Challenges for labeling and longitudinal tracking of adoptively transferred autoreactive T lymphocytes in an experimental type-1 diabetes model
- Author
- Shweta Saini, Hannelie Korf, Sayuan Liang, Rein Verbeke (UGent) , Bella Manshian, Koen Raemdonck (UGent) , Ine Lentacker (UGent) , Conny Gysemans, Stefaan De Smedt (UGent) and Uwe Himmelreich
- Organization
- Abstract
- Objective: Tracking the autoreactive T-cell migration in the pancreatic region after labeling with fluorinated nanoparticles (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[3-(2-pyridyldithio)propionate]-perfluoro-15-crown-5-ether nanoparticles, PDP-PFCE NPs) in a diabetic murine model using F-19 MRI. Materials and methods: Synthesis of novel PDP-PFCE fluorine tracer was performed for in vitro labeling of T cells. Labeling conditions were optimized using different PDP-PFCE NPs concentrations. For in vivo F-19 MRI, mice were longitudinally followed after adoptive transfer of activated, autoreactive, labeled T cells in NOD.SCID mice.ResultsEstablished MR protocols were used for challenging T cell labeling to track inflammation in a model of diabetes after successful labeling of CD4+ and CD8+ T cells with PDP-PFCE NPs. However, T cells were difficult to be detected in vivo after their engraftment in animals.DiscussionWe showed successful in vitro labeling of T cells using novel fluorinated liposomal nanoparticles. However, insufficient and slow accumulation of labeled T cells and subsequent T cell proliferation in the pancreatic region remains as limitations of in vivo cell imaging by F-19 MRI.
- Keywords
- F-19 MRI, T cells, Inflammation, Nanoparticles, Type 1 diabetes, CELLS IN-VIVO, PANCREATIC-ISLETS, F-19 MRI, RESONANCE, INFLAMMATION, QUANTIFICATION, VISUALIZATION, MACROPHAGES, MIGRATION, CLONES
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8610983
- MLA
- Saini, Shweta, et al. “Challenges for Labeling and Longitudinal Tracking of Adoptively Transferred Autoreactive T Lymphocytes in an Experimental Type-1 Diabetes Model.” MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE, vol. 32, no. 3, 2019, pp. 295–305, doi:10.1007/s10334-018-0720-x.
- APA
- Saini, S., Korf, H., Liang, S., Verbeke, R., Manshian, B., Raemdonck, K., … Himmelreich, U. (2019). Challenges for labeling and longitudinal tracking of adoptively transferred autoreactive T lymphocytes in an experimental type-1 diabetes model. MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE, 32(3), 295–305. https://doi.org/10.1007/s10334-018-0720-x
- Chicago author-date
- Saini, Shweta, Hannelie Korf, Sayuan Liang, Rein Verbeke, Bella Manshian, Koen Raemdonck, Ine Lentacker, Conny Gysemans, Stefaan De Smedt, and Uwe Himmelreich. 2019. “Challenges for Labeling and Longitudinal Tracking of Adoptively Transferred Autoreactive T Lymphocytes in an Experimental Type-1 Diabetes Model.” MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE 32 (3): 295–305. https://doi.org/10.1007/s10334-018-0720-x.
- Chicago author-date (all authors)
- Saini, Shweta, Hannelie Korf, Sayuan Liang, Rein Verbeke, Bella Manshian, Koen Raemdonck, Ine Lentacker, Conny Gysemans, Stefaan De Smedt, and Uwe Himmelreich. 2019. “Challenges for Labeling and Longitudinal Tracking of Adoptively Transferred Autoreactive T Lymphocytes in an Experimental Type-1 Diabetes Model.” MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE 32 (3): 295–305. doi:10.1007/s10334-018-0720-x.
- Vancouver
- 1.Saini S, Korf H, Liang S, Verbeke R, Manshian B, Raemdonck K, et al. Challenges for labeling and longitudinal tracking of adoptively transferred autoreactive T lymphocytes in an experimental type-1 diabetes model. MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE. 2019;32(3):295–305.
- IEEE
- [1]S. Saini et al., “Challenges for labeling and longitudinal tracking of adoptively transferred autoreactive T lymphocytes in an experimental type-1 diabetes model,” MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE, vol. 32, no. 3, pp. 295–305, 2019.
@article{8610983,
abstract = {{Objective: Tracking the autoreactive T-cell migration in the pancreatic region after labeling with fluorinated nanoparticles (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[3-(2-pyridyldithio)propionate]-perfluoro-15-crown-5-ether nanoparticles, PDP-PFCE NPs) in a diabetic murine model using F-19 MRI.
Materials and methods: Synthesis of novel PDP-PFCE fluorine tracer was performed for in vitro labeling of T cells. Labeling conditions were optimized using different PDP-PFCE NPs concentrations. For in vivo F-19 MRI, mice were longitudinally followed after adoptive transfer of activated, autoreactive, labeled T cells in NOD.SCID mice.ResultsEstablished MR protocols were used for challenging T cell labeling to track inflammation in a model of diabetes after successful labeling of CD4+ and CD8+ T cells with PDP-PFCE NPs. However, T cells were difficult to be detected in vivo after their engraftment in animals.DiscussionWe showed successful in vitro labeling of T cells using novel fluorinated liposomal nanoparticles. However, insufficient and slow accumulation of labeled T cells and subsequent T cell proliferation in the pancreatic region remains as limitations of in vivo cell imaging by F-19 MRI.}},
author = {{Saini, Shweta and Korf, Hannelie and Liang, Sayuan and Verbeke, Rein and Manshian, Bella and Raemdonck, Koen and Lentacker, Ine and Gysemans, Conny and De Smedt, Stefaan and Himmelreich, Uwe}},
issn = {{0968-5243}},
journal = {{MAGNETIC RESONANCE MATERIALS IN PHYSICS BIOLOGY AND MEDICINE}},
keywords = {{F-19 MRI,T cells,Inflammation,Nanoparticles,Type 1 diabetes,CELLS IN-VIVO,PANCREATIC-ISLETS,F-19 MRI,RESONANCE,INFLAMMATION,QUANTIFICATION,VISUALIZATION,MACROPHAGES,MIGRATION,CLONES}},
language = {{eng}},
number = {{3}},
pages = {{295--305}},
title = {{Challenges for labeling and longitudinal tracking of adoptively transferred autoreactive T lymphocytes in an experimental type-1 diabetes model}},
url = {{http://doi.org/10.1007/s10334-018-0720-x}},
volume = {{32}},
year = {{2019}},
}
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