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A subset of type I conventional dendritic cells controls cutaneous bacterial infections through VEGFα-mediated recruitment of neutrophils

(2019) IMMUNITY. 50(4). p.1069-1083
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Abstract
Skin conventional dendritic cells (cDCs) exist as two distinct subsets, cDC1s and cDC2s, which maintain the balance of immunity to pathogens and tolerance to self and microbiota. Here, we examined the roles of dermal cDC1s and cDC2s during bacterial infection, notably Propionibacterium acnes (P. acnes). cDC1s, but not cDC2s, regulated the magnitude of the immune response to P. acnes in the murine dermis by controlling neutrophil recruitment to the inflamed site and survival and function therein. Single-cell mRNA sequencing revealed that this regulation relied on secretion of the cytokine vascular endothelial growth factor alpha (VEGF-alpha) by a minor subset of activated EpCAM(+)CD59(+) Ly-6D(+) cDC1s. Neutrophil recruitment by dermal cDC1s was also observed during S. aureus, bacillus Calmette-Guerin (BCG), or E. coli infection, as well as in a model of bacterial insult in human skin. Thus, skin cDC1s are essential regulators of the innate response in cutaneous immunity and have roles beyond classical antigen presentation.
Keywords
HIGH-DIMENSIONAL ANALYSIS, PROPIONIBACTERIUM-ACNES, LANGERHANS CELLS, STEADY-STATE, SKIN, MIGRATION, RESPONSES, IMMUNE, MODEL, IDENTIFICATION

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Chicago
Janela, Baptiste, Amit A Patel, Mai Chan Lau, Chi Ching Goh, Rasha Msallam, Wan Ting Kong, Michael Fehlings, et al. 2019. “A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections Through VEGFα-mediated Recruitment of Neutrophils.” Immunity 50 (4): 1069–1083.
APA
Janela, B., Patel, A. A., Lau, M. C., Goh, C. C., Msallam, R., Kong, W. T., Fehlings, M., et al. (2019). A subset of type I conventional dendritic cells controls cutaneous bacterial infections through VEGFα-mediated recruitment of neutrophils. IMMUNITY, 50(4), 1069–1083.
Vancouver
1.
Janela B, Patel AA, Lau MC, Goh CC, Msallam R, Kong WT, et al. A subset of type I conventional dendritic cells controls cutaneous bacterial infections through VEGFα-mediated recruitment of neutrophils. IMMUNITY. 2019;50(4):1069–83.
MLA
Janela, Baptiste et al. “A Subset of Type I Conventional Dendritic Cells Controls Cutaneous Bacterial Infections Through VEGFα-mediated Recruitment of Neutrophils.” IMMUNITY 50.4 (2019): 1069–1083. Print.
@article{8614350,
  abstract     = {Skin conventional dendritic cells (cDCs) exist as two distinct subsets, cDC1s and cDC2s, which maintain the balance of immunity to pathogens and tolerance to self and microbiota. Here, we examined the roles of dermal cDC1s and cDC2s during bacterial infection, notably Propionibacterium acnes (P. acnes). cDC1s, but not cDC2s, regulated the magnitude of the immune response to P. acnes in the murine dermis by controlling neutrophil recruitment to the inflamed site and survival and function therein. Single-cell mRNA sequencing revealed that this regulation relied on secretion of the cytokine vascular endothelial growth factor alpha (VEGF-alpha) by a minor subset of activated EpCAM(+)CD59(+) Ly-6D(+) cDC1s. Neutrophil recruitment by dermal cDC1s was also observed during S. aureus, bacillus Calmette-Guerin (BCG), or E. coli infection, as well as in a model of bacterial insult in human skin. Thus, skin cDC1s are essential regulators of the innate response in cutaneous immunity and have roles beyond classical antigen presentation.},
  author       = {Janela, Baptiste and Patel, Amit A and Lau, Mai Chan and Goh, Chi Ching and Msallam, Rasha and Kong, Wan Ting and Fehlings, Michael and Hubert, Sandra and Lum, Josephine and Simoni, Yannick and Malleret, Benoit and Zolezzi, Francesca and Chen, Jinmiao and Poidinger, Michael and Satpathy, Ansuman T and Briseno, Carlos and Wohn, Christian and Malissen, Bernard and Murphy, Kenneth M and Maini, Alexander A and Vanhoutte, Leen and Guilliams, Martin and Vial, Emmanuel and Hennequin, Laurent and Newell, Evan and Ng, Lai Guan and Musette, Philippe and Yona, Simon and Hacini-Rachinel, Feriel and Ginhoux, Florent},
  issn         = {1074-7613},
  journal      = {IMMUNITY},
  language     = {eng},
  number       = {4},
  pages        = {1069--1083},
  title        = {A subset of type I conventional dendritic cells controls cutaneous bacterial infections through VEGF\ensuremath{\alpha}-mediated recruitment of neutrophils},
  url          = {http://dx.doi.org/10.1016/j.immuni.2019.03.001},
  volume       = {50},
  year         = {2019},
}

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