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The MYB transcription factor Emission of Methyl Anthranilate 1 stimulates emission of methyl anthranilate from Medicago truncatula hairy roots

(2019) PLANT JOURNAL. 99(4). p.637-654
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Abstract
Plants respond to herbivore or pathogen attacks by activating specific defense programs that include the production of bioactive specialized metabolites to eliminate or deter the attackers. Volatiles play an important role in the interaction of a plant with its environment. Through transcript profiling of jasmonate‐elicited Medicago truncatula cells we identified Emission of Methyl Anthranilate (EMA) 1, a MYB transcription factor that is involved in the emission of the volatile compound methyl anthranilate when expressed in M. truncatula hairy roots, giving them a fruity scent. RNA‐Seq analysis of the fragrant roots revealed the upregulation of a methyltransferase that was subsequently characterized to catalyze the O‐methylation of anthranilic acid and was hence named M. truncatula Anthranilic Acid Methyl Transferase (MtAAMT) 1. Given that direct activation of the MtAAMT1 promoter by EMA1 could not be unambiguously demonstrated, we further probed the RNA‐Seq data and identified the repressor protein M. truncatula Plant AT‐rich sequence and Zinc‐binding (MtPLATZ) 1. EMA1 binds a tandem repeat of the ACCTAAC motif in the MtPLATZ1 promoter to transactivate gene expression. Overexpression of MtPLATZ1 in transgenic M. truncatula hairy roots led to transcriptional silencing of EMA1, indicating that MtPLATZ1 may be part of a negative feedback loop to control the expression of EMA1. Finally, exogenous methyl anthranilate application boosted EMA1 and MtAAMT1 expression dramatically, thus also revealing a positive amplification loop. Such positive and negative feedback loops seem to be a norm rather than an exception in the regulation of plant specialized metabolism.
Keywords
volatile organic compounds, MYB transcription factor, PLATZ transcription factor, methyl anthranilate, methyl transferase, Medicago truncatula, jasmonate signaling, FLORAL SCENT PRODUCTION, TRITERPENE SAPONIN BIOSYNTHESIS, ACID CARBOXYL METHYLTRANSFERASE, DNA-BINDING SPECIFICITIES, R2R3-MYB GENE FAMILY, PLANT DEFENSE, JASMONATES BIOSYNTHESIS, SIGNAL-TRANSDUCTION, ARABIDOPSIS, PROTEINS

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Citation

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MLA
Pollier, Jacob, et al. “The MYB Transcription Factor Emission of Methyl Anthranilate 1 Stimulates Emission of Methyl Anthranilate from Medicago Truncatula Hairy Roots.” PLANT JOURNAL, vol. 99, no. 4, 2019, pp. 637–54, doi:10.1111/tpj.14347.
APA
Pollier, J., De Geyter, N., Moses, T., Boachon, B., Franco Zorrilla, J. M., Bai, Y., … Goossens, A. (2019). The MYB transcription factor Emission of Methyl Anthranilate 1 stimulates emission of methyl anthranilate from Medicago truncatula hairy roots. PLANT JOURNAL, 99(4), 637–654. https://doi.org/10.1111/tpj.14347
Chicago author-date
Pollier, Jacob, Nathan De Geyter, Tessa Moses, Benoît Boachon, José Manuel Franco Zorrilla, Yuechen Bai, Elia Lacchini, et al. 2019. “The MYB Transcription Factor Emission of Methyl Anthranilate 1 Stimulates Emission of Methyl Anthranilate from Medicago Truncatula Hairy Roots.” PLANT JOURNAL 99 (4): 637–54. https://doi.org/10.1111/tpj.14347.
Chicago author-date (all authors)
Pollier, Jacob, Nathan De Geyter, Tessa Moses, Benoît Boachon, José Manuel Franco Zorrilla, Yuechen Bai, Elia Lacchini, Azra Gholami, Robin Vanden Bossche, Danièle Werck‐Reichhart, Sofie Goormachtig, and Alain Goossens. 2019. “The MYB Transcription Factor Emission of Methyl Anthranilate 1 Stimulates Emission of Methyl Anthranilate from Medicago Truncatula Hairy Roots.” PLANT JOURNAL 99 (4): 637–654. doi:10.1111/tpj.14347.
Vancouver
1.
Pollier J, De Geyter N, Moses T, Boachon B, Franco Zorrilla JM, Bai Y, et al. The MYB transcription factor Emission of Methyl Anthranilate 1 stimulates emission of methyl anthranilate from Medicago truncatula hairy roots. PLANT JOURNAL. 2019;99(4):637–54.
IEEE
[1]
J. Pollier et al., “The MYB transcription factor Emission of Methyl Anthranilate 1 stimulates emission of methyl anthranilate from Medicago truncatula hairy roots,” PLANT JOURNAL, vol. 99, no. 4, pp. 637–654, 2019.
@article{8612797,
  abstract     = {Plants respond to herbivore or pathogen attacks by activating specific defense programs that include the production of bioactive specialized metabolites to eliminate or deter the attackers. Volatiles play an important role in the interaction of a plant with its environment. Through transcript profiling of jasmonate‐elicited Medicago truncatula cells we identified Emission of Methyl Anthranilate (EMA) 1, a MYB transcription factor that is involved in the emission of the volatile compound methyl anthranilate when expressed in M. truncatula hairy roots, giving them a fruity scent. RNA‐Seq analysis of the fragrant roots revealed the upregulation of a methyltransferase that was subsequently characterized to catalyze the O‐methylation of anthranilic acid and was hence named M. truncatula Anthranilic Acid Methyl Transferase (MtAAMT) 1. Given that direct activation of the MtAAMT1 promoter by EMA1 could not be unambiguously demonstrated, we further probed the RNA‐Seq data and identified the repressor protein M. truncatula Plant AT‐rich sequence and Zinc‐binding (MtPLATZ) 1. EMA1 binds a tandem repeat of the ACCTAAC motif in the MtPLATZ1 promoter to transactivate gene expression. Overexpression of MtPLATZ1 in transgenic M. truncatula hairy roots led to transcriptional silencing of EMA1, indicating that MtPLATZ1 may be part of a negative feedback loop to control the expression of EMA1. Finally, exogenous methyl anthranilate application boosted EMA1 and MtAAMT1 expression dramatically, thus also revealing a positive amplification loop. Such positive and negative feedback loops seem to be a norm rather than an exception in the regulation of plant specialized metabolism.},
  author       = {Pollier, Jacob and De Geyter, Nathan and Moses, Tessa and Boachon, Benoît and Franco Zorrilla, José Manuel and Bai, Yuechen and Lacchini, Elia and Gholami, Azra and Vanden Bossche, Robin and Werck‐Reichhart, Danièle and Goormachtig, Sofie and Goossens, Alain},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keywords     = {volatile organic compounds,MYB transcription factor,PLATZ transcription factor,methyl anthranilate,methyl transferase,Medicago truncatula,jasmonate signaling,FLORAL SCENT PRODUCTION,TRITERPENE SAPONIN BIOSYNTHESIS,ACID CARBOXYL METHYLTRANSFERASE,DNA-BINDING SPECIFICITIES,R2R3-MYB GENE FAMILY,PLANT DEFENSE,JASMONATES BIOSYNTHESIS,SIGNAL-TRANSDUCTION,ARABIDOPSIS,PROTEINS},
  language     = {eng},
  number       = {4},
  pages        = {637--654},
  title        = {The MYB transcription factor Emission of Methyl Anthranilate 1 stimulates emission of methyl anthranilate from Medicago truncatula hairy roots},
  url          = {http://dx.doi.org/10.1111/tpj.14347},
  volume       = {99},
  year         = {2019},
}

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