069–1083, 2019.
@article{8614350,
abstract = {{Skin conventional dendritic cells (cDCs) exist as two distinct subsets, cDC1s and cDC2s, which maintain the balance of immunity to pathogens and tolerance to self and microbiota. Here, we examined the roles of dermal cDC1s and cDC2s during bacterial infection, notably Propionibacterium acnes (P. acnes). cDC1s, but not cDC2s, regulated the magnitude of the immune response to P. acnes in the murine dermis by controlling neutrophil recruitment to the inflamed site and survival and function therein. Single-cell mRNA sequencing revealed that this regulation relied on secretion of the cytokine vascular endothelial growth factor alpha (VEGF-alpha) by a minor subset of activated EpCAM(+)CD59(+) Ly-6D(+) cDC1s. Neutrophil recruitment by dermal cDC1s was also observed during S. aureus, bacillus Calmette-Guerin (BCG), or E. coli infection, as well as in a model of bacterial insult in human skin. Thus, skin cDC1s are essential regulators of the innate response in cutaneous immunity and have roles beyond classical antigen presentation.}},
author = {{Janela, Baptiste and Patel, Amit A and Lau, Mai Chan and Goh, Chi Ching and Msallam, Rasha and Kong, Wan Ting and Fehlings, Michael and Hubert, Sandra and Lum, Josephine and Simoni, Yannick and Malleret, Benoit and Zolezzi, Francesca and Chen, Jinmiao and Poidinger, Michael and Satpathy, Ansuman T and Briseno, Carlos and Wohn, Christian and Malissen, Bernard and Murphy, Kenneth M and Maini, Alexander A and Vanhoutte, Leen and Guilliams, Martin and Vial, Emmanuel and Hennequin, Laurent and Newell, Evan and Ng, Lai Guan and Musette, Philippe and Yona, Simon and Hacini-Rachinel, Feriel and Ginhoux, Florent}},
issn = {{1074-7613}},
journal = {{IMMUNITY}},
keywords = {{HIGH-DIMENSIONAL ANALYSIS,PROPIONIBACTERIUM-ACNES,LANGERHANS CELLS,STEADY-STATE,SKIN,MIGRATION,RESPONSES,IMMUNE,MODEL,IDENTIFICATION}},
language = {{eng}},
number = {{4}},
pages = {{1069--1083}},
title = {{A subset of type I conventional dendritic cells controls cutaneous bacterial infections through VEGFα-mediated recruitment of neutrophils}},
url = {{http://doi.org/10.1016/j.immuni.2019.03.001}},
volume = {{50}},
year = {{2019}},
}
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