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N-Linked glycosylation and near-infrared spectroscopy in the diagnosis of prostate cancer

Tijl Vermassen (UGent) , Sander De Bruyne (UGent) , Jonas Himpe (UGent) , Nicolaas Lumen (UGent) , Nico Callewaert (UGent) , Sylvie Rottey (UGent) and Joris Delanghe (UGent)
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Abstract
Background: Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading. As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis. Methods: Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained. The fresh-frozen tissue was dispersed and a tissue N-glycosylation profile was determined. Consequently, the formalin-fixed paraffin-embedded slides were analyzed using NIR spectroscopy. A comparison was made between the benign and malignant tissue, and between the various Gleason scores. Results: A difference was observed for the tissue of N-glycosylation between the benign and malignant tissue. These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001). These differences were also present between various Gleason scores. In addition, the NIR spectra revealed changes between the benign and malignant tissue in several regions. Moreover, spectral ranges of 1055-1065 nm and 1450-1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195). Conclusions: We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores. These changes can be correlated to the changes observed in the NIR spectra. This could possibly further improve the histological assessment of PCa diagnosis, although further method validation is needed.
Keywords
Benign prostate hyperplasia, gleason score, infrared spectroscopy, N-glycosylation, prostate cancer, STEM-CELLS, ANTIGEN, BIOMARKERS

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MLA
Vermassen, Tijl, et al. “N-Linked Glycosylation and near-Infrared Spectroscopy in the Diagnosis of Prostate Cancer.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 7, 2019.
APA
Vermassen, T., De Bruyne, S., Himpe, J., Lumen, N., Callewaert, N., Rottey, S., & Delanghe, J. (2019). N-Linked glycosylation and near-infrared spectroscopy in the diagnosis of prostate cancer. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(7).
Chicago author-date
Vermassen, Tijl, Sander De Bruyne, Jonas Himpe, Nicolaas Lumen, Nico Callewaert, Sylvie Rottey, and Joris Delanghe. 2019. “N-Linked Glycosylation and near-Infrared Spectroscopy in the Diagnosis of Prostate Cancer.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20 (7).
Chicago author-date (all authors)
Vermassen, Tijl, Sander De Bruyne, Jonas Himpe, Nicolaas Lumen, Nico Callewaert, Sylvie Rottey, and Joris Delanghe. 2019. “N-Linked Glycosylation and near-Infrared Spectroscopy in the Diagnosis of Prostate Cancer.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20 (7).
Vancouver
1.
Vermassen T, De Bruyne S, Himpe J, Lumen N, Callewaert N, Rottey S, et al. N-Linked glycosylation and near-infrared spectroscopy in the diagnosis of prostate cancer. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2019;20(7).
IEEE
[1]
T. Vermassen et al., “N-Linked glycosylation and near-infrared spectroscopy in the diagnosis of prostate cancer,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 20, no. 7, 2019.
@article{8612017,
  abstract     = {Background: Performing a prostate biopsy is the most robust and reliable way to diagnose prostate cancer (PCa), and to determine the disease grading. As little to no biochemical markers for prostate tissue exist, we explored the possibilities of tissue N-glycosylation and near-infrared spectroscopy (NIR) in PCa diagnosis.
Methods: Tissue specimens from 100 patients (benign prostate hyperplasia (BPH), n = 50; and PCa, n = 50) were obtained. The fresh-frozen tissue was dispersed and a tissue N-glycosylation profile was determined. Consequently, the formalin-fixed paraffin-embedded slides were analyzed using NIR spectroscopy. A comparison was made between the benign and malignant tissue, and between the various Gleason scores.
Results: A difference was observed for the tissue of N-glycosylation between the benign and malignant tissue. These differences were located in the fycosylation ratios and the total amount of bi- and tetra-antennary structures (all p < 0.0001). These differences were also present between various Gleason scores. In addition, the NIR spectra revealed changes between the benign and malignant tissue in several regions. Moreover, spectral ranges of 1055-1065 nm and 1450-1460 nm were significantly different between the Gleason scores (p = 0.0042 and p = 0.0195).
Conclusions: We have demonstrated biochemical changes in the N-glycan profile of prostate tissue, which allows for the distinction between malignant and benign tissue, as well as between various Gleason scores. These changes can be correlated to the changes observed in the NIR spectra. This could possibly further improve the histological assessment of PCa diagnosis, although further method validation is needed.},
  articleno    = {1592},
  author       = {Vermassen, Tijl and De Bruyne, Sander and Himpe, Jonas and Lumen, Nicolaas and Callewaert, Nico and Rottey, Sylvie and Delanghe, Joris},
  issn         = {1422-0067},
  journal      = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
  keywords     = {Benign prostate hyperplasia,gleason score,infrared spectroscopy,N-glycosylation,prostate cancer,STEM-CELLS,ANTIGEN,BIOMARKERS},
  language     = {eng},
  number       = {7},
  pages        = {13},
  title        = {N-Linked glycosylation and near-infrared spectroscopy in the diagnosis of prostate cancer},
  url          = {http://dx.doi.org/10.3390/ijms20071592},
  volume       = {20},
  year         = {2019},
}

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