Advanced search
1 file | 1.53 MB Add to list

Occurence and characterisation of biofilms in drinking water systems of broiler houses

Author
Organization
Abstract
Background: Water quality in the drinking water system (DWS) plays an important role in the general health and performance of broiler chickens. Conditions in the DWS of broilers are ideal for microbial biofilm formation. Since pathogens might reside within these biofilms, they serve as potential source of waterborne transmission of pathogens to livestock and humans. Knowledge about the presence, importance and composition of biofilms in the DWS of broilers is largely missing. In this study, we therefore aim to monitor the occurrence, and chemically and microbiologically characterise biofilms in the DWS of five broiler farms. Results: The bacterial load after disinfection in DWSs was assessed by sampling with a flocked swab followed by enumerations of total aerobic flora (TAC) and Pseudomonas spp. The dominant flora was identified and their biofilm-forming capacity was evaluated. Also, proteins, carbohydrates and uronic acids were quantified to analyse the presence of extracellular polymeric substances of biofilms. Despite disinfection of the water and the DWS, average TAC was 6.031.53 log CFU/20cm(2). Enumerations for Pseudomonas spp. were on average 0.88 log CFU/20cm(2) lower. The most identified dominant species from TAC were Stenotrophomonas maltophilia, Pseudomonas geniculata and Pseudomonas aeruginosa. However at species level, most of the identified microorganisms were farm specific. Almost all the isolates belonging to the three most abundant species were strong biofilm producers. Overall, 92% of all tested microorganisms were able to form biofilm under lab conditions. Furthermore, 63% of the DWS surfaces appeared to be contaminated with microorganisms combined with at least one of the analysed chemical components, which is indicative for the presence of biofilm. Conclusions: Stenotrophomonas maltophilia, Pseudomonas geniculata and Pseudomonas aeruginosa are considered as opportunistic pathogens and could consequently be a potential risk for animal health. Additionally, the biofilm-forming capacity of these organisms could promote attachment of other pathogens such as Campylobacter spp. and Salmonella spp.
Keywords
Biofilm, Broiler, Drinking water system, Pseudomonas spp., Stenotrophomonas maltophilia, RIBOSOMAL-RNA GENE, CAMPYLOBACTER-JEJUNI, PSEUDOMONAS-AERUGINOSA, STAPHYLOCOCCUS-AUREUS, ANTIBIOTIC-RESISTANCE, SALMONELLA-ENTERICA, POULTRY, SEQUENCE, QUANTIFICATION, IDENTIFICATION

Downloads

  • Maes et al-2019-BMC Microbiology.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 1.53 MB

Citation

Please use this url to cite or link to this publication:

MLA
Maes, Sharon et al. “Occurence and Characterisation of Biofilms in Drinking Water Systems of Broiler Houses.” BMC MICROBIOLOGY 19 (2019): n. pag. Print.
APA
Maes, Sharon, Vackier, T., Huu, S. N., Heyndrickx, M., Steenackers, H., Sampers, I., Raes, K., et al. (2019). Occurence and characterisation of biofilms in drinking water systems of broiler houses. BMC MICROBIOLOGY, 19.
Chicago author-date
Maes, Sharon, Thijs Vackier, Son Nguyen Huu, Marc Heyndrickx, Hans Steenackers, Imca Sampers, Katleen Raes, Alex Verplaetse, and Koen De Reu. 2019. “Occurence and Characterisation of Biofilms in Drinking Water Systems of Broiler Houses.” Bmc Microbiology 19.
Chicago author-date (all authors)
Maes, Sharon, Thijs Vackier, Son Nguyen Huu, Marc Heyndrickx, Hans Steenackers, Imca Sampers, Katleen Raes, Alex Verplaetse, and Koen De Reu. 2019. “Occurence and Characterisation of Biofilms in Drinking Water Systems of Broiler Houses.” Bmc Microbiology 19.
Vancouver
1.
Maes S, Vackier T, Huu SN, Heyndrickx M, Steenackers H, Sampers I, et al. Occurence and characterisation of biofilms in drinking water systems of broiler houses. BMC MICROBIOLOGY. 2019;19.
IEEE
[1]
S. Maes et al., “Occurence and characterisation of biofilms in drinking water systems of broiler houses,” BMC MICROBIOLOGY, vol. 19, 2019.
@article{8612977,
  abstract     = {{Background: Water quality in the drinking water system (DWS) plays an important role in the general health and performance of broiler chickens. Conditions in the DWS of broilers are ideal for microbial biofilm formation. Since pathogens might reside within these biofilms, they serve as potential source of waterborne transmission of pathogens to livestock and humans. Knowledge about the presence, importance and composition of biofilms in the DWS of broilers is largely missing. In this study, we therefore aim to monitor the occurrence, and chemically and microbiologically characterise biofilms in the DWS of five broiler farms. 
Results: The bacterial load after disinfection in DWSs was assessed by sampling with a flocked swab followed by enumerations of total aerobic flora (TAC) and Pseudomonas spp. The dominant flora was identified and their biofilm-forming capacity was evaluated. Also, proteins, carbohydrates and uronic acids were quantified to analyse the presence of extracellular polymeric substances of biofilms. Despite disinfection of the water and the DWS, average TAC was 6.031.53 log CFU/20cm(2). Enumerations for Pseudomonas spp. were on average 0.88 log CFU/20cm(2) lower. The most identified dominant species from TAC were Stenotrophomonas maltophilia, Pseudomonas geniculata and Pseudomonas aeruginosa. However at species level, most of the identified microorganisms were farm specific. Almost all the isolates belonging to the three most abundant species were strong biofilm producers. Overall, 92% of all tested microorganisms were able to form biofilm under lab conditions. Furthermore, 63% of the DWS surfaces appeared to be contaminated with microorganisms combined with at least one of the analysed chemical components, which is indicative for the presence of biofilm. 
Conclusions: Stenotrophomonas maltophilia, Pseudomonas geniculata and Pseudomonas aeruginosa are considered as opportunistic pathogens and could consequently be a potential risk for animal health. Additionally, the biofilm-forming capacity of these organisms could promote attachment of other pathogens such as Campylobacter spp. and Salmonella spp.}},
  articleno    = {{77}},
  author       = {{Maes, Sharon and Vackier, Thijs and Huu, Son Nguyen and Heyndrickx, Marc and Steenackers, Hans and Sampers, Imca and Raes, Katleen and Verplaetse, Alex and De Reu, Koen}},
  issn         = {{1471-2180}},
  journal      = {{BMC MICROBIOLOGY}},
  keywords     = {{Biofilm,Broiler,Drinking water system,Pseudomonas spp.,Stenotrophomonas maltophilia,RIBOSOMAL-RNA GENE,CAMPYLOBACTER-JEJUNI,PSEUDOMONAS-AERUGINOSA,STAPHYLOCOCCUS-AUREUS,ANTIBIOTIC-RESISTANCE,SALMONELLA-ENTERICA,POULTRY,SEQUENCE,QUANTIFICATION,IDENTIFICATION}},
  language     = {{eng}},
  pages        = {{15}},
  title        = {{Occurence and characterisation of biofilms in drinking water systems of broiler houses}},
  url          = {{http://dx.doi.org/10.1186/s12866-019-1451-5}},
  volume       = {{19}},
  year         = {{2019}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: