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Optimizing pear micropropagation and rooting with light emitting diodes and trans-cinnamic acid

(2019) PLANT GROWTH REGULATION. 88(2). p.173-180
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Abstract
Meta-Topolin riboside was selected as cytokinin for these experiments. The effects of combinations of monochromatic blue, red and far red LED light were compared to fluorescent light during the micropropagation and rooting of a recalcitrant pear (Pyrus communis L.). During the micropropagation phase, red light gave some particular advantages: maximal shoot length and leaf area were obtained. Under blue light, callus weight quadrupled compared to fluorescent light. Although far red was advantageous for shoot number, shoot quality was inferior because of hyperhydricity and chlorosis as indicated by a low Chlorophyll a+b and carotenoid content. The smallest leaf area was detected under fluorescent light. Blue+red showed significant improvements. Shoot cluster weight and shoot weight/callus weight' ratio was maximal, as well as shoot number and shoot length. Leaves were also dark green, showing a maximal Chlorophyll a+b and carotenoids content. Adventitious rooting of in vitro cultured pear plants was highly affected by different light spectra and the addition of a new rooting compound: trans-cinnamic acid. Without trans-cinnamic acid, limited rooting was observed under red, blue and blue+red light. In combination with trans-cinnamic acid, 100% rooting was achieved under red light.
Keywords
LED, Shoot multiplication, Auxin, Pyrus, In vitro, IN-VITRO GROWTH, RED-LIGHT, SHOOT REGENERATION, FAR-RED, MORPHOGENESIS, QUALITY, PLANT, IRRADIATION, CULTIVARS, LEDS

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MLA
Lotfi, Mariem, Messaoud Mars, and Stefaan Werbrouck. “Optimizing Pear Micropropagation and Rooting with Light Emitting Diodes and Trans-cinnamic Acid.” PLANT GROWTH REGULATION 88.2 (2019): 173–180. Print.
APA
Lotfi, M., Mars, M., & Werbrouck, S. (2019). Optimizing pear micropropagation and rooting with light emitting diodes and trans-cinnamic acid. PLANT GROWTH REGULATION, 88(2), 173–180.
Chicago author-date
Lotfi, Mariem, Messaoud Mars, and Stefaan Werbrouck. 2019. “Optimizing Pear Micropropagation and Rooting with Light Emitting Diodes and Trans-cinnamic Acid.” Plant Growth Regulation 88 (2): 173–180.
Chicago author-date (all authors)
Lotfi, Mariem, Messaoud Mars, and Stefaan Werbrouck. 2019. “Optimizing Pear Micropropagation and Rooting with Light Emitting Diodes and Trans-cinnamic Acid.” Plant Growth Regulation 88 (2): 173–180.
Vancouver
1.
Lotfi M, Mars M, Werbrouck S. Optimizing pear micropropagation and rooting with light emitting diodes and trans-cinnamic acid. PLANT GROWTH REGULATION. 2019;88(2):173–80.
IEEE
[1]
M. Lotfi, M. Mars, and S. Werbrouck, “Optimizing pear micropropagation and rooting with light emitting diodes and trans-cinnamic acid,” PLANT GROWTH REGULATION, vol. 88, no. 2, pp. 173–180, 2019.
@article{8613407,
  abstract     = {Meta-Topolin riboside was selected as cytokinin for these experiments. The effects of combinations of monochromatic blue, red and far red LED light were compared to fluorescent light during the micropropagation and rooting of a recalcitrant pear (Pyrus communis L.). During the micropropagation phase, red light gave some particular advantages: maximal shoot length and leaf area were obtained. Under blue light, callus weight quadrupled compared to fluorescent light. Although far red was advantageous for shoot number, shoot quality was inferior because of hyperhydricity and chlorosis as indicated by a low Chlorophyll a+b and carotenoid content. The smallest leaf area was detected under fluorescent light. Blue+red showed significant improvements. Shoot cluster weight and shoot weight/callus weight' ratio was maximal, as well as shoot number and shoot length. Leaves were also dark green, showing a maximal Chlorophyll a+b and carotenoids content. Adventitious rooting of in vitro cultured pear plants was highly affected by different light spectra and the addition of a new rooting compound: trans-cinnamic acid. Without trans-cinnamic acid, limited rooting was observed under red, blue and blue+red light. In combination with trans-cinnamic acid, 100% rooting was achieved under red light.},
  author       = {Lotfi, Mariem and Mars, Messaoud and Werbrouck, Stefaan},
  issn         = {0167-6903},
  journal      = {PLANT GROWTH REGULATION},
  keywords     = {LED,Shoot multiplication,Auxin,Pyrus,In vitro,IN-VITRO GROWTH,RED-LIGHT,SHOOT REGENERATION,FAR-RED,MORPHOGENESIS,QUALITY,PLANT,IRRADIATION,CULTIVARS,LEDS},
  language     = {eng},
  number       = {2},
  pages        = {173--180},
  title        = {Optimizing pear micropropagation and rooting with light emitting diodes and trans-cinnamic acid},
  url          = {http://dx.doi.org/10.1007/s10725-019-00498-y},
  volume       = {88},
  year         = {2019},
}

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