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Clinical determination of folates : recent analytical strategies and challenges

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Abstract
Since the introduction of liquid chromatography tandem mass spectrometry in clinical laboratories, folate analysis has shifted from microbiological or protein-binding assays to chromatographic methods. Now, it is possible to sensitively and selectively determine several folate species in clinical samples where only a total folate content could be quantified using a microbiological or a binding assay. Although several chromatographic methods have been developed, validated, and published, interlaboratory variability limits the comparability of the results. In this review, we provide an overview of the latest strategies for sampling, sample treatment, and analysis and how these may influence the final analytical result. Among the variables covered are the effect of pH, temperature, and storage and the use of antioxidants and anticoagulants on analyte stability. In addition, we highlight the importance of correct assay calibration and the use of (labeled) certified reference materials in order to obtain correct and comparable results among different laboratories.
Keywords
Folate, Plasma, Serum, Red blood cell, Chromatography, Mass spectrometry, TANDEM MASS-SPECTROMETRY, PERFORMANCE LIQUID-CHROMATOGRAPHY, DRIED BLOOD SPOTS, SOLID-PHASE EXTRACTION, FOLIC-ACID, SERUM FOLATE, HUMAN PLASMA, HIGH-THROUGHPUT, LC-MS/MS, 5-METHYLTETRAHYDROFOLIC ACID

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MLA
Verstraete, Jana, et al. “Clinical Determination of Folates : Recent Analytical Strategies and Challenges.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 411, no. 19, 2019, pp. 4383–99, doi:10.1007/s00216-019-01574-y.
APA
Verstraete, J., Kiekens, F., Strobbe, S., De Steur, H., Gellynck, X., Van Der Straeten, D., & Stove, C. (2019). Clinical determination of folates : recent analytical strategies and challenges. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 411(19), 4383–4399. https://doi.org/10.1007/s00216-019-01574-y
Chicago author-date
Verstraete, Jana, Filip Kiekens, Simon Strobbe, Hans De Steur, Xavier Gellynck, Dominique Van Der Straeten, and Christophe Stove. 2019. “Clinical Determination of Folates : Recent Analytical Strategies and Challenges.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411 (19): 4383–99. https://doi.org/10.1007/s00216-019-01574-y.
Chicago author-date (all authors)
Verstraete, Jana, Filip Kiekens, Simon Strobbe, Hans De Steur, Xavier Gellynck, Dominique Van Der Straeten, and Christophe Stove. 2019. “Clinical Determination of Folates : Recent Analytical Strategies and Challenges.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411 (19): 4383–4399. doi:10.1007/s00216-019-01574-y.
Vancouver
1.
Verstraete J, Kiekens F, Strobbe S, De Steur H, Gellynck X, Van Der Straeten D, et al. Clinical determination of folates : recent analytical strategies and challenges. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2019;411(19):4383–99.
IEEE
[1]
J. Verstraete et al., “Clinical determination of folates : recent analytical strategies and challenges,” ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 411, no. 19, pp. 4383–4399, 2019.
@article{8611409,
  abstract     = {Since the introduction of liquid chromatography tandem mass spectrometry in clinical laboratories, folate analysis has shifted from microbiological or protein-binding assays to chromatographic methods. Now, it is possible to sensitively and selectively determine several folate species in clinical samples where only a total folate content could be quantified using a microbiological or a binding assay. Although several chromatographic methods have been developed, validated, and published, interlaboratory variability limits the comparability of the results. In this review, we provide an overview of the latest strategies for sampling, sample treatment, and analysis and how these may influence the final analytical result. Among the variables covered are the effect of pH, temperature, and storage and the use of antioxidants and anticoagulants on analyte stability. In addition, we highlight the importance of correct assay calibration and the use of (labeled) certified reference materials in order to obtain correct and comparable results among different laboratories.},
  author       = {Verstraete, Jana and Kiekens, Filip and Strobbe, Simon and De Steur, Hans and Gellynck, Xavier and Van Der Straeten, Dominique and Stove, Christophe},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keywords     = {Folate,Plasma,Serum,Red blood cell,Chromatography,Mass spectrometry,TANDEM MASS-SPECTROMETRY,PERFORMANCE LIQUID-CHROMATOGRAPHY,DRIED BLOOD SPOTS,SOLID-PHASE EXTRACTION,FOLIC-ACID,SERUM FOLATE,HUMAN PLASMA,HIGH-THROUGHPUT,LC-MS/MS,5-METHYLTETRAHYDROFOLIC ACID},
  language     = {eng},
  number       = {19},
  pages        = {4383--4399},
  title        = {Clinical determination of folates : recent analytical strategies and challenges},
  url          = {http://dx.doi.org/10.1007/s00216-019-01574-y},
  volume       = {411},
  year         = {2019},
}

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